Coinfection of Dermacentor silvarum Olenev (Acari: Ixodidae) by Coxiella-Like, Arsenophonus-Like, and Rickettsia-Like Symbionts

Liu, Limeng; Li, Lingxia; Liu, Jiannan; Hu, Yonghong; Liu, Zhao; Guo, Lida; Liu, Jingze

doi:10.1128/aem.03575-12

Cited by 29 publications

(21 citation statements)

References 44 publications

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“…The results are consistent with reports identifying Coxiella -like bacteria or endosymbionts in a number of Haemaphysalis , Dermacentor , and Amblyomma tick species (Lee et al 2004, Jasinskas et al 2007, Klyachko et al 2007, Liu et al 2013, Arthan et al 2015, Duron et al 2015). Several female ticks of Haemaphysalis and Amblyomma sampled here were fully engorged, hence it is possible that any Coxiella DNA observed may have originated from host blood.…”

Section: Discussionsupporting

confidence: 90%

Coxiella Detection in Ticks from Wildlife and Livestock in Malaysia

Khoo

Lim

Chen

et al. 2016

Vector-Borne and Zoonotic Diseases

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Recent studies have shown that ticks harbor Coxiella-like bacteria, which are potentially tick-specific endosymbionts. We recently described the detection of Coxiella-like bacteria and possibly Coxiella burnetii in ticks found from rural areas in Malaysia. In the present study, we collected ticks, including Haemaphysalis bispinosa, Haemaphysalis hystricis, Dermacentor compactus, Dermacentor steini, and Amblyomma sp. from wildlife and domesticated goats from four different locations in Malaysia. Coxiella 16s rRNA genomic sequences were detected by PCR in 89% of ticks tested. Similarity analysis and phylogenetic analyses of the 16s rRNA and rpoB partial sequences were performed for 10 representative samples selected based on the tick species, sex, and location. The findings here suggested the presence of C. burnetii in two samples, each from D. steini and H. hystricis. The sequences of both samples clustered with published C. burnetii sequences. The remaining eight tick samples were shown to harbor 16s rRNA sequences of Coxiella-like bacteria, which clustered phylogenetically according to the respective tick host species. The findings presented here added to the growing evidence of the association between Coxiella-like bacteria and ticks across species and geographical boundaries. The importance of C. burnetii found in ticks in Malaysia warrants further investigation.

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Section: Discussionsupporting

confidence: 90%

Coxiella Detection in Ticks from Wildlife and Livestock in Malaysia

Khoo

Lim

Chen

et al. 2016

Vector-Borne and Zoonotic Diseases

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“…These results have indicated that these bacteria are considered as predominant bacteria in ticks in China, consistent with previous studies (Gao et al 2010;Liu et al 2013;Sun et al 2015). The present and previous results (Moreno et al 2006;Tveten and Sjåstad, 2011;Xu et al 2015) have demonstrated that bacterial diversity differed significantly in different tick species.…”

Section: Discussionsupporting

confidence: 94%

PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks

Cheng

2017

Acta Parasitologica

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Ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. In the present study, the intestinal bacterial flora associated with Haemaphysalis flava ticks in different developmental stages were analyzed using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). Eleven distinct DGGE bands were found using PCR-DGGE method. Sequences analyses indicated that they belonged to Bacillus cereus, Candidatus rickettsia, Erwinia sp., Klebsiella pneumoniae, Pectobacterium carotovorum, Pseudomonas aeruginosa, Rickettsia peacockii, Rickettsia helvetica, Rickettsia slovaca, Staphylococcus simulans and Uncultured bacterium clone. Our find that the K. pneumoniae and P. aeruginosa isolates were presented in all H. flava ticks in different developmental stages. The present results indicated that zoonotic pathogens are present in H. flava ticks in Henan province, China. To our knowledge, this is the first report on intestinal bacterial flora associated with H. flava ticks in China.

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“…Rickettsia bacteria shared the highest sequence similarity (99.8%) with symbiotic Rickettsia of D. varibilis (GenBank: U55820) [54]. In males, the second abundant microbes (designated as ALS-Hl) belonged to Arsenophonus , sharing over 99.5% and 97.9% similarity to symbionts of D. silvarum (GenBank: JN866582) [55] and D. variabilis (GenBank: AY265342) [56], respectively . Following ALS-Hl, there were RLS-Hl and NCLS-Hl.…”

Section: Resultsmentioning

confidence: 99%

“…It is now widely accepted that the co-infection with multiple symbionts occurs in many arthropod hosts [60]. However, co-infection with symbionts has been demonstrated only in a few tick species, such as A. americanum [5], Ornithodoros moubata [61], D. silvarum [55] and Rh. sanguineus [62].…”

Section: Discussionmentioning

confidence: 99%

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Microbial communities and symbionts in the hard tick Haemaphysalis longicornis (Acari: Ixodidae) from north China

Liu

et al. 2013

Parasites Vectors

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BackgroundClose relationships between ticks and microbial communities are important for tick fitness and pathogen colonization and transmission. Haemaphysalis longicornis, distributed widely in China, can carry and transmit various pathogens and pose serious damages to public health and economics. However, little is known about the broader array of microbial communities and symbionts in H. longicornis under natural conditions. In the present study, we investigated the composition of bacterial communities associated with H. longicornis and evaluated the putative symbionts.MethodsThe eubacterial 16S rRNA gene clone libraries of H. longicornis were constructed and analyzed by restriction fragment length polymorphism (RFLP) and DNA sequencing. In addition, diagnostic PCR was performed to assess the prevalence, vertical transmission and infection sites of the symbionts in H. longicornis.ResultsVertically-transmitted symbionts, potential pathogens and allochthonous nonpathogenic bacteria were identified from the field-collected H. longicornis. Three types of symbionts (Coxiella-like, Arsenophonus-like and Rickettsia-like symbionts) were identified in a single host simultaneously. A series of analyses revealed the vertical transmission, prevalence, and infection sites of these symbionts. However, only Coxiella-like bacteria were transmitted stably in the laboratory-reared ticks. In addition, we identified a novel Coxiella-like agent with 95.31% sequence similarity to the taxon described previously.ConclusionsThe present study demonstrated that natural H. longicornis harboured a diverse array of microbial communities. Three types of symbionts were identified in a single host simultaneously. Moreover, high prevalence, vertical transmission and the infection sites supported an obligate symbiotic association between Coxiella symbiont and its host. The role of Coxiella symbiont in the host fitness and the interaction among microbial communities remained to be elucidated. Our investigation of microbial communities in the ticks revealed the complexity of ecological interactions between host and microbe and provided insight for the biological control of ticks.

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Coinfection of Dermacentor silvarum Olenev (Acari: Ixodidae) by Coxiella-Like, Arsenophonus-Like, and Rickettsia-Like Symbionts

Cited by 29 publications

References 44 publications

Coxiella Detection in Ticks from Wildlife and Livestock in Malaysia

Coxiella Detection in Ticks from Wildlife and Livestock in Malaysia

PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks

Microbial communities and symbionts in the hard tick Haemaphysalis longicornis (Acari: Ixodidae) from north China

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