Co-existence of blaNDM-1 and blaVIM-1 producing Moellerella wisconsensis in NICU of North Indian Hospital

Abstract

Infections caused by carbapenemase-producing Enterobacteriaceae have become a major threat to public health, worldwide. Here we report clinically significant NDM-1 and VIM-1 producing Moellerella wisconsensis which has not yet been described in the literature; this is the first report of M. wisconsensis strain harbouring blaNDM-1 and blaVIM-1, recovered from the rectal swab of a low birth weight female child admitted in NICU of the north Indian tertiary care hospital. A plasmid of IncW incompatibility with size of 154 kb was observed in AK-92 strain.

Efficacy of Novel Combinations of Antibiotics against Multidrug-Resistant—New Delhi Metallo-Beta-Lactamase-Producing Strains of Enterobacterales

“…These strains were obtained from NICU of a North Indian Hospital. These are ESBL-and MBL-producing strains with different resistant markers, as reported previously by our group [7][8][9][10][11][12][13]. The antibiotic resistance markers and MIC of these strains are presented in Table 1.…”

“…The MICs of cefoxitin, doripenem, imipenem, and streptomycin against MDR strains carrying blaNDM and other associated resistance markers on a plasmid, were reported in the range between 4096 µg/mL and 128 µg/mL (Table 1). All these MDR strains have already been characterized for the presence of resistance markers in our laboratory, and these isolates were collected from the neonatal intensive care unit (NICU) of an Indian hospital [7][8][9][10][11][12][13].…”

Microbiota Survey of Sliced Cooked Ham During the Secondary Shelf Life

“…This species is probably an environmental contaminant, as literature reports a variety of isolation sources, including water, foods, and samples of human origin ( Leroy et al, 2016 ). M. wisconsensis can be involved in the dissemination of antibiotic resistance, and its pathogenic role is debated ( Stock et al, 2003 ; Leroy et al, 2016 ; Ahmad et al, 2020 ).…”

Infections Caused by Moellerella wisconsensis: A Case Report and a Systematic Review of the Literature

“…Although it is an uncommon bacterium in the daily clinical practice, there are several case reports in the literature describing its isolation from clinical specimens, such as human stool, bile, blood, bronchial aspirate, and wound swab, and its relationship with clinically overt disease [ 1 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 21 ]. Regarding our report, it represents the fourth case of isolation of M. wisconsensis from blood culture and the first case of its isolation from pigtail end and urine cultures.…”

“…However, acquired resistance of this bacterium against several classes of antibiotics may emerge, and multi-drug or extended-drug resistant strains have been isolated in many cases, including the case reported here. The most worrisome of all is that there has been already described cases of infections with M. wisconsensis strains that harbored plasmids containing genes that conferred resistance to carbapenems, such as blaNDM-1 and blaVIM-1 [ 19 ], and these plasmids could be transferred easily between bacteria within this species. These genes confer resistance to almost all β-lactams, which is extremely alarming considering the intrinsic resistance of this bacterium to colistin.…”