Co-Occurrence of the West European (Gr.III) and North American (Gr.I) Ribotypes of Alexandrium tamarense (Dinophyceae) in Shetland, Scotland

Touzet, Nicolas; Davidson, Keith; Pete, Romain; Flanagan, Kalen; McCoy, G. F.; Amzil, Zouher; Maher, Majella; Chapelle, Annie; Raine, Robin

doi:10.1016/j.protis.2009.12.001

Cited by 42 publications

(44 citation statements)

References 47 publications

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“…Most research effort in the region has concentrated on biotoxin producing species of Alexandrium (Collins et al, 2009;Touzet et al 2010;Eckford-Soper et al, 2013), Pseudo-nitzschia (Fehling et al, 2004a(Fehling et al, , 2005clusters of organisms. The particles are typically imbued with aspects of the organism's biology (e.g.…”

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confidence: 99%

Individual-based modelling of the development and transport of a Karenia mikimotoi bloom on the North-west European continental shelf

et al. 2016

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Individual-based modelling of the development and transport of a Karenia mikimotoi bloom on the North-west European continental shelf

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“…As it is often difficult to distinguish between phytoplankton species or strains of interest and other morphologically similar organisms by light microscopy alone, identification using molecular markers is increasingly common (Touzet et al 2010). Species or strain specific FISH probes used in combination with methods that characterize cells, such as microscopy or flow cytometry, can therefore be useful tools for evaluating individual phytoplankton species or strains.…”

Section: Discussionmentioning

confidence: 99%

“…Detection and discrimination of toxic and Table 1. Taxa-specific oligonucleotide probes TamA (Group III) and TamToxC (Group I) targeting sites in the D1-D2 domain of LSU rDNA (Touzet et al 2010). where C is the cell incidences count within the gated regions (R2 and R3) of Fig.…”

Section: Fc Enumeration Of Ribotypesmentioning

confidence: 99%

Identification and quantification of toxic and nontoxic strains of the harmful dinoflagellate Alexandrium tamarense using fluorescence in situ hybridization and flow cytometry

Eckford-Soper

Davidson

Bresnan

2013

Limnology & Ocean Methods

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The co-occurrence of morphologically identical toxic and nontoxic ribotypes of the biotoxin producing marine dinoflagellate Alexandrium tamarense presents a significant problem for its identification and enumeration, particularly in a regulatory monitoring context. To address this, we have developed a fluorescence in situ hybridization-flow cytometry (FISH-FC)-based method of cell identification and enumeration. This employed the taxa specific oligonucleotide probes TamToxC and TamA to fluorescently label (with the fluorochromes CY.3 and FITC) Group I (toxic) and Group III (nontoxic) A. tamarense ribotypes, respectively. Detection was by fluorescence activated flow cytometric analysis. The FISH-FC method allowed effective discrimination between laboratory cultures of Group I and Group III ribotypes, with toxic and nontoxic cells creating distinct, easily identifiable, clusters in a flow cytometer bi-plot of side scatter (SSC) versus the green (FL1) fluorescence detection channel. Comparison of estimates of cell abundance obtained by the FISH-FC technique with those obtained by microscopy (Sedgwick Rafter technique) showed no statistically significant difference across a range of concentrations. Subsequently, the methodology was successfully applied on natural seawater samples, spiked with known concentrations of toxic and nontoxic A. tamarense cells at environmentally relevant concentrations. AcknowledgmentsThis work was funded by a NERC (CASE) studentship to LES at SAMS/UHI. KD was part funded by the Food Standards Agency (project FS241058), the EU FP7 project Asimuth, and the Culture Collection for Algae and Protozoa (CCAP). We thank the crew of the RV Seol Mara for their help with water collection. David Green, Sharon McNeill, Elaine Mitchell, and Mark Hart (SAMS) for their experience and expertise and Jean-Pierre Lacaze (Marine Scotland Science) for useful discussions.

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“…In the UK waters there are several species responsible for the production of biotoxins. In Scotland, shellfish contamination with biotoxins produced by harmful algal species belonging to Dinophysis, Pseudo-nitzschia and Alexandrium occur annually (Touzet et al, 2010). Several Pseudo-nitzschia species are found frequently in Scottish waters including P. delicatissima, P. calliantha, P. pseudodelicatissima.…”

Section: Introductionmentioning

confidence: 99%

Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within European waters: A comparison of methods of analysis

et al. 2016

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Highlights Use of a multiplex biosensor for harmful algal toxin monitoring efforts in Europe. Toxin and species data compared for seawater samples (n= 256). Biosensor and RNA microarray more sensitive than light microscopy. Capability of being employed as an early warning detection system.  Advanced solutions to algal biotoxin monitoring. AbstractHarmful algal blooms (HABs) are a natural global phenomena emerging in severity and extent. -74% for all three toxin families illustrating that one individual testing method may not be an ideal solution. An efficient early warning monitoring system for the detection of toxic HABs could therefore be achieved by combining both the multitoxin SPR biosensor and RNA microarray.

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Co-Occurrence of the West European (Gr.III) and North American (Gr.I) Ribotypes of Alexandrium tamarense (Dinophyceae) in Shetland, Scotland

Cited by 42 publications

References 47 publications

Individual-based modelling of the development and transport of a Karenia mikimotoi bloom on the North-west European continental shelf

Individual-based modelling of the development and transport of a Karenia mikimotoi bloom on the North-west European continental shelf

Identification and quantification of toxic and nontoxic strains of the harmful dinoflagellate Alexandrium tamarense using fluorescence in situ hybridization and flow cytometry

Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within European waters: A comparison of methods of analysis

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