Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing

Yeoman, Richard R.; Wolf, Don P.; Lee, David M.

doi:10.1016/j.fertnstert.2004.11.036

Cited by 72 publications

(61 citation statements)

References 41 publications

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“…Lower concentrations of cryoprotectant are required in freezing media for slow cooling, which reduces the risk of toxic and osmotic damage to cells, but this is insufficient to prevent ice crystal formation jeopardizing cell survival during the freezing procedures (Vajta 2006). Vitrification, though still regarded as a relatively novel freezing method, has recently been reported as an effective alternative method for the cryopreservation of ovarian tissue in various species, including mouse (Wang et al 2009), rat (Deng et al 2009), pig (Gandolfi et al 2006), goat (Santos et al 2007), sheep (Al-aghbari & Menino 2002, Courbiere et al 2006, monkey (Yeoman et al 2005), and human (Keros et al 2009, Zhou et al 2010. It combines a high cooling rate with a high concentration of cryoprotectant in the vitrification media, which rapidly dehydrates the cells and prevents water from precipitating as ice (Vajta 2006).…”

Section: Introductionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary follicle with a diameter around 100-130 mm) were mechanically isolated from vitrified-warmed and fresh adult mouse ovarian tissues and cultured for 12 days followed by an ovulation induction protocol at the end of this period to initiate oocyte maturation. Oocytes were then released from these follicles, fertilized in vitro, and cultured to the blastocyst stage and vitrified. After storage in liquid nitrogen for 2 weeks, groups of vitrified blastocysts were warmed and transferred into pseudo-pregnant recipient females. Although most of the isolated mouse pre-antral follicles from fresh (79.4%) and vitrified (75.0%) ovarian tissues survived the 12-day in vitro culture period, significantly fewer mature oocytes developed from vitrifiedwarmed pre-antral follicles than from the fresh controls (62.2 vs 86.4%, P!0.05). No difference was observed in embryo cleavage rates between these two groups, but the proportion of embryos that developed into blastocysts in the vitrification group was only half that of the controls (24.2 vs 47.2%, P!0.05). Nevertheless, live births of healthy normal pups were achieved after transfer of vitrified blastocysts derived from both experimental groups. This study shows that successful production of healthy offspring using an in vitro follicle culture system is feasible, and suggests that this procedure could be used in cancer patients who wish to preserve their fertility using ovarian tissue cryopreservation.

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Section: Introductionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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“…Using this method, tissue samples are normally placed in a very small volume of vitrification solution and cooled on a sterile metal surface to maximize the cooling rate, and minimize the potential for contamination of samples by LN 2 . Measures of survival of vitrified ovarian tissue after cryopreservation have included morphological examination (histology), viability assessment by viability staining or development in vitro or in vivo culture (Yeoman et al 2005, Santos et al 2007, Aerts et al 2008. There are, however, limited data on the effects on the function of ovarian tissue vitrified using solid surface vitrification systems.…”

Section: Introductionmentioning

confidence: 99%

Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice

Wang¹,

Catt²,

Pangestu³

et al. 2009

Reproduction

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Ovarian tissue cryopreservation and transplantation can be used to preserve fertility for cancer patients. In this study, we assessed the viability and function of ovarian tissue from adult mice that was cryopreserved by solid surface vitrification or traditional slow-cooling using various in vitro and in vivo techniques, including allotransplantation, in vitro oocyte maturation, embryo culture in vitro, blastocyst cryopreservation, embryo transfer, and development. The importance of cumulus cells for oocyte maturation, fertilization, and embryo development was investigated. Graft recovery, follicle survival, and oocyte retrieval was similar in control, vitrified, and slow-cooled groups. High rates of oocyte maturation, cleavage, and blastocyst formation were achieved, with no significant differences between the control, vitrified or slow-cooled ovarian tissue grafts. The presence of cumulus cells was important for oocyte maturation, fertilization, and subsequent development. Cumulus-oocyte complexes with no surrounding cumulus cells (N-COCs) or with an incomplete layer (P-COCs) had significantly lower rates of oocyte maturation and blastocyst formation than cumulus-oocyte complexes with at least one complete layer of cumulus cells (F-COCs; maturation rate: 63, 78 vs 94%; blastocyst rate: 29, 49 vs 80%). Live births were achieved using vitrified blastocysts derived from oocytes taken from vitrified and slow-cooled ovarian tissue heterotypic allografts. Successful production of healthy offspring from these vitrified blastocysts suggests that this technique should be considered as a useful stage to pause in the assisted reproduction pathway. This provides an alternative protocol for restoring fertility and offering cancer patients a better indication of their chances of pregnancy and live birth.

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“…There is one study on ovarian cortex from macaques when vitrification (3.4 M GLY, 4.5 M EG) and slow freezing (1.5 M EG) were compared and evaluated whether post-thawing coculture on feeder cells (mouse fetal fibroblas t m o n o l a y e r ) w i t h a d d i t i o n o f f o l l i c l e stimulating hormone, insulin, transferrin and selenium, would increase viability (Yeoman et al, 2005). The post-thaw viability, as assessed by live-dead fluorescent staining, was comparable (around 70%) in the two groups, which was only marginally lower than the follicular viability of the fresh tissue (76%).…”

Section: Non-human Primatesmentioning

confidence: 99%

Review on Ovarian Cryopreservation in Large Animals and Non-Human Primates

Milenković¹,

Díaz-García²,

Brännström³

2012

Current Frontiers in Cryopreservation

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Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing

Cited by 72 publications

References 41 publications

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice

Review on Ovarian Cryopreservation in Large Animals and Non-Human Primates

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