Coexistence of cholecystokinin- and substance P-like peptides in neurons of the dorsal root ganglia of the rat

Dalsgaard, C.‐J.; Vincent, S.R.; Hökfelt, Tomas; Lundberg, Jan M.; Dahlström, Annica; Schultzberg, Marianne; Dockray, Graham J.; Cuello, A. Claudio

doi:10.1016/0304-3940(82)90244-0

Cited by 138 publications

(22 citation statements)

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“…Since our results indicate the presence of CCK-IR fibers in the spinal cord, it is reasonable to suppose that the small number of neurons with CCK in the cytoplasm of the somata would increase if treatment with colchicine were applied. Our data agree with what has been reported in mouse (Sommer et al, 1985), rat (Dalsgaard et al, 1982;Ju et al, 1987), guinea-pig (Gibbins et al, 1987), and cat (Hökfelt et al, 1975) in which SP-IR neurons in the DRGs belonged to small and medium sized classes and were more numerous than CCK-IR cells. Our results suggest also that the distribution of SP-IR and CCK-IR fibers in the spinal cord of Tursiops truncatus is similar to that reported for other species, including the rat and monkey (Ljungdahl et al, 1978;Battaglia and Rustioni, 1988).…”

Section: Discussionsupporting

confidence: 93%

Immunohistochemical Localization of Substance P And Cholecystokinin in the Dorsal Root Ganglia and Spinal Cord of the Bottlenose Dolphin (Tursiops truncatus)

Bombardi

Grandis

Nenzi

et al. 2010

The Anatomical Record

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The presence of substance P (SP) and cholecystokinin (CCK) immunoreactive neurons was examined in the bottlenose dolphin dorsal root ganglia (DRGs) and spinal cord by immunohistochemical techniques. SPpositive and CCK-immunoreactive neurons were respectively $50% and 1% of the total number of ganglion cells examined and especially belonged to small and medium-sized cell populations. Using double labeling techniques we observed that SP-and CCK-immunoreactivity coexisted in a very low number of primary afferent neurons (2.7%). Few SPimmunoreactive (IR) neurons (2.7%) were also CCK-positive. On the contrary, 65% of CCK-immunoreactive neurons contained SP. Interestingly, we observed CCK-immunoreactive satellite glial cells located around large cell class somata. Virtually no SP-IR and CCK-positive neurons were surrounded by peripheral CCK-immunoreactive satellite glial cells. The SP-IR and CCK-positive nerve fibers were particularly conspicuous in the superficial layers of the spinal cord. The present study indicates that SP and CCK only partially overlap in the thoracic, lumbar, and caudal DRGs of the bottlenose dolphin, suggesting that the majority of SP-IR ganglion neurons are lacking in CCK-immunoreactivity. The role of SPcontaining DRG neurons is discussed also in relation to the huge vascular spinal retia mirabilia typical of cetaceans. Anat Rec, 293:477-484, 2010. V V C 2010 Wiley-Liss, Inc.

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Section: Discussionsupporting

confidence: 93%

Immunohistochemical Localization of Substance P And Cholecystokinin in the Dorsal Root Ganglia and Spinal Cord of the Bottlenose Dolphin (Tursiops truncatus)

Bombardi

Grandis

Nenzi

et al. 2010

The Anatomical Record

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“…show preference for the NK,, NK2 and NK3 tachykinin receptor subtypes, respectively (reviewed by Otsuka & Yoshioka, 1993 (Webber, 1989) and 0 34 nm for airway macromolecular secretion (Gentry, 1991 (Dalsgaard, et al 1982;Lindh, Dalsgaard, Elfvin, Hokfelt & Cuello, 1983), trigeminal root (McCarthy & Lawson, 1989), jugular (Katz & Karten, 1980) and nodose ganglia (Katz & Karten, 1980;Helke & Hill, 1988;Kummer et al 1992). Activation of these neurones elicits release of mediators, including tachykinins such as SP, from the sensory nerve terminals in the CNS and in the periphery (Saria et al 1988).…”

Section: A Controlmentioning

confidence: 99%

Substance P hyperpolarizes vagal sensory neurones of the ferret.

