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Anderson, Dennis G.

doi:10.1021/a1990002v

Cited by 7 publications

(8 citation statements)

References 165 publications

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“…Acetonitrile-water mixtures containing TFA at acidic pH are suitable for the chromatography of peptide hormones with a wide range of acid-base properties [12,13,14,38].…”

Section: Lc-uv Analysismentioning

confidence: 99%

“…Analytical and preparative liquid chromatography (LC) have been routinely used for fingerprinting and/or purification of new peptide products [12,13,14]. Because of the complexity of crude synthetic products, the preparation of significant amounts of purified peptides and the impurity characterizations first require the assessment of specific analytical-scale separation conditions.…”

Section: Introductionmentioning

confidence: 99%

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Liquid chromatography?mass spectrometry approach for the characterization and purification of crude synthetic peptide hormones

Sanz‐Nebot

Benavente

Toro

et al. 2003

Analytical and Bioanalytical Chemistry

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In this study, conditions for the optimal separation by LC-UV and characterisation by LC-ES-MS of crude mixtures generated during SPPS of several peptide hormones are compiled. The linear solvation energy relationship (LSER) methodology has been used to predict the retention of the target peptides and their side products and then to develop a separation LC methodology with applicability on both the analytical and preparative scale. Identification of these side products by LC-ES-MS analysis has been made on the basis of their calculated molecular masses. This method may be regarded as a key tool for the optimisation of the synthetic procedures and for complying with regulatory agencies' requirements before commercialisation of a safe and effective peptide-based pharmaceutical drug.

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“…Acetonitrile-water mixtures containing TFA at acidic pH are suitable for the chromatography of peptide hormones with a wide range of acid-base properties [12,13,14,38].…”

Section: Lc-uv Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Liquid chromatography?mass spectrometry approach for the characterization and purification of crude synthetic peptide hormones

Sanz‐Nebot

Benavente

Toro

et al. 2003

Analytical and Bioanalytical Chemistry

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“…Nevertheless, most of the studies carried out have focused on the identification of the synthetic polymers used as biding medium of modern paints whereas studies focused on the identification of additives are scarce. An interesting overview of these applications has been provided by Anderson [17].…”

Section: Introductionmentioning

confidence: 99%

Characterization of additives of PVAc and acrylic waterborne dispersions and paints by analytical pyrolysis–GC–MS and pyrolysis–silylation–GC–MS

Silva

Doménech‐Carbó

Osete-Cortina

2015

Journal of Analytical and Applied Pyrolysis

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a b s t r a c tCommercial formulations of poly(vinyl acetate) (PVAc) and acrylic dispersions and paints commonly used by artists include a number of additives such as surfactants, coalescing agents, defoamers and thickeners, which are designed for improving shelf-life, as well as chemical and physical properties of the resulting product. Recent studies have shown that additives present in paints play an important role in the alteration processes undergone by the painting during ageing and further in cleaning tasks planed in conservation interventions. However, the identification of additives is a difficult task due to the elusive character of these substances present at low concentration in the paint.In this context, a four-step approach is proposed that includes analysis of paint samples together with analysis of their water extracted products by pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) and pyrolysis-silylation-gas chromatography-mass spectrometry (Py-silylation-GC-MS). This analytical strategy enables a better characterization of common additives present in commercial PVAc and acrylic paints and dispersions. In particular, the analysis of water soluble extracts, which are mainly composed by paint additives, avoids the interference of the major polymer pyrolizates. Experimental conditions concerning sample preparation and instrumental working conditions of both Py-GC techniques are optimized.Both acrylic and PVAc paints presented poly(ethylene oxide) (POE) type fragments dominating the background of their pyrograms, especially when derivatized by means of hexamethyldisilazane (HMDS). For the first time, additives such as alkyl sulfate and alkyl ether sulfate with C 10 and C 12 alkyl chains, poly(ethoxylate) fatty alcohol and octylphenyl poly(ethoxylate) surfactants were identified, as well as polyvinyl alcohol (PVOH) protective colloids, hydrophobically modified ethoxylated urethane (HEUR) thickeners an defoamers. Their major fragments and corresponding mass spectra are discussed.

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“…Several techniques have been used to investigate the process of exocytosis, including intracellular fluorescent probes [11], membrane capacitance measurements [12], fluorescence microscopy based on an ultraviolet (UV) laser and a charge-coupled device detector [13,14], mass spectrometry [15], capillary electrophoresis (CE) and capillary high performance liquid chromatography with laser-induced fluorescence (LIF) [16,17,18,19] and electrochemical detection [20,21,22,23]. CE is an ideal tool for trace-level transmitter analysis because of its rapid separation ability, high separation efficiency, small sample consumption and compatibility with biological environments [24].…”

Section: Introductionmentioning

confidence: 99%

Analysis of serotonin release from single neuron soma using capillary electrophoresis and laser-induced fluorescence with a pulsed deep-UV NeCu laser

Miao

Rubakhin

Sweedler

2003

Analytical and Bioanalytical Chemistry

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The use of capillary electrophoresis (CE) with laser-induced fluorescence excited by ultraviolet (UV) lasers in the range 200-300 nm has been restricted by the available wavelengths and expense of UV lasers. The integration of a NeCu deep UV laser operating at 248.6 nm with a single channel CE system with post-column sheath flow detection allows detection limits for serotonin and tryptophan of 3.9x10(-8) M and 4.5x10(-8) M respectively. Single cell analysis of serotonergic metacerebral cells from the sea slug Aplysia californica yields a value of 800+/-85 fmol of serotonin in each cell soma. For the first time, serotonin is directly detected in electrically stimulated release from single metacerebral cell soma, with approximately 4% of the serotonin contained in the soma released from a semi-intact preparation with a 2 min electrical stimulation.

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Coatings

Cited by 7 publications

References 165 publications

Liquid chromatography?mass spectrometry approach for the characterization and purification of crude synthetic peptide hormones

Liquid chromatography?mass spectrometry approach for the characterization and purification of crude synthetic peptide hormones

Characterization of additives of PVAc and acrylic waterborne dispersions and paints by analytical pyrolysis–GC–MS and pyrolysis–silylation–GC–MS

Analysis of serotonin release from single neuron soma using capillary electrophoresis and laser-induced fluorescence with a pulsed deep-UV NeCu laser

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