Coexistence of <i>Candida albicans</i> and <i>Enterococcus faecalis</i> increases biofilm virulence and periapical lesions in rats

Du, Qian; Yuan, Shasha; Zhao, Shuangyuan; Fu, Di; Chen, Yifei; Zhou, Yuan; Cao, Yangpei; Gao, Yuan; Xu, Xin; Zhou, Xuedong; He, Jinzhi

doi:10.1080/08927014.2021.1993836

Cited by 15 publications

(12 citation statements)

References 53 publications

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“…The same results are also seen for antimicrobial photodynamic therapy ( Diogo et al, 2017 ) and some new root canal medicaments (chitosan, silver nanoparticles, and ozonated olive oil) ( Elshinawy et al, 2018 ). Consistently, significantly increased extent of periapical alveolar bone damage area is observed in an in vivo E. faecalis and C. albicans co-infection rat model, compared with mono-species infection, in conjunction with the increase in proportion of osteoclasts and decrease in osteoblasts ( Du et al, 2021b ). Moreover, inflammatory cytokines (TNF-a and IL-6) in periapical lesions are also upregulated by E. faecalis / C. albicans co-infection ( Du et al, 2021b ).…”

Section: Interactions Between C Albicans and Oral ...mentioning

confidence: 62%

“…By scanning electron microscopy, it has been demonstrated that E. faecalis adheres to yeast and hyphal cells of C. albicans in infected tooth root canals as well as in dentinal tubules ( Siqueira and Rocas, 2009 ). In an in vitro study, co-culturing C. albicans and E. faecalis yields a thicker and denser biofilm compared with mono-species biofilm, which exhibits higher tolerance to detrimental stresses, including alkalinity starvation, mechanical shear force, and fungicide/bactericide ( Gao et al, 2016 ; Diogo et al, 2017 ; Du et al, 2021b ), such as sodium hypochlorite (NaClO), ethylenediamine tetraacetic acid (EDTA), and chlorhexidine gluconate (CHX) ( Diogo et al, 2017 ; Du et al, 2021b ). The same results are also seen for antimicrobial photodynamic therapy ( Diogo et al, 2017 ) and some new root canal medicaments (chitosan, silver nanoparticles, and ozonated olive oil) ( Elshinawy et al, 2018 ).…”

Section: Interactions Between C Albicans and Oral ...mentioning

confidence: 99%

“…Recent drug susceptibility studies reveal that the co-presence of C. albicans and oral bacteria in biofilms influences the susceptibility of either to antimicrobial agents. Eradicating Candida –bacterial polymicrobial biofilm-induced diseases is challenging ( Montelongo-Jauregui et al, 2016 ; Diogo et al, 2017 ; Elshinawy et al, 2018 ; Kim et al, 2018 ; Chinnici et al, 2019 ; Ikono et al, 2019 ; Du et al, 2021b ; Gong et al, 2021 ; Kulshrestha and Gupta, 2022 ). Montelongo-Jauregui et al (2016) reports that in both monotherapy and combination therapy with commonly used antifungals and antibacterial antibiotics, C. albicans / S. gordonii mixed-species biofilm becomes more resistant to antimicrobial treatments at all doses regardless of whether they are cultured on conventional media or synthetic saliva.…”

Section: Antimicrobial Resistancementioning

confidence: 99%

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Cross-kingdom interaction between Candida albicans and oral bacteria

Ren

Zhou

et al. 2022

Front. Microbiol.

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Candida albicans is a symbiotic fungus that commonly colonizes on oral mucosal surfaces and mainly affects immuno-compromised individuals. Polymicrobial interactions between C. albicans and oral microbes influence the cellular and biochemical composition of the biofilm, contributing to change clinically relevant outcomes of biofilm-related oral diseases, such as pathogenesis, virulence, and drug-resistance. Notably, the symbiotic relationships between C. albicans and oral bacteria have been well-documented in dental caries, oral mucositis, endodontic and periodontal diseases, implant-related infections, and oral cancer. C. albicans interacts with co-existing oral bacteria through physical attachment, extracellular signals, and metabolic cross-feeding. This review discusses the bacterial–fungal interactions between C. albicans and different oral bacteria, with a particular focus on the underlying mechanism and its relevance to the development and clinical management of oral diseases.

