Coffee intake mitigated inflammation and obesity-induced insulin resistance in skeletal muscle of high-fat diet-induced obese mice

Abstract

Epidemiologic findings offer the promise that coffee or its many constituents may be useful as a dietary intervention in type 2 diabetes (T2D) prevention. We aimed to elucidate the molecular mechanisms involved in the ameliorative effects of caffeinated coffee (CC), decaffeinated coffee (DC) and unroasted caffeinated green coffee (GC) on skeletal muscle gene expression profiles and their relationships in an obesity animal model. Eight-week-old male C57BL6 mice were raised for 9 weeks ad libitum on a normal diet, a high-fat diet, or high-fat diet containing 2 % freeze-dried CC, or DC, or GC. Total RNA and protein were extracted from skeletal muscle and subjected to microarray (Mouse Genome 430 2.0, Affymetrix) and western blotting analyses, respectively. Coffee intake mitigated the insulin resistance by decreasing plasma glucose levels during an insulin tolerance test and by increasing tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1), p85/IRS-1 complex and pAkt/PKB (protein kinase B). In addition, coffee intake down-regulated the anti-inflammatory genes activating transcription factor 3, FBJ osteosarcoma oncogene, heat shock protein 1A, heat shock protein 1B and synuclein, gamma and the inflammation-associated insulin signaling genes stearoyl-coenzyme A desaturase 1 and secreted phosphoprotein 1. These results provide scientific insight on the probable positive effects of coffee intake on impaired insulin signaling, inflammation and obesity, thereby providing a new perspective on the prevention of obesity and T2D.

Genetic variation of habitual coffee consumption and glycemic changes in response to weight-loss diet intervention: the Preventing Overweight Using Novel Dietary Strategies (POUNDS LOST) trial

“…Several genetic variants have been identified as being related to habitual coffee consumption (4)(5)(6). Furthermore, data from experimental studies suggest that dietary macronutrients, such as fat intake, may modulate the effects of coffee consumption on insulin resistance and glucose metabolism (7,8). Whether macronutrient intakes modify the effects of the coffee consumption-associated genetic variants remains unclear.…”

Effects of Unfiltered Coffee and Bioactive Coffee Compounds on the Development of Metabolic Syndrome Components in a High‐Fat/High‐Fructose-Fed Rat Model

“…Cowan et al mirrored these results with a higher dosage of instant coffee using a similar animal model [23]. Modest weight modifying Peer-reviewed version available at Nutrients 2018, 10, 1547; doi:10.3390/nu10101547 of effects of coffee have also been observed in some [24,25] but not all [26,27] of the previous comparable studies. Caffeine is known to induce weight loss in rodents [28,29] and help to maintain weight loss in humans [30], but it may not be the only player.…”

“…Acute caffeine administration decreases insulin sensitivity [41]; however, in a time span of several weeks, this methylxanthine exhibits enhancing effects on glucose disposal rate [26,29]. Comparable FPG levels in all groups were not surprising based on the knowledge that this index of liver glucose production was either unaltered [24,25] or increased [23] in the former studies. Nevertheless, lower HbA1C in the coffee group signifies a better long-term glycemic control which is a better predictor of cardiovascular disease and all-cause mortality than the FPG [42].…”

Association between Type-2 diabetes and Parkinson’s disease: a cross-talk between amylin and α-synuclein

“…To the contrary, in skeletal muscle of high-fat diet-induced obese mice, -synuclein expression is significantly elevated [27] . Mechanism of involvement of -synuclein in diabetes is still unclear, but may be associated with its role in the regulation of metabolic processes in adipocytes and the maintenance of adipocyte lipid droplets and aberrant lipid metabolism [28] .…”