Coagulase-negative staphylococci from non-mastitic bovine mammary gland: characterization of<i>Staphylococcus chromogenes</i>and<i>Staphylococcus haemolyticus</i>by antibiotic susceptibility testing and pulsed-field gel electrophoresis

Pate, Mateja; Zdovc, Irena; Avberšek, Jana; Ocepek, M.; Pengov, A.; Podpečan, Ožbalt

doi:10.1017/s0022029911000811

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…Consequently, contagious transmission among cows seems to be an important route for new IMI caused by S. chromogenes. Another possible reason for the high prevalence of S. chromogenes is its resistance to antibiotics (44) and its ability to persist in the mammary gland. Taponen et al (45) reported that 54.5% of IMI caused by S. chromogenes persisted throughout lactation.…”

Section: Discussionmentioning

confidence: 99%

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

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bCoagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n ‫؍‬ 108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n ‫؍‬ 80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI.

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Section: Discussionmentioning

confidence: 99%

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

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“…This suggests that either the host immune response or differences in NAS must be taken into account when discussing bacterial virulence and commensalism. Interestingly, S. chromogenes is most frequently isolated from milk and skin (69), but not from other environmental sources, suggesting that it is likely host-adapted (49,62,(70)(71)(72). Literature suggests that this species is largely isolated from samples of bovine origin, although it can be isolated from the milk of other dairy ruminants including goats and dairy buffalo (73).…”

Section: Staphylococcus Chromogenes: the Dominant Nas Speciesmentioning

confidence: 99%

Non-aureus Staphylococci and Bovine Udder Health: Current Understanding and Knowledge Gaps

Buck

Naushad

et al. 2021

Front. Vet. Sci.

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Despite considerable efforts to control bovine mastitis and explain its causes, it remains the most costly and common disease of dairy cattle worldwide. The role and impact of non-aureus staphylococci (NAS) in udder health are not entirely understood. These Gram-positive bacteria have become the most frequently isolated group of bacteria in milk samples of dairy cows and are associated with (mild) clinical and subclinical mastitis. Different species and strains of NAS differ in their epidemiology, pathogenicity, virulence, ecology and host adaptation, and antimicrobial resistance profiles. They have distinct relationships with the microbiome composition of the udder and may also have protective effects against other mastitis pathogens. Some appear to persist on the skin and in the teat canal and udder, while others seem to be transient residents of the udder from the environment. Analyzing genotypic and phenotypic differences in individual species may also hold clues to why some appear more successful than others in colonizing the udder. Understanding species-level interactions within the microbiome and its interactions with host genetics will clarify the role of NAS in bovine mastitis and udder health.

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“…In PFGE, the total genomic DNA is restricted by endonuclease that cleaves infrequently, and the fragments are analyzed by gel-electrophoresis with alternating power and direction of the electric current. Analysis using PFGE enables simple, accurate and reproducible comparison of isolates for genetic relatedness (Tenover et al, 1995) and several literature reports have documented the use of PFGE in typing staphylococcal species from bovine udder (Mørk et al, 2012; Pate et al, 2012; Bexiga et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of coagulase-negativeStaphylococcusspecies isolated at different lactation stages from dairy cattle in the United States

Jenkins

Okello

Rossitto

et al. 2019

PeerJ

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Background Coagulase negative Staphylococcus (CNS) species are currently the most prevalent intra-mammary pathogens causing subclinical mastitis and occasional clinical mastitis or persistent infection in lactating dairy cattle. More than 10 CNS species have been identified, but they are generally managed as one group on most dairies in the United States. However, improved management decisions and treatment outcomes may be achieved with better understanding of the prevalent species, pathogenicity and strain diversity within and across dairies. Methodology A total of 604 CNS isolates were cultured from milk samples collected during a dry-cow treatment clinical trial conducted on 6 dairy herds in 4 states in the US. All the study cows were randomized to receive 1 of the 3 different intra-mammary antimicrobial infusions (Quatermaster, Spectramast DC or ToMorrow Dry Cow) at dry-off. Milk samples were collected at dry-off, calving (0–6 days in milk, DIM), post-calving (7–13 DIM) and at mastitis events within the first 100 DIM. The CNS isolates were identified to species level by partial sequencing of the rpoβ gene, and genetic relatedness within species was investigated by phylogenetic analysis of the pulse-field gel electrophoresis profiles of the isolates. Results The major CNS species identified were S. chromogenes (48.3%), S. haemolyticus (17.9%), S. simulans and S. epidermidis (each at 6.5%). Other CNS species identified at lower frequencies included S. hominis, S. auricularis, S. sciuri, S. spp KS-SP, S. capitis, S. cohnii, S. warneri, S. pasteuri, S. xylosus, S. hyicus, S. equorum, S. microti, S. rostri, S. gallinarum, S. saprophyticus and S. succinus. Phylogenetic analyses of the major species types demonstrated an association between genetic relatedness and epidemiological distributions of S. chromogenes, S. simulans, S. haemolyticus and S. auricularis. Additionally, identical strains of S. chromogenes and S. simulans were isolated from the same udder quarter of several cows at consecutive sample stages. The rest of the minor species had no deducible genetic-epidemiological link. Discussion The observed association between genetic and epidemiological distributions indicated animal-adapted nature of four CNS species, suggesting possible host-adapted and environmental transmission of these species. Multi-stage isolation of the same udder quarter strain was evidence for chronic intra-mammary infection. Conclusion The different CNS species and strains circulating on US dairy herds were genetically diverse. Four species identified were likely udder-adapted pathogens, 2 of which caused persistent infection. Our findings are important in guiding the design of effective mastitis control strategies.

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Coagulase-negative staphylococci from non-mastitic bovine mammary gland: characterization ofStaphylococcus chromogenesandStaphylococcus haemolyticusby antibiotic susceptibility testing and pulsed-field gel electrophoresis

Cited by 8 publications

References 27 publications

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Non-aureus Staphylococci and Bovine Udder Health: Current Understanding and Knowledge Gaps

Molecular epidemiology of coagulase-negativeStaphylococcusspecies isolated at different lactation stages from dairy cattle in the United States

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