Co-Occurrence of Multidrug Resistant Klebsiella pneumoniae Pathogenic Clones of Human Relevance in an Equine Pneumonia Case

Venturini, Carola; Bowring, Bethany; Partridge, Sally R.; Zakour, Nouri L. Ben; Fajardo-Lubián, Alicia; Ayala, Ariana Lopez; Qin, Jilong; Totsika, Makrina; Galen, Gaby Van; Norris, Jacqueline M.; Iredell, Jonathan R.

doi:10.1128/spectrum.02158-21

Cited by 6 publications

(4 citation statements)

References 63 publications

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“…For example, this study did not use a pre-enrichment step for ESBL- and AmpC-producing E. coli prior to plating on selective agar, which is a technique used in some prevalence studies ( Hordijk et al, 2013 ; Gonggrijp et al, 2016 ; Hutchinson et al, 2017 ). In addition, selecting one isolate per sample may not be sufficient to reflect the bacterial heterogeneity associated with an ecological niche ( Venturini et al, 2022 ), therefore, analysing multiple isolates per sample is beneficial in prevalence studies. The age of the study population is also a crucial factor to consider.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and distribution of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in two New Zealand dairy farm environments

et al. 2022

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Antimicrobial resistance (AMR) is a global threat to human and animal health, with the misuse and overuse of antimicrobials being suggested as the main driver of resistance. In a global context, New Zealand (NZ) is a relatively low user of antimicrobials in animal production. However, the role antimicrobial usage on pasture-based dairy farms, such as those in NZ, plays in driving the spread of AMR within the dairy farm environment remains equivocal. Culture-based methods were used to determine the prevalence and distribution of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli from farm environmental samples collected over a 15-month period from two NZ dairy farms with contrasting management practices. Whole genome sequencing was utilised to understand the genomic epidemiology and antimicrobial resistance gene repertoire of a subset of third-generation cephalosporin resistant E. coli isolated in this study. There was a low sample level prevalence of ESBL-producing E. coli (faeces 1.7%; farm dairy effluent, 6.7% from Dairy 4 and none from Dairy 1) but AmpC-producing E. coli were more frequently isolated across both farms (faeces 3.3% and 8.3%; farm dairy effluent 38.4%, 6.7% from Dairy 1 and Dairy 4, respectively). ESBL- and AmpC-producing E. coli were isolated from faeces and farm dairy effluent in spring and summer, during months with varying levels of antimicrobial use, but no ESBL- or AmpC-producing E. coli were isolated from bulk tank milk or soil from recently grazed paddocks. Hybrid assemblies using short- and long-read sequence data from a subset of ESBL- and AmpC-producing E. coli enabled the assembly and annotation of nine plasmids from six E. coli, including one plasmid co-harbouring 12 antimicrobial resistance genes. ESBL-producing E. coli were infrequently identified from faeces and farm dairy effluent on the two NZ dairy farms, suggesting they are present at a low prevalence on these farms. Plasmids harbouring several antimicrobial resistance genes were identified, and bacteria carrying such plasmids are a concern for both animal and public health. AMR is a burden for human, animal and environmental health and requires a holistic “One Health” approach to address.

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Section: Discussionmentioning

confidence: 99%

Prevalence and distribution of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in two New Zealand dairy farm environments

et al. 2022

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“…Another severe disease reported in horses is pneumonia caused by MDR isolates of Klebsiella pneumoniae, a putative zoonotic pathogen [36]. It was recently demonstrated that multiple clones of K. pneumoniae carrying different antibiotic resistance genes can be copresent in a single episode of infection, with each clone being sensitive to the lytic activity of different bacteriophages [37]. This study, which emphasizes the use of bacteriophage typing as part of routine testing, is a basis for the identification and characterization of new phages with lytic activity that might be considered for future application against MDR K. pneumoniae.…”

