Cohnella
               
            
            formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L

Hameed, Asif; Hung, Mei-Hua; Lin, Shih-Yao; Hsu, Yi-Han; Liu, You‐Cheng; Shahina, Mariyam; Lai, Wei-An; Huang, Hsin‐Chieh; Young, Li-Sen; Young, Chiu-Chung

doi:10.1099/ijs.0.045831-0

Cited by 23 publications

(10 citation statements)

References 33 publications

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“…The prevalence of this genus may be a sign of their numerical abundance; nevertheless, bias due to the procedures and culture media used must be considered. Also belonging to Firmicutes , members of Paenibacillaceae family have been often described as strong cellulolytic and hemicellulolytic bacteria, reports which are in agreement to our observations . However, to our knowledge there are no previous reports of a zymographic profile revealed with CMC as substrate for Cohnella species.…”

Section: Discussionsupporting

confidence: 92%

Cellulose degrading bacteria isolated from industrial samples and the gut of native insects from Northwest of Argentina

Manfredi

Perotti

Martinez

2015

J. Basic Microbiol.

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The raw materials used to produce bioethanol mostly are food crops, which has led to conflicts on food security. It is, therefore, recommended the gradual replacement for second generation substrates such as lignocellulosic materials. Herein, cellulolytic bacteria were isolated from the gut content of native larvae from Lepidoptera, Coleoptera, and adults of Isoptera. Few environmental samples from the pulp and paper feedstock were also assessed. A total of 233 isolates were obtained using enrichment cultures and classic criteria. Interestingly, several halo-forming colonies were found to be bacterial consortia that presented difficulties to take apart the microbial members. Those pure isolates which hydrolyzed cellulose in larger extend (45 strains) were selected and identified by means of 16S rRNA sequence analysis. Firmicutes was the prevalent phylum (62.2%) being Bacillus spp. the most frequent genus, while Paenibacillus, Brevibacillus, Cohnella, and Staphylococcus species were less frequent. The phylum Actinobacteria (6.7%) was represented by isolates related to Agromyces spp. and Microbacterium spp. Regarding Gram-negative bacteria (31.1%), the more depicted genus was Pseudomonas spp., and members of Achromobacter spp., Enterobacter spp., and Bacteroidetes phylum were also selected. These native bacterial strains are expected to enlarge the cellulolytic toolbox for efficient biomass deconstruction.

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Section: Discussionsupporting

confidence: 92%

Cellulose degrading bacteria isolated from industrial samples and the gut of native insects from Northwest of Argentina

Manfredi

Perotti

Martinez

2015

J. Basic Microbiol.

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“…Major fatty acids are iso-C 16 : 0 , anteiso-C 15 : 0 and C 16 : 0 and the predominant polar lipids are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine (Kämpfer et al, 2006). At present, the genus contains 21 species with validly published names, Cohnella hongkongensis and C. thermotolerans (Kämpfer et al, 2006), C. laeviribosi (Cho et al, 2007), C. phaseoli (García-Fraile et al, 2008), C. damuensis (Luo et al, 2010), C. fontinalis (Shiratori et al, 2010), C. ginsengisoli and C. yongneupensis (Kim et al, 2010), C. luojiensis (Cai et al, 2010), C. thailandensis (Khianngam et al, 2010a), C. terrae and C. xylanilytica (Khianngam et al, 2010b), C. panacarvi (Yoon et al, 2007), C. cellulosilytica (Khianngam et al, 2012), C. soli and C. suwonensis (Kim et al, 2012), C. boryungensis (Yoon & Jung, 2012), C. arctica (Jiang et al, 2012), C. formosensis (Hameed et al, 2013), C. lupini (Flores-Félix et al, 2014) and C. ferri (Mayilraj et al, 2011), as well as 'Cohnella plantaginis' , the name of which has not yet been validly published. Most of the type strains were isolated from different soils (Yoon et al, 2007;Cai et al, 2010;Kim et al, 2010;Kim et al, 2012;Jiang et al, 2012;Yoon & Jung 2012;Khianngam et al, 2010a, b); some were isolated from the rhizosphere (Hameed et al, 2013) or endophytic compartments, root nodules, of plants (FloresFélix et al, 2014;García-Fraile et al, 2008).…”

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confidence: 99%

Cohnella rhizosphaerae sp. nov., isolated from the rhizosphere environment of Zea mays

