Coagulase-Negative Staphylococcal Skin Carriage among Neonatal Intensive Care Unit Personnel: from Population to Infection

Hira, Vishal; Sluijter, Marcel; Goessens, W. H. F.; Ott, Alewijn; Groot, Ronald de; Hermans, Peter W. M.; Kornelisse, René F.

doi:10.1128/jcm.00967-10

Cited by 51 publications

(29 citation statements)

References 16 publications

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“…Furthermore, others have proposed that hospitalization constitutes the most important factor for the accumulation of a hospital flora of CoNS [4]. In this aspect the skin flora of hospital personnel might be an important cause of cross-contamination of CoNS, as has been demonstrated in neonatal intensive care units [28,29].…”

Section: Discussionmentioning

confidence: 99%

Alteration of the colonization pattern of coagulase-negative staphylococci in patients undergoing treatment for hematological malignancy

Ahlstrand

Persson

Tidefelt

et al. 2011

Eur J Clin Microbiol Infect Dis

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The aim was to prospectively describe the colonization pattern of coagulase-negative staphylococci (CoNS) and the relationship between colonizing and invasive CoNS isolates among patients undergoing treatment for hematological malignancy. Fourteen newly diagnosed patients were included with either multiple myeloma or acute leukemia. Patients were repeatedly sampled from nares, throat, axillae, and perineum, and the CoNS isolates obtained were phenotypically characterized together with blood isolates of CoNS using the PhenePlate system (PhP). During the treatment a gradual reduction in the heterogeneity of colonizing CoNS was observed as well as an inter-patient accumulation of phenotypically related and multi-drug-resistant CoNS. These clusters of CoNS persisted for 2-3 months after the end of therapy. Ten positive blood cultures of CoNS were obtained and in the majority of these cases CoNS of the same PhP type were found in superficial cultures collected prior to the blood culture sampling. In conclusion, the study shows that therapy for hematological malignancy is associated with a homogenization of colonizing CoNS isolates and that this acquired flora of CoNS is persistent several months after the end of therapy. Furthermore, the results suggest that the source of bloodstream infections of CoNS in hematological patients is colonizing CoNS of the skin and mucosa.

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Section: Discussionmentioning

confidence: 99%

Alteration of the colonization pattern of coagulase-negative staphylococci in patients undergoing treatment for hematological malignancy

Ahlstrand

Persson

Tidefelt

et al. 2011

Eur J Clin Microbiol Infect Dis

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“…Bloodstream isolates were frequently antibiotic resistant, similar to CoNS isolates from NICU personnel and from NICU sites (1,2). Previously, it was shown that the majority of CoNS causing sepsis among neonates can be found on the hands of NICU personnel (1). Since the incidence of antibiotic-resistant CoNS in the nonmedical population is low, it is generally assumed that neonates become colonized with antibiotic-sensitive CoNS after birth.…”

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confidence: 80%

Colonization Dynamics of Antibiotic-Resistant Coagulase-Negative Staphylococci in Neonates

