Coagulase gene polymorphism of Staphylococcus aureus isolated from goat mastitis in Brazilian dairy herds

Silva, E.R.; Boechat, Jorge Ubirajara Dias; Silva, N.

doi:10.1111/j.1472-765x.2005.01799.x

Cited by 36 publications

(39 citation statements)

References 15 publications

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“…Of the 27 isolates of Staphylococcus, 2 (7.1%) were found to be producers of coagulase, the main virulence marker for the genus, which is associated with cases of infectious mastitis in milk cows SILVA, 2005). The results observed are consistent with those of other studies demonstrating that the family Muscidae is able to carry both coagulasepositive and -negative Staphylococcus species (BOUAMAMAA et al, 2010).…”

Section: Discussionsupporting

confidence: 82%

Muscoid Diptera as potential vectors of bacterial agents on dairy farms in the northern region of Paraná, Brazil

et al. 2014

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The aim of this study was to determine the isolation frequency and microbial sensitivity profiles of Escherichia coli, Salmonella spp., and Staphylococcus spp. isolated from synanthropic flies captured in entomological traps placed near the milking parlors of 30 dairy farms located in Northern Paraná, Brazil. In total, 192 flies were captured, and the isolation frequencies of muscids (21/30 = 70%) and califorids (27/30 = 90%) were significantly higher than that of sarcophagids (7/30 = 23.3%). Bacteriological tests on the internal contents and external surfaces of the flies showed that E. coli was present only in muscids (14.3%) and caliphorids (33.3%). Salmonella spp. was isolated from 9.5% of the muscids, 7.4% of the caliphorids, and 14.29% of the sarcophagids. Staphylococcus spp. was isolated from 28.5% of the muscids and 29.6% of the caliphorids. E. coli isolation was more frequent in flies captured on farms at which domestic chickens were housed near the milking parlors (p = 0.031) and which did not use sugarcane for animal feed (p = 0.042). Two out of 27 (7.4%) Staphylococcus spp. strains presented a phenotype of coagulase enzyme production. Ceftriaxone, ciprofloxacin, enrofloxacin, and gentamicin were the most effective antibiotics against E. coli and Salmonella spp. Tetracycline was the least effective antibiotic against the isolates. The Staphylococcus spp. strains isolated did not show the phenotype of oxacillin resistance. We conclude that regional flies are potential mechanical vectors of microbial agents able to cause enteritis in goats, mastitis in cows, and contamination of milk products for human consumption. Key words: Synanthropic flies, dairy cattle, and infectious agents ResumoMoscas sinantrópicas são vetores mecânicos de enfermidades infecciosas para humanos e animais. O presente estudo pretendeu avaliar a presença de Escherichia coli, Salmonella e Staphylococcus spp na população de moscas das famílias Calliphoridae, Muscidae e Sarcophagidae capturadas em 30 fazendas de leite na região Norte do Paraná. As capturas foram realizadas com armadilhas entomológicas contendo iscas a base de fígado bovino, penduradas em árvores próximas as salas de ordenhas das fazendas. Lavados da superfície externa e fluídos extraídos do abdome esmagado das moscas foram semeados em agar sangue ovino a 5%, agar Mac Conkey e agar Baird-Parker e submetidos a enriquecimento seletivo para Salmonella em caldo Rappaport-Vassiliadis e tetrationato Muller-Kauffmann, seguido de (33,3%). Salmonella foi isolada de 9,5% dos muscídeos, 7,4% dos califorídeos e de 14,29% dos sarcofagídeos capturados. Staphylococcus spp foi isolado de 28,5% dos muscídeos e 29,6% dos califorídeos. Os isolamentos de E. coli foram mais comuns e moscas capturadas em fazendas que mantinham criações de aves próximas ao local (p = 0,031) de ordenha e que não utilizavam cana-de-açúcar para alimentação dos animais (p=0,042). Conclui-se que as moscas presentes em fazendas de leite da região apresentam grande importância sanitária, visto que os agent...

