Cold Physical Plasma-Treated Buffered Saline Solution as Effective Agent Against Pancreatic Cancer Cells

Bekeschus, Sander; Käding, André; Schröder, Tim; Wende, Kristian; Hackbarth, Christine; Liedtke, Kim Rouven; Linde, Julia van der; Woedtke, Thomas von; Heidecke, Claus–Dieter; Partecke, Lars-Ivo

doi:10.2174/1871520618666180507130243

Cited by 27 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies by others and us have observed plasma-induced tumor cell death before [82][83][84][85][86][87][88][89][90][91][92][93][94][95][96][97][98]. Kumar and colleagues observed in several pancreatic cancer cell lines an increase in apoptosis concomitant with downregulation of MAPK7, BCL2, and CHK1 [99].…”

Section: Discussionmentioning

confidence: 94%

Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

Recent advances in melanoma therapy increased median survival in patients. However, death rates are still high, motivating the need of novel avenues in melanoma treatment. Cold physical plasma expels a cocktail of reactive species that have been suggested for cancer treatment. High species concentrations can be used to exploit apoptotic redox signaling pathways in tumor cells. Moreover, an immune-stimulatory role of plasma treatment, as well as plasma-killed tumor cells, was recently proposed, but studies using primary immune cells are scarce. To this end, we investigated the role of plasma-treated murine B16F10 melanoma cells in modulating murine immune cells’ activation and marker profile. Melanoma cells exposed to plasma showed reduced metabolic and migratory activity, and an increased release of danger signals (ATP, CXCL1). This led to an altered cytokine profile with interleukin-1β (IL-1β) and CCL4 being significantly increased in plasma-treated mono- and co-cultures with immune cells. In T cells, plasma-treated melanoma cells induced extracellular signal-regulated Kinase (ERK) phosphorylation and increased CD28 expression, suggesting their activation. In monocytes, CD115 expression was elevated as a marker for activation. In summary, here we provide proof of concept that plasma-killed tumor cells are recognized immunologically, and that plasma exerts stimulating effects on immune cells alone.

show abstract

Section: Discussionmentioning

confidence: 94%

Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…[ 106 ] Besides the ICD‐inducing nature of plasma‐mediated tumor cell death shown in our present work, gas plasma treatment but not a positive control failed to induce autoimmune events in a previous study in vivo [ 107 ] that would have occurred in case of an overshooting immune reaction after plasma exposure. In addition, we recently established tumor‐toxic gas plasma treatment [ 108 ] to be void of pro‐metastatic effects in four human pancreatic cancer cell lines. [ 109 ]…”

Section: Discussionmentioning

confidence: 99%

Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion

et al. 2020

Self Cite

View full text Add to dashboard Cite

Medical technologies from physics are imperative in the diagnosis and therapy of many types of diseases. In 2013, a novel cold physical plasma treatment concept was accredited for clinical therapy. This gas plasma jet technology generates large amounts of different reactive oxygen and nitrogen species (ROS). Using a melanoma model, gas plasma technology is tested as a novel anticancer agent. Plasma technology derived ROS diminish tumor growth in vitro and in vivo. Varying the feed gas mixture modifies the composition of ROS. Conditions rich in atomic oxygen correlate with killing activity and elevate intratumoral immune‐infiltrates of CD8+ cytotoxic T‐cells and dendritic cells. T‐cells from secondary lymphoid organs of these mice stimulated with B16 melanoma cells ex vivo show higher activation levels as well. This correlates with immunogenic cancer cell death and higher calreticulin and heat‐shock protein 90 expressions induced by gas plasma treatment in melanoma cells. To test the immunogenicity of gas plasma treated melanoma cells, 50% of mice vaccinated with these cells are protected from tumor growth compared to 1/6 and 5/6 mice negative control (mitomycin C) and positive control (mitoxantrone), respectively. Gas plasma jet technology is concluded to provide immunoprotection against malignant melanoma both in vitro and in vivo.

show abstract

“…A multitude of investigations on plasmaliquid interactions has demonstrated the occurrence of ROS and RNS in liquid phases following plasma treatment (36,37). Moreover, it has been shown several times that liquids, such as water, physiological saline, or cell culture media become biologically effective following plasma treatment (100)(101)(102)(103)(104)(105). This underlines a key role of liquid phase composition for biological plasma effects.…”

Section: Redox Biology As the Scientific Grounding Of Plasma Medicinementioning

confidence: 99%

Plasma Medicine: A Field of Applied Redox Biology

Schmidt

Bekeschus

et al. 2019

In Vivo

Self Cite

221

153

View full text Add to dashboard Cite

Cold Physical Plasma-Treated Buffered Saline Solution as Effective Agent Against Pancreatic Cancer Cells

Abstract: Plasma-treated PBS inhibited cell growth in 2D and 3D models of cancer. These results may help facilitate the development of new plasma-derived anticancer agent with clinical relevance in the future.

Cited by 27 publications

References 0 publications

Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells

Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells

Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion

Plasma Medicine: A Field of Applied Redox Biology

Contact Info

Product

Resources

About