Jafri

Weinreich

1996

The Journal of Physiology

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1. Intracellular recordings were made in intact and in acutely dissociated vagal afferent neurones (nodose ganglion cells) of the ferret to investigate the effects of substance P (SP). 2. In current-clamp recordings, SP (100 nM) applied by superfusion hyperpolarized the membrane potential (7 + 0 7 mV; mean + S.E.M.; n = 105) and decreased the input resistance in 80 % of the neurones. With voltage-clamp recording, SP produced an outward current of 3 + 0-2 nA (n = 10). 3. The SP current was concentration dependent with an estimated EC50 of 68 nm. The SP-induced hyperpolarization or current was mimicked by the tachykinin receptor NK1agonist Ac-[Arg6, Sar9, Met(02)'1]SP(6-1 1) (ASM-SP; 100 nM; n = 10) and blocked by the NK1 antagonist CP-96,345 (10 nM; n = 6), but not by the NK2 antagonist SR48968(100 nM; n = 4). No measurable change in membrane potential or input resistance was observed with application of either [/J-Ala8]neurokinin A or senktide, selective NK2 and NK3 receptor agonists, respectively (100 nM; n = 3 for each agonist).4. The reversal potential (Erev) for the SP outward current was -85 + 2-5 mV (n = 4). The Erev for the SP response shifted in a Nernstian manner with changes in extracellular potassium concentration. Alterations in extracellular sodium or chloride concentrations had no significant effect on the E for the SP response (n = 3 for each ion).5. Nominally Ca2+-free external solution abolished the SP response. Removal of magnesium from the extracellular solution had no effect on the response.6. Caesium (100 /,M), barium (1 mM), tetraethylammonium (TEA; 5 mm), apamin (10 nM) and 4-aminopyridine (4-AP; 4 mm) each completely prevented the SP response (n > 3 for each).7. These results indicate that SP, via an NK1 receptor, can induce a Ca2+-dependent outward potassium current which hyperpolarizes the resting membrane potential of vagal afferent somata.

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“…On the other hand, comprehensive retrograde tracing investigations were performed in other female genital organs including guinea pig [Alm and Lundberg, 1988], pig [Wąsowicz et al, 1998], rat [Inyama et al, 1986;Papka, 1990] and cat uterus [Kawatani and de Groat, 1991] was well as on the rat ovary [Inyama et al, 1986;McNeill and Burden, 1986] and pig ovary [Majewski and Heym, 1991;Majewski et al, 1991]. It has been found that thoracolumbar DRG neurons contain different combinations of biologically active substances including substance P (SP) and calcitonin gene-related peptide (CGRP) [Gibbins et al, 1985[Gibbins et al, , 1987Ju et al, 1987;Alm and Lundberg, 1988;Merighi et al, 1988Merighi et al, , 1990Lindh et al, 1989;Majewski et al, 1996], SP and galanin [Papka and Traurig, 1989;Majewski et al, 1996], SP and cholecystokinin [Dalsgaard et al, 1982;Gibbins et al, 1987], SP and nitric oxide synthase (NOS) [Aimi et al, 1991;Zhang et al, 1993;Heym et al, 1995;Majewski et al, 1995], SP and somatostatin [Leah et al, 1985;Merighi et al, 1988;Garry et al, 1989;Hanesch et al, 1995;Kashiba et al, 1996] or CGRP and vasoactive intestinal peptide (VIP) [Villar et al, 1989;Noguchi et al, 1993;Isaacs et al, 1995]. However, there is a paucity of data dealing with the origin [Wessendorf and Elde, 1985], the present study was aimed at disclosing the distribution of 'oviductal' primar...…”

Section: Introductionmentioning

confidence: 99%

Distribution of Primary Afferent Neurons Innervating the Porcine Oviduct and Their Immunohistochemical Characterization

Czaja¹

2000

Cells Tissues Organs

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Previous studies have revealed that some nerve fibres supplying the porcine oviduct may be of sensory origin. Therefore, the present study was aimed at disclosing the distribution of porcine ‘oviductal’ primary afferent neurons and the pattern(s) of putative coincidence of substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) within these nerve cell bodies using combined retrograde tracing and double-labelling immunohistochemistry. We also investigated the existence and coexistence of immunoreactivities to tyrosine hydroxylase (TH) and dopamine β-hydroxylase within the neurons because in some mammals, dorsal root ganglia (DRG) were previously found to contain perikarya immunoreactive (IR) to TH. Retrograde labelling revealed a population of large sensory neurons located in the Th₁₀–L₃ DRG. There were no significant differences in the number or distribution between the ampulla- and isthmus-projecting neurons. Double-labelling immunoflourescence allowed several subpopulations of the studied perikarya to be distinguished. The largest one consisted of SP/CGRP-IR nerve cells, while the smallest subpopulation comprised NOS/VIP-IR neurons. Either SP/NOS, solely SP- or solely NOS-IR neurons were also found. Because identically coded nerve fibres have been observed within the wall of the porcine oviduct, based on their association with particular organ structures, it can be assumed that SP/CGRP-, SP/NOS- or solely NOS-IR neurons are involved in the antidromic relaxation of the oviductal vessels, SP-, NOS- or SP/CGRP-IR nerve cells control the oviductal tonus and that some neurons project beneath the epithelium and are involved in the transmission of sensory modalities from the oviduct to the spinal cord.

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Coexistence of cholecystokinin- and substance P-like peptides in neurons of the dorsal root ganglia of the rat

Cited by 138 publications

References 0 publications

Immunohistochemical Localization of Substance P And Cholecystokinin in the Dorsal Root Ganglia and Spinal Cord of the Bottlenose Dolphin (Tursiops truncatus)

Immunohistochemical Localization of Substance P And Cholecystokinin in the Dorsal Root Ganglia and Spinal Cord of the Bottlenose Dolphin (Tursiops truncatus)

Substance P hyperpolarizes vagal sensory neurones of the ferret.

Distribution of Primary Afferent Neurons Innervating the Porcine Oviduct and Their Immunohistochemical Characterization

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