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Section: Discussionsupporting

confidence: 82%

Muscoid Diptera as potential vectors of bacterial agents on dairy farms in the northern region of Paraná, Brazil

et al. 2014

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“…A caracterização fenotípica foi realizada por meio de testes de triagem que incluíram a morfologia macroscópica e microscó-pica, testes de catalase, oxidase, coagulase e produção de acetoína (teste de Voges Proskauer), realizados e interpretados segundo o National Mastitis Council (NMC, 2004). Isolados fenotipicamente identificados como S. aureus foram submetidos à identificação genotípica por meio da Reação em Cadeia da Polimerase (PCR) espécie-específica para o gene femA de S. aureus, de acordo com Silva;Silva (2005).…”

Section: Introductionunclassified

Resistência a antimicrobianos em Staphylococcus aureus isolados de mastite em bovinos leiteiros de Minas Gerais, Brasil

et al. 2013

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Staphylococcus aureus (S. aureus) destaca-se como um dos agentes etiológicos mais frequentes da mastite bovina, que causa os maiores prejuízos econômicos à pecuária leiteira brasileira. Visando avaliar o perfil de sensibilidade deste agente aos antimicrobianos, 352 isolados provenientes de 35 rebanhos leiteiros localizados em Minas Gerais foram submetidos a testes de antibiograma, utilizando-se a técnica de difusão em disco. Nitrofurantoína, bem como as associações de neomicina, bacitracina e tetraciclina (NBT) e penicilina, nafcilina e dihidroestreptomicina (PND), apresentaram 100% de eficiência in vitro. Foram verificados baixos índices de resistência no grupo das cefalosporinas, com valores de 0, 0,28 e 0,40% para cefquimona, cefalotina e ceftiofur, respectivamente. Entre os aminoglicosídeos, observou-se 1,69% de resistência para gentamicina e 3,35% para a neomicina. O maior índice de resistência foi observado para polimixina B (82%), seguido pelos betalactâmicos, ampicilina e penicilina, com índices de resistência de 80,92 e 80,45%, respectivamente. Níveis intermediários de resistência foram observados para tetraciclina, lincomicina, cefoperazona e sulfazotrim. Entre os isolados testados, 65 (18,15%), oriundos de 24 dentre os 35 rebanhos estudados, apresentaram multirresistência (índice MAR ≥ 0,2). Os resultados apontaram grande variação nos perfis de resistência aos antimicrobianos, assim como a ocorrência de múltipla resistência entre algumas cepas estudadas, salientando a necessidade de testes de antibiograma para a escolha dos antimicrobianos mais adequados para o tratamento ou prevenção de mastite causada por S. aureus.

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“…They determined that coagulase gene polymorphism of S. aureus isolates from mastitic cow milk by RFLP method using AluI enzyme produced 9 different genotype strains. Rodrigues da Silva and Silva [22] , reported that there were 49 different types of RFLP samples after digestion with AluI enzyme. Raimundo et al [23] examined coa gene type of 151 samples of cattle S. aureus isolates from 76 farms I II III IV I II III IV I II III IV I II III IV P 6 33 by using Coag2 and Coag3 primers and found 6 types of PCR.…”

Section: Discussionmentioning

confidence: 99%

“…It is reported that both counts and localisations of AluI restriction regions in 3'ends of gene coagulase contain a sequence of 81 base pairs which is different between S. aureus isolates [18] . The classification of S. aureus isolates depending on gene coa is a simple method for molecular typing and can be assumed as a validation test [18,22] . Aslantas et al [9] reported that RFLP models of gene coagulase of S. aureus isolates from cattle mastitis showed a great diversity.…”

Section: Discussionmentioning

confidence: 99%

Subklinik Mastitli İneklerden İzole Edilen Staphylococcus aureus İzolatlarinin Antibiyotik Duyarlılık Profillerinin Çıkarılması ve Koagulaz Geni Polimorfizmine Göre Tiplendirilmesi