et al. 2013

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c Coagulase-negative staphylococci (CoNS) isolated in neonatal late-onset sepsis are often antibiotic resistant. We analyzed CoNS from skin and feces of neonates during hospitalization. Antibiotic resistance of skin isolates increased during hospitalization, especially in Staphylococcus haemolyticus. Staphylococcus warneri showed low antibiotic resistance. Our data suggest that different CoNS species may play distinct roles in colonization. C oagulase-negative staphylococci (CoNS) are the most frequent cause of late-onset sepsis among newborn infants in neonatal intensive care units (NICU) worldwide. Bloodstream isolates were frequently antibiotic resistant, similar to CoNS isolates from NICU personnel and from NICU sites (1, 2). Previously, it was shown that the majority of CoNS causing sepsis among neonates can be found on the hands of NICU personnel (1). Since the incidence of antibiotic-resistant CoNS in the nonmedical population is low, it is generally assumed that neonates become colonized with antibiotic-sensitive CoNS after birth. It is, however, unknown how skin and gut colonization with resistant CoNS develops during NICU hospitalization. A better understanding of CoNS colonization dynamics may assist the development of preventive strategies, for example, improvement of hygienic measures. We therefore studied CoNS colonization dynamics in neonates, focusing on the development of antibiotic resistance. We investigated skin and intestinal colonization, as well as maternal CoNS colonization after birth.This study was performed from mid November 2006 to mid March 2007 at the NICU of Erasmus MC-Sophia Children's Hospital, Rotterdam, The Netherlands. All infants born at this hospital with a presumed hospitalization time of at least 7 days (gestational age of Յ30 weeks, birth weight of Յ1,500 g or other reason) were included in this study. Children who were discharged within 72 h were excluded.We performed a longitudinal study of skin and intestinal carriage of CoNS among neonates and their mothers. Samples were taken from all infants 24, 48, and 72 h (Ϯ4 h) and, if still hospitalized, 7, 14, and 21 days after birth. Samples were taken from the mothers only once in the first 3 days and 7, 14, and 21 days after delivery. Skin samples from infants were obtained by gently pressing the bottom of a foot on a phenol-mannitol agar (PMA) plate (5% NaCl). Intestinal samples were obtained by culturing feces on PMA plates. Samples from mothers were obtained by culture of the thumb, by pressing it on a PMA plate (1). Selection, culturing, storage, species determination by internal transcribed spacer PCR, antibiotic susceptibility determination, and statistical analysis were performed as previously described (1), with the exception that in view of the multiple tests performed, we set the limit of significance at P ϭ 0.01 (two-sided), instead of the conventional P ϭ 0.05.Forty-one infants were initially included in the study. One infant was then excluded after being discharged within 72 h. General characteristics are summa...

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“…This could be achieved through strict antiseptic measures for environment, staff, equipment, and minimizing invasive procedures [13]. These perfect control measures require proper epidemiological studies and tracing of infection [14]. The principal method of diagnosing sepsis is the isolation of causative organisms from blood cultures and antimicrobial susceptibility testing [15,16].…”

Section: Introductionmentioning

confidence: 99%

Healthcare Associated Infection in the Neonatal Intensive Care Unit of King Abdl Aziz Specialist Hospital, Taif, KSA

Alzahrani¹,

Eed²,

Alsulaimani³

et al. 2013

AID

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Healthcare-associated infection is a common problem of newborn in neonatal intensive care units. It results in high mortality rate and serious complications. The Aim: to assess the incidence, etiology and the mortality of healthcareassociated infections of patients in neonatal intensive care unit at King Abdl Aziz Specialist Hospital (KAASH), Taif, Kingdom of Saudi Arabia. Material and Methods: This is a retrospective study including 8033 neonates admitted to neonatal intensive care unit during period between April, 2006 and December, 2012. The health-care associated infection rate, mortality rate, causative organism and risk factors were studied. Results: The prevalence of health-care associated infection was found to be 6.03%; the mortality rate was 27.1%. The highest prevalence was among children with the birth weight below 1000 g. The most frequent causative pathogen was klebseilla spp, followed by other gram negative bacilli. Conclusion: The rate of healthcare-associated infections in neonatal intensive care unit at KAASH was relatively high. In addition, the mortality rate was observed to be high (27.1%) owing to the high virulence of the causative organisms.

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Coagulase-Negative Staphylococcal Skin Carriage among Neonatal Intensive Care Unit Personnel: from Population to Infection

Cited by 51 publications

References 16 publications

Alteration of the colonization pattern of coagulase-negative staphylococci in patients undergoing treatment for hematological malignancy

Alteration of the colonization pattern of coagulase-negative staphylococci in patients undergoing treatment for hematological malignancy

Colonization Dynamics of Antibiotic-Resistant Coagulase-Negative Staphylococci in Neonates

Healthcare Associated Infection in the Neonatal Intensive Care Unit of King Abdl Aziz Specialist Hospital, Taif, KSA

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