Codonopis�bulleynana Forest ex Diels inhibits autophagy and induces apoptosis of colon cancer cells by activating the NF-κB signaling pathway

Abstract

Despite its favorable clinical efficacy, oxaliplatin-based chemotherapy frequently results in treatment withdrawal and induces liver damage in colon cancer. Therefore, it is important to develop novel drugs, which can safely and effectively complement or replace the therapeutic effects of oxaliplatin. Codonopis bulleynana Forest ex Diels (cbFeD) has wide range of pharmacological effects, including anticancer effects. In the present study, the anticancer activity of cbFeD and its potential molecular mechanisms were investigated. In vitro, cell counting kit-8 assays and flow cytometry were used to assess the anti-proliferation and apoptosis-promoting activities of cbFeD. Transmission electron microscopy was used to monitor the autophagic vesicles. Immunofluorescence staining was performed to observe the nuclear translocation of p65 and the fluorescence of microtubule-associated protein 1 light chain 3 (LC3) B-II. The protein expression levels of p65, inhibitor of nuclear factor (NF)-κB (IκB) a, LC3B-I, LC3B-II and Beclin-1 were detected using western blot analysis. In vivo, the antitumor effect of cbFeD was assessed in colon cancer-bearing nude mice as a model. H&E staining and immunohistochemistry (IHC) were performed, with oxaliplatin set as a positive control. The results showed that cbFeD inhibited cell proliferation and promoted cell apoptosis in a dose-dependent manner. The effects of a high dose of cbFeD on colon cancer cells were similar to those of oxaliplatin. In HCT116 and SW480 cells, cbFeD inhibited the expression of IκBα, LC3B-I/II and Beclin-1, and the results of western blot analysis and immunofluorescence showed that, in the cells treated with cbFeD, p65 gradually entered nuclei in a dose-dependent manner, and the expression of LC3B-II was gradually reduced. The results of the acridine orangestaining and electron microscopy demonstrated fewer autophagic vesicles in the high-dose cbFeD group and the oxaliplatin group. The high dose of cbFeD reversed the effect of pyrrolidine dithiocarbamate, a p65-inhibitor, on the expression of p65, LC3B-I, LC3B-II and Beclin-1, and on the production of autophagic vacuoles. The high dose of cbFeD and oxaliplatin also suppressed tumorigenicity in vivo. The results of the H&E and IHC staining confirmed the inhibition of autophagy (LC3 and Beclin-1) and activation of p65 by treatment with the high dose of cbFeD and oxaliplatin. Taken together, cbFeD exhibited an antitumor effect in colon cancer cells by inhibiting autophagy through activation of the NF-κB pathway. Therefore, cbFeD may be a promising Chinese herbal compound for development for use in cancer therapy.

Pectin Oligosaccharides Ameliorate Colon Cancer by Regulating Oxidative Stress- and Inflammation-Activated Signaling Pathways

“…POS may inactivate NF-κB and affects the level of its downstream genes [cyclin D1 ( 135 ), TNF-α ( 136 ), and IL-6 ( 115 )] have been tested in NF-κB signaling pathway. Some data show that POS are the most potent activators of NF-κB signaling (Figure 6 ) ( 137 ), whereas the activation of NF-κB signaling pathway will promote CC apoptosis ( 138 ).…”

CCN3 and DLL1 co-regulate osteogenic differentiation of mouse embryonic fibroblasts in a Hey1-dependent manner

“…Our previous studies have confirmed that Notch signaling promoted osteogenic differentiation of MSCs induced by BMPs (BMP2, 4, 6, 7, and 9) through BMP signaling pathway 22 – 24 . Mitogen-activated protein kinases (MAPKs) are key branch of non-Smad BMP pathway.…”

“…Since BMPs and Notch signaling pathway play an important role in osteogenic differentiation, and our previous studies have confirmed that BMP9 had a strong stimulative ability 43 , whereas Notch signaling alone exerted no effect but could significantly promote BMP9-induced osteogenic differentiation 24 during this process, we hypothesized that CCN3 plays an inhibitory role by affecting BMP9 and Notch signaling pathway. We first examined the effect of CCN3 on BMP9 expression.…”

“…Our previous research has confirmed that the Notch signal activated by canonical membrane protein ligand DLL1 can promote bone morphogenetic protein (BMP) (BMP2, 4, 6, 7, and 9) induced osteogenic differentiation of mesenchymal stem cells (MSCs) by affecting BMP signaling 22 – 24 . However, as a non-canonical secreted ligand of the Notch signaling pathway, how does CCN3 affect the osteogenic differentiation of MSCs?…”

Prognostic and Predictive Values of the Autophagy Signature in Colon Cancer

“…In recent years, many studies have investigated the autophagic pathways in order to develop new potential targeted therapies [7][8][9][10][11]. In addition, autophagic drugs tend to induce cell autophagic death (type II cell death) and cause colon cancer cell death [12][13][14][15]. For instance, in the phase 1/2 clinical trials, NVP-BEZ235 was observed to induce colon cancer cell apoptosis and autophagy simultaneously [16,17].…”