Kenar¹,

Bağcıgil²,

Kuyucuoğlu³

et al. 2017

Kafkas Univ Vet Fak Derg

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The purpose of the study was to isolate Staphylococcus aureus from bovine subclinical mastitis, determine their antibiotic susceptibilities and investigate the coagulase gene polymorphism by using a PCR-based restriction fragment length polymorphism (RFLP) method. Milk samples from 463 CMT positive udders from 237 cows cultured. The antimicrobial susceptibility of the isolates were determined by disc diffusion method. A total of 82 out of the 83 isolates (98.8%) were found to be resistant at least one out of the 16 antibiotics studied. In this experiment 53 isolates (63.8%) were found to be resistant to penicillin; 52 (62.67%) to trimethoprim/sulphamethoxazole; 51 (61.5%) to ampicillin; 40 (48.2%) to erytromycin; 29 (34.9%) to tetracycline; 18 (21.6%) to ciprofloxacin, 16 (19.3%) to clindamycin, 13 (15.6%) to chloramphenicol; 8 (9.6%) to gentamicin; 5 (6.0%) to cefoxitin; 4 (4.9%) to vancomycin; 3 (3.6%) to cephalotin; 2 (2.4%) nafcillin; one (1.2%) to oxacillin and one to (1.2%) furazolidon. No imipenem resistance was seen in the S. aureus isolates. The coagulase gen polymorphism were examined by PCR amplification of coagulase gene followed by AluI digestion of repeating 81 bp DNA sequences. After nested PCR, double bands were produced in 8 of the isolates while there were single band in remaining 75 isolates. Following AluI digestion, isolates that formed single band in length of approximately 300 bp showed 3 different groups. Keywords: Bovine subclinical mastitis, Staphylococcus aureus, Antibiotic susceptibility, Coagulase gene polymorphism Subklinik Mastitli İneklerden İzole Edilen Staphylococcus aureus İzolatlarinin Antibiyotik Duyarlılık Profillerinin Çıkarılması ve Koagulaz Geni Polimorfizmine Göre Tiplendirilmesi ÖzetBu çalışmanın amacı subklinik mastitisli sığırlardan Staphylococcus aureus'u izole etmek, bunların antibiyotiklere duyarlılığını belirlemek ve bir PCR tabanlı restriksiyon fragment lengh polimorfizmi (RFLP) yöntemi kullanarak koagulaz gen polimorfizmi araştırmaktır. 463 sığırdan CMT pozitif olan 237 sığır memesinden süt örnekleri alınarak ekim yapılmıştır. İzolatların antimikrobiyal duyarlılığı disk difüzyon yöntemi ile belirlenmiştir. Toplam 83 izolatın 82'si (%98.8) uygulanan 16 antibiyotikten en az bir antibiyotiğe dirençli bulundu. Bu çalışmada 53 izolat (%63.8) penisiline, 52 izolat (%62.67 trimethoprim/sulphamethoxazole, 51 izolat (%61.5) ampisiline, 40 izolat (%48.2) eritromisine, 29 izolat (%35.0) tetrasikline, 18 izolat (%21.7) siprofloksasine, 16 izolat (%19.3) klindamisine, 13 izolat (%15.6) kloramfenikole, 8 izolat (%9.6) gentamisine, 5 izolat (%6.0) sefoksitine, 4 izolat (%4.9) vankomisine, 3 izolat (%3.6) sefalotine, 2 izolat (%2.4) nafsiline ve 1'er (%1.2) izolat ise oksasilin ve furazolidona dirençli bulundu. S. aureus izolatlarında imipenem dirençliliği görülmedi. Koagulaz gen polimorfizmi koagulaz genin tekrarlanan 81 bp DNA dizisinin AluI sindirimini müteakiben koagulaz genin amplifikasyonu ile incelenmiştir. Nested PCR'den sonra izolatların 8'inde çift bant görülm...

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Coagulase gene polymorphism of Staphylococcus aureus isolated from goat mastitis in Brazilian dairy herds

Cited by 36 publications

References 15 publications

Muscoid Diptera as potential vectors of bacterial agents on dairy farms in the northern region of Paraná, Brazil

Muscoid Diptera as potential vectors of bacterial agents on dairy farms in the northern region of Paraná, Brazil

Resistência a antimicrobianos em Staphylococcus aureus isolados de mastite em bovinos leiteiros de Minas Gerais, Brasil

Subklinik Mastitli İneklerden İzole Edilen Staphylococcus aureus İzolatlarinin Antibiyotik Duyarlılık Profillerinin Çıkarılması ve Koagulaz Geni Polimorfizmine Göre Tiplendirilmesi

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