Coccoid like forms (CLF) of Helicobacter pylori. Enzyme activity and antigenicity

Nilius, M.; Ströhle, Andrea; Bode, Günter; Malfertheiner, Peter

doi:10.1016/s0934-8840(11)80964-3

Cited by 63 publications

(69 citation statements)

References 19 publications

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“…Different chemicals as bismuth, as well as bile acids, antibiotics, temperature, nutritients, oxygen tension, starvation and aging are some examples [3,10,15,16,21,25].…”

Section: Resultsmentioning

confidence: 99%

“…Some antibiotics seem to create greater changes to the cell wall than bismuth salts and bile acids [25]. In vivo changes and transformation mechanisms are less well known.…”

Section: Resultsmentioning

confidence: 99%

“…The role of the coccoid form in pathogenesis of H. pylori-associated gastritis has been disputed. Some authors consider the coccoid form as a degenerative or dead form of H. pylori [ 16,21,25], while others consider it as a resting but still metabolic active form [2, 3,12,22,29,321. The aim of this study was to demonstrate the conversion of spiral form of H. pylon to coccoid forms, and to show both degenerative and intact subcellar morphology suggesting that some of the coccoid bacteria may be viable.…”

Section: Introductionmentioning

confidence: 99%

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Morphologic Conversion of Helicobacter Pylori from Spiral to Coccoid Form: Scanning (SEM) and Transmission Electron Microscopy (TEM) Suggest Viability

Wïllén¹,

Carlén²,

Wang³

et al. 2000

Upsala Journal of Medical Sciences

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Helicobacter pylon is a pathogen associated with type B gastritis, peptic ulcer disease, gastric atrophy, and stomach cancer. H. pylori exists in two morphological forms, spirals and coccoids. The latter has been described as viable but non-cultivable. The role of the coccoid form in the pathogenesis of gastric disease is disputed. Some authors consider the coccoid form to be a degenerative or dead form of H.pylori, while others consider it a resting but still metabolically active form.This study reports the conversion fiom spiral to coccoid form ultrastructurally. Dense material is accumulated in the periplasmic space, the spiral bacteria bend and the outer membrane is separated from the inner cell wall layer. Remodeling of inner structures takes place, ending in the coccoid form of the bacteria with preserved light polyphosphate areas. Reduction of surface takes place by production of surface membrane vesicles, which later are squeezed off. The finding of preserved subcellular structures +d intact double membranes in combination with degenerative forms suggests that some of the coccoids are viable. Scanning electron microscopy (SEM) demonstrates coccoid form of bacteria with slightly ruffled surfaces but no spiral forms.

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“…Different chemicals as bismuth, as well as bile acids, antibiotics, temperature, nutritients, oxygen tension, starvation and aging are some examples [3,10,15,16,21,25].…”

Section: Resultsmentioning

confidence: 99%

“…Some antibiotics seem to create greater changes to the cell wall than bismuth salts and bile acids [25]. In vivo changes and transformation mechanisms are less well known.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphologic Conversion of Helicobacter Pylori from Spiral to Coccoid Form: Scanning (SEM) and Transmission Electron Microscopy (TEM) Suggest Viability

Wïllén¹,

Carlén²,

Wang³

et al. 2000

Upsala Journal of Medical Sciences

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show abstract

“…Since H. pylori have the propensity for exhibiting 2 forms: spiral and coccoid. The spiral-shaped form is metabolically active while the coccoid shape is formed under stressful conditions (17,18). Some authors consider the coccoid form as dormant and viable (17,(19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

“…The spiral-shaped form is metabolically active while the coccoid shape is formed under stressful conditions (17,18). Some authors consider the coccoid form as dormant and viable (17,(19)(20)(21). Morphological change from spiral to coccoid makes the organism non-culturable (22).…”

Section: Introductionmentioning

confidence: 99%

Detection of Helicobacter pylori in Drinking Water by Loop-Mediated Isothermal Amplification

Abiri

Bagherabadi

Kashef

et al. 2017

Jundishapur J Microbiol

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Background: There should be a public environmental reservoir for Helicobacter pylori in the developing countries, such as Iran, due to their high infection rate of over 70%. Epidemiological findings revealed that water could be a possible source of H. pylori transmission. However, high prevalence of H. pylori in drinking water in Kermanshah, West of Iran, was detected in the authors' previously published study. The current study aims at designing a more accurate and rapid procedure to investigate the prevalence of Helicobacter species and cagA gene in drinking water samples in Kermanshah, from October to December 2012. Methods: In the current study, 60 tap water samples were obtained and specific polymerase chain reaction (PCR) targeted cagA and 16s rRNA was performed. A loop-mediated isothermal amplification (LAMP) targeted ureC gene was developed to accurately detect H. pylori in water samples. Results: The prevalence of ureC by PCR, ureC by LAMP and 16s rRNA by PCR were 26.67%, 38%, and 61.67%, respectively. Among 24 samples (40%), 1 of the 2 tests was positive. The prevalence of cagA gene among ureC positive, 16s rRNA positive and all samples were 18.75%, 13.51%, and 10%, respectively. Conclusions: Helicobacter pylori contamination in drinking water was considerably higher using LAMP compared with PCR. It is noteworthy that some H. pylori positive samples were also positive for Caga.

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Overexpression and Purification of Helicobacter pylori Flavodoxin and Induction of a Specific Antiserum in Rabbits

Paul

Bosch²,

Schäfer³

2001

Protein Expression and Purification

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Coccoid like forms (CLF) of Helicobacter pylori. Enzyme activity and antigenicity

Cited by 63 publications

References 19 publications

Morphologic Conversion of Helicobacter Pylori from Spiral to Coccoid Form: Scanning (SEM) and Transmission Electron Microscopy (TEM) Suggest Viability

Morphologic Conversion of Helicobacter Pylori from Spiral to Coccoid Form: Scanning (SEM) and Transmission Electron Microscopy (TEM) Suggest Viability

Detection of Helicobacter pylori in Drinking Water by Loop-Mediated Isothermal Amplification

Overexpression and Purification of Helicobacter pylori Flavodoxin and Induction of a Specific Antiserum in Rabbits

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