Section: Horsesmentioning

confidence: 99%

Bacteriophage Therapy in Companion and Farm Animals

Bianchessi,

De Bernardi,

Vigorelli

et al. 2024

Antibiotics

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Bacteriophages, which are viruses with restricted tropism for bacteria, have been employed for over a century as antimicrobial agents; they have been largely abandoned in Western countries but are constantly used in Eastern European countries with the advent of antibiotics. In recent decades, the growing spread of multidrug-resistant bacteria, which pose a serious threat to worldwide public health, imposed an urgent demand for alternative therapeutic approaches to antibiotics in animal and human fields. Based on this requirement, numerous studies have been published on developing and testing bacteriophage-based therapy. Overall, the literature largely supports the potential of this perspective but also highlights the need for additional research as the current standards are inadequate to receive approval from regulatory authorities. This review aims to update and critically revise the current knowledge on the application of bacteriophages to treat bacterial-derived infectious diseases in animals in order to provide topical perspectives and innovative advances.

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“…Inhibiting these genes or proteins could potentially restore sensitivity to antibiotics in multidrug-resistant strains. 59 Recently, Venturini et al 60 focused on these genes and suggested clinicians can use them as diagnostic markers to identify multidrug-resistant strains of KP and PA, enabling informed decisions on selecting appropriate antibiotic treatments for better patient outcomes. In personalized treatment strategies, some of the MDR genes also contribute to allowing tailored treatment based on the Gene Expr genetic characteristics of the infecting strain, thereby optimizing patient outcomes.…”

Section: Go and Pathway Enrichment Analysismentioning

confidence: 99%

Identification and Exploration of Transcripts Involved in Antibiotic Resistance Mechanism of Two Critical Superbugs

Sahoo,

Mohanty,

Routray

et al. 2024

Gene Expr

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Background and objectives: Infectious diseases caused by pathogenic strains of bacteria are a global cause of morbidity and mortality. Hospital-acquired infections caused by Klebsiella pneumonia and Pseudomonas aeruginosa were found vulnerable during the COVID-19 pandemic. They are also responsible for the onset of certain life-threatening infectious diseases such as cystic fibrosis, endocarditis, bacteremia, and sepsis. Looking into the importance of these two superbugs there is a strong need for extensive comparative differential gene expression analysis among the wild-type and mutant for betterment of intensive care unit patients especially as such pathogenic bacterial strains have a dangerous role in the intensive care unit.Methods: This study revealed the RNA microarray gene expression profiles of GSE24688, GSE4026, and GSE117438. The study compared all genes from three different datasets and all drug resistance genes from two divergent organisms, Klebsiella pneumonia and Pseudomonas aeruginosa.Results: 10 numbers of shared significant genes and five drug resistance genes were obtained in this study. These putative genes may show intriguing patterns of connection with resistance mechanisms and can be used in the field of diagnostics and treatment. Our divergent analysis also revealed a very clear distinct relation between Klebsiella pneumoniae and Pseudomonas aeruginosa at the genetic level, though they both function under antimicrobial resistance. Conclusions:This study enhances the understanding of the genetic basis, providing valuable knowledge for the development of new strategies to combat antibiotic resistance and enhance the efficacy of existing antibiotics.

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Co-Occurrence of Multidrug Resistant Klebsiella pneumoniae Pathogenic Clones of Human Relevance in an Equine Pneumonia Case

Abstract: Multidrug resistant Klebsiella pneumoniae are agents of life-threatening infections in animals and humans, with several multidrug resistant clones causing outbreaks of disease worldwide. It is generally accepted that only one clone will be dominant in an infection episode.

Cited by 6 publications

References 63 publications

Prevalence and distribution of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in two New Zealand dairy farm environments

Prevalence and distribution of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in two New Zealand dairy farm environments

Bacteriophage Therapy in Companion and Farm Animals

Identification and Exploration of Transcripts Involved in Antibiotic Resistance Mechanism of Two Critical Superbugs

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