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Section: Interactions Between C Albicans and Oral ...mentioning

confidence: 62%

Section: Interactions Between C Albicans and Oral ...mentioning

confidence: 99%

Section: Antimicrobial Resistancementioning

confidence: 99%

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Cross-kingdom interaction between Candida albicans and oral bacteria

Ren

Zhou

et al. 2022

Front. Microbiol.

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“…The possibility of spreading the applied approach, namely, combining QQE with antibiotics, to the development of highly effective antimicrobial drugs seems simple and attractive not only for use against bacteria, but also to cells of other microorganisms, including those involved in the formation of various heterogeneous consortia in the form of biofilms based on pro- and eukaryotes, in particular, bacteria and yeast of the genus Candida [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“Universal” Antimicrobial Combination of Bacitracin and His6-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells

Aslanli

Domnin

Stepanov

et al. 2022

IJMS

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The effect of Bacitracin as an antibiotic acting against Gram-positive bacterial cells was evaluated in combination with hexahistidine-containing organophosphate hydrolase (His6-OPH), possessing lactonase activity against various N-acylhomoserine lactones produced by most Gram-negative bacteria as quorum-sensing molecules. The molecular docking technique was used to obtain in silico confirmation of possible interactions between molecules of His6-OPH and Bacitracin as well as the absence of a significant influence of such interactions on the enzymatic catalysis. The in vitro experiments showed a sufficient catalytic efficiency of action of the His6-OPH/Bacitracin combination as compared to the native enzyme. The notable improvement (up to 3.3 times) of antibacterial efficiency of Bacitracin was revealed in relation to Gram-negative bacteria when it was used in combination with His6-OPH. For the first time, the action of the Bacitracin with and without His6-OPH was shown to be effective against various yeast strains, and the presence of the enzyme increased the antibiotic effect up to 8.5 times. To estimate the role of the enzyme in the success of His6-OPH/Bacitracin with yeast, in silico experiments (molecular docking) with various fungous lactone-containing molecules were undertaken, and the opportunity of their enzymatic hydrolysis by His6-OPH was revealed in the presence and absence of Bacitracin.

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“…Mandibles were dissected under a microscope and then fixed in 4% paraformaldehyde overnight at room temperature. Scanning was done with a micro-CT (μCT50; SCANCO) to analyze periapical bone resorption as we did previously [ 22 ]. Scanning was performed at 70 kV and 200 mA with 300 ms integration time.…”

Section: Methodsmentioning

confidence: 99%

Human periodontitis-associated salivary microbiome affects the immune response of diabetic mice

Shen²,

Fu³

et al. 2022

Journal of Oral Microbiology

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Background The bidirectional association between periodontitis and diabetes mellitus has been well accepted; however, pathways connecting them remain unclear. Some oral bacteria are able to induce immunologic changes favoring insulin resistance individually. However, it is unclear if and how the systemic immune system responds to a disturbed oral microbial community in diabetic sufferers. Aim This study aimed to investigate the impact of the human periodontitis-associated salivary microbiome on the splenic immune responses of diabetic mice. Methods An in vivo diabetic animal model was established by feeding high fat food. After microbial depletion with quadruple antibiotic treatment, human saliva from healthy and periodontitis volunteers was transplanted into the mouth of these diabetic mice (N = 3), respectively. Results Osteoclasts and expression levels of TNF-α and IL-1β were significantly increased in periodontal tissues of mice receiving periodontitis patients donated microbiome compared to these transplanted with healthy subjects donated microbiome. The proportion of monocyte (an innate immunocyte) decreased in mice receiving periodontitis patients donated microbiome. However, the abundance of an adaptive immunocyte Th17 was up-regulated. The IL17 production of ILC3 cells in human periodontitis-associated salivary microbiome recipient mice was significantly impaired. Conclusions A disturbed oral microbiome imposes a stress on the splenic immune responses of diabetic mice.

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Coexistence of Candida albicans and Enterococcus faecalis increases biofilm virulence and periapical lesions in rats

Cited by 15 publications

References 53 publications

Cross-kingdom interaction between Candida albicans and oral bacteria

Cross-kingdom interaction between Candida albicans and oral bacteria

“Universal” Antimicrobial Combination of Bacitracin and His6-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells

Human periodontitis-associated salivary microbiome affects the immune response of diabetic mice

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