Coherent control of fluorescent proteins with a compact high-resolution spectral phase shaper

Postma, S.; Subramaniam, Vinod; Hulst, N.F. van; Offerhaus, Herman L.

doi:10.1364/up.2006.tug13

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…The gene cloned fluorescent proteins used as tracers to observe many biomedical phenomena have been widely applied in the biological and clinical observations [1][2][3][4][5][6][7] . The advantages include the high S/N ratio and indirect illumination which is significant in organism observation comparing to other techniques.…”

Section: Introductionmentioning

confidence: 99%

Dynamically monitoring the gene expression of dual fluorophore in the cell cycle with quantitative spectrum analysis

Lee

Hsu

2008

SPIE Proceedings

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The cloning and transcription techniques on gene cloned fluorescent proteins have been widely used in many applications. They have been used as reporters of some conditions in a series of reactions. However, it is usually difficult to monitor the specific target with the exactly number of proteins during the process in turbid media, especially at micrometer scales. We successfully revealed an alternative way to monitor the cell cycle behavior and quantitatively analyzed the target cells with green and red fluorescent proteins (GFP and RFP) during different phases of the cell cycle by quantitatively analyzing its behavior and also monitoring its spatial distribution.

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Section: Introductionmentioning

confidence: 99%

Dynamically monitoring the gene expression of dual fluorophore in the cell cycle with quantitative spectrum analysis

Lee

Hsu

2008

SPIE Proceedings

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“…Among the variety of exogenous fluorescent agents, green fluorescent protein (GFP) possesses the advantages of robustness and high quantum efficiency [5][6][7] . Therefore, GFP has become indispensable in molecular and cell biology as noninvasive luminescent labels for monitoring gene expression, protein localization and protein interactions [8][9][10][11][12][13] . Although GFP and its mutants have been used in many applications, their optical properties and reactions have not been completely investigated, especially when they are under various environmental conditions and different cloning representation genes by virus infection.…”

Section: Introductionmentioning

confidence: 99%

Optical properties of green fluorescent proteins and their applications on virus infection

et al. 2007

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Exogenous fluorescent agents such as green fluorescent protein (GFP) have been widely used as biological indicators in bioimaging techniques. Although GFP and its mutants have been used in many applications, their optical properties have not been completely investigated, especially when they are under various environmental conditions. In this research, we developed a spectrum-analyzing system to investigate the fluorescent properties of GFP in the environments of different temperatures. We found that the fluorescent spectrum of GFP consisted of two components that might come from the transitions between different electronic energy states where the quantum efficiencies of the two components varied with different temperature. This effect was expected to come from the thermal effect on the electron populations in the molecular energy states of GFP. Furthermore, GFP was used as fluorescent marker to monitor the infection process of cells by viruses with a dynamic spectral imaging system. The recombinant baculoviruses containing the red and green fluorescent protein gene that can simultaneously produce dual fluorescence were used as vectors in insect cells. The system was used to monitor the spatial distribution of fluorescent spectra of cells infected by virus during the process of infection. Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/25/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx SPIE-OSA/ Vol. 6626 662606-2 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/25/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx SPIE-OSA/ Vol. 6626 662606-9 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 06/25/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx

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Monitoring and quantification of the protein partition during cytokinesis with fluorescent spectral imaging

Lee

Lin

et al. 2009

SPIE Proceedings

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Coherent control of fluorescent proteins with a compact high-resolution spectral phase shaper

Cited by 3 publications

References 4 publications

Dynamically monitoring the gene expression of dual fluorophore in the cell cycle with quantitative spectrum analysis

Dynamically monitoring the gene expression of dual fluorophore in the cell cycle with quantitative spectrum analysis

Optical properties of green fluorescent proteins and their applications on virus infection

Monitoring and quantification of the protein partition during cytokinesis with fluorescent spectral imaging

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