Co-ordinated gene expression during phases of dormancy release in raspberry (Rubus idaeus L.) buds

Abstract

Bud break in raspberry (Rubus idaeus L.) is often poor and uneven, with many of the subapical buds remaining in a dormant state. In order to determine the dormancy status of raspberry buds, an empirical measure of bud burst in a growth-permissive environment following exposure to chilling (4 degrees C cold storage) was developed. For cv. Glen Ample, percentage bud burst in intact canes and isolated nodes was recorded after 14 d. Isolated nodes (a measure of endodormancy) achieved 100% bud burst after approximately 1500 h chilling whereas buds on intact plants (combined endo- and paradormancy) required an additional 1000 h chilling. A microarray approach was used to follow changes in gene expression that occurred during dormancy transition. The probes for the microarrays were obtained from endodormant and paradormant raspberry bud cDNA libraries. The expression profiles of 5300 clones from these libraries were subjected to principal component analysis to determine the most significant expression patterns. Sequence analysis of these clones, in many cases, enabled their functional categorization and the development of hypotheses concerning the mechanisms of bud dormancy release. Thus a set of novel candidates for key dormancy-related genes from raspberry buds have been identified. Bud dormancy is fundamental to the study of plant developmental processes and, in addition, its regulation is of significant economic importance to fruit and horticultural industries.

Regulation of Bud Dormancy and Bud Break in Japanese Apricot (Prunus mume Siebold ^|^amp; Zucc.) and Peach [Prunus persica (L.) Batsch]: A Summary of Recent Studies

“…Negative correlation of peach PpDAM5 and PpDAM6 expression with the time required for bud break was also reported for lateral vegetative (Yamane et al, 2011a) and flower (Yamane et al, 2011b, c) buds. In other temperate fruit trees, down-regulation of the SVP-like gene during dormancy release has been reported in raspberry (Rubus idaeus L.) (Mazzitelli et al, 2007). In Japanese pear, the expression of the DAM-like gene MADS13 was upregulated towards dormancy establishment and downregulated towards dormancy release .…”

“…Examples of recently published omics studies using agronomically important fruit tree species include: grapevine (Vitis spp.) (Diaz-Riquelme et al, 2012;Mathiason et al, 2009); Japanese pear (Pyrus pyrifolia) Liu et al, 2012;Nishitani et al, 2012); chestnut (Castanea sativa) (Santamaría et al, 2011); raspberry (Rubus idaeus) (Mazzitelli et al, 2007); blackcurrant (Ribes nigrum L.) (Hedley et al, 2010); and Prunus spp. (described in detail below).…”

Comparative Analyses of Dormancy-associated MADS-box Genes, PpDAM5 and PpDAM6, in Low- and High-chill Peaches (Prunus persica L.)

“…et Zucc.) (Yamane et al, 2008) and raspberry (Mazzitelli et al, 2007) are also associated with lateral bud endodormancy release. The recent finding of the co-localization of a strong QTL for CR and blooming time within the region of the EVG locus where PpDAM5 and PpDAM6 reside (Fan et al, 2010) further supports the idea that PpDAM5 and PpDAM6 could control CR of peach bud.…”

454-Pyrosequencing of the Transcriptome in Leaf and Flower Buds of Japanese Apricot (Prunus mume Sieb. et Zucc.) at Different Dormant Stages

“…(Derory et al, 2006), poplar (Populus spp.) (Ko et al, 2011;Ruttink et al, 2007), raspberry (Rubus idaeus L.) (Mazzitelli et al, 2007), grapevine (Vitis riparia Michx.) (Mathiason et al, 2009), blackcurrant (Ribes nigrum L.) (Hedley et al, 2010), and leafy spurge (Euphobia esula L.) (Doğramaci et al, 2010).…”