Kämpfer

Glaeser

McInroy

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-staining-positive, aerobic, non-endospore forming organism, isolated as a seed endophyte (colonizing the internal healthy tissue of plant seed) of sweet corn (Zea mays), strain CSE-5610 T , was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence comparisons, strain CSE-5610 T was grouped into the genus Cohnella, most closely related toCohnella ginsengisoli GR21-5 T (98.1 %) and 'Cohnella plantaginis' YN-83 (97.5 %). The 16SrRNA gene sequence similarity to other members of the genus Cohnella was ,96.6 %. DNA-DNA hybridization of strain CSE-5610 T with C. ginsengisoli DSM 18997 T and 'C. plantaginis' DSM 25424 was 58 % (reciprocal 24 %) and 30 % (reciprocal 27 %), respectively. The fatty acid profile from whole cell hydrolysates supported the allocation of the strain to the genus Cohnella; iso-and anteiso-branched fatty acids were found as major compounds. meso-Diaminopimelic acid was identified as the cell-wall diamino acid. The quinone system consisted predominantly of menaquinone MK-7. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, a phospholipid and minor amounts of two polar lipids. In the polyamine pattern, spermidine was the major polyamine. The G+C content of the genomic DNA was 60 mol%. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain CSE-5610 T from the two closely related strains. Hence, CSE-5610 T represents a novel species of the genus Cohnella, for which we propose the name Cohnella rhizosphaerae sp. nov., with CSE-5610 T (5LMG 28080 T 5CIP 110695 T ) as the type strain.

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“…Strain FAB2 T contained meso -diaminopimelic acid. This diamino acid has been commonly detected in some closely related species of the genera Cohnella (Khianngam et al , 2010a, 2010b, 2012; Hameed et al , 2013; Flores-Félix et al , 2014; Kämpfer et al , 2014; Huang et al , 2014) and Saccharibacillus (Yang et al , 2009). Genomic DNA was prepared using the same method used for PCR amplification of the 16S rRNA gene.…”

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confidence: 95%

Ammoniibacillus agariperforans gen. nov., sp. nov., a thermophilic, agar-degrading bacterium isolated from compost

Sakai

Deguchi

Hosoda

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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Ammoniibacillus agariperforans gen. nov., sp. nov., a thermophilic, agar-degrading bacterium isolated from compost T was different enough at the genus level from closely related species. The percentages of 16S rRNA gene sequence similarity with related organisms were 90.4 % for Thermobacillus xylanilyticus, 91.8 % for Paenibacillus barengoltzii, 89.4 % for Cohnella lupini, 90.1 % for Fontibacillus aquaticus, and 89.0 % for Saccharibacillus sacchari. Morphological and physiological analyses revealed that the strain was motile, rod-shaped, Gram-stain-positive, aerobic and able to form oval endospores in swollen sporangia. Ammonium was required as a nitrogen source while nitrate, nitrite, urea and glutamate were not utilized. Catalase and oxidase activities were weakly positive and positive, respectively. The bacterium grew in the temperature range of 50-65 6C and in media with pH 7.5 to 9.0. Optimal growth occurred at 60 6C and pH 8.0-8.6. Growth was inhibited at pH¡7.0 and NaCl concentrations ¢2.5 % (w/v). In chemotaxonomic characterization, MK-7 was identified as the dominant menaquinone. Major fatty acids were iso-C 16 : 0 and C 16 : 0 . Dominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phosphatidylcholine was present in a moderate amount. The diamino acid in the cell wall was meso-diaminopimelic acid. The G+C content of the genomic DNA was 49.5 mol% in a nucleic acid study. On the basis of genetic and phenotypic characteristics, strain FAB2showed characteristics suitable for classification as the type strain of a novel species of a new genus in the family Paenibacillaceae, for which the name Ammoniibacillus agariperforans gen. nov., sp. nov. is proposed.

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Cohnella formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L

Cited by 23 publications

References 33 publications

Cellulose degrading bacteria isolated from industrial samples and the gut of native insects from Northwest of Argentina

Cellulose degrading bacteria isolated from industrial samples and the gut of native insects from Northwest of Argentina

Cohnella rhizosphaerae sp. nov., isolated from the rhizosphere environment of Zea mays

Ammoniibacillus agariperforans gen. nov., sp. nov., a thermophilic, agar-degrading bacterium isolated from compost

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