Co-administration of ginseng and ciprofloxacin ameliorates epididymo-orchitis induced alterations in sperm quality and spermatogenic cells apoptosis following infection in rats

Abstract

Korean red ginseng (KRG) may be a beneficial adjuvant along with ciprofloxacin to ameliorate devastating effects of epididymo-orchitis (EO) on male fertility. This study intends to assay the effects of KRG and ciprofloxacin on sperm quality and spermatogenic cells apoptosis in EO rats. We divided 54 adult rats into nine groups (n = 6 rats per group): control (CO), sham-operated (SH), EO (E); ciprofloxacin (C), EO-ciprofloxacin (EC), KRG (G), EO-KRG (EG), ciprofloxacin-KRG (CG) and EO-ciprofloxacin-KRG (ECG). We administered ciprofloxacin and KRG 48 hr after the Escherichia coli (E. coli) injection for 10 days. Bilateral orchiectomy was performed after one sperm cycle (14 days) following the last treatment with ciprofloxacin and KRG. Total and progressive motility of E, C and EC groups decreased. However, motility is improved in CG and ECG in comparison with these groups. The E group induced negative changes in the architecture of testes tissue and dramatic increase in apoptosis indices. Interestingly, co-administration of ciprofloxacin and KRG has dramatically improved Miller's and Johnsen's scores and decreased the apoptosis indices of animals in the ECG group. Combined treatment of ciprofloxacin and KRG may improve the quality of spermatozoa and attenuated apoptosis indices in the ECG group.

Protective role of ginseng extract against oxidative stress, reproductive and some biochemical parameters alterations induced by doxorubicin in male rats

“…They found that ginseng induced non-significant elevation in total protein, albumin and globulin. Also, Eskandari et al (2017) found that ginseng induced non-significant elevation in total protein and globulin in rats. On the other hand, Doxorubicin plus ginseng group showed non-significant changes in total protein and albumin levels in comparison either with control group and doxorubicin group.…”

Ameliorating impacts of ginseng on the apoptosis of spermatogenic cells and sperm quality in temporal lobe epilepsy rat model treated with valproate

“…A number of studies showed that ginseng could promote sperm quality under different experimental conditions (Park et al, ). The protective effects of ginseng on sperm quality might refer to its antioxidant impact, ROS scavenging and increase in the antioxidative defence system (Eskandari et al, ). In addition, ginseng can induce sperm hyperactivation by increasing sperm cation channel (CatSper) gene expression (Park, Kim, Kim, Park, & Chang, ).…”

“…The stock solution consisted of 100 mg dried extract per ml of sterile phosphate‐buffered saline (PBS). The extract solution was sterilised by filtration through a 0.2 µ Millipore filter (Eskandari et al, , ) .…”

Ginseng for Improving Semen Quality Parameters: A Systematic Review

“…Several preclinical studies have reported that ginseng promotes spermatogenesis, as well as improving testicular problems, sperm quality, and sperm mobility, due to be a cyclic adenosine monophosphate-responsive element modulator [15][16][17][18][19][20]. Recent reviews summarize that ginsenosides-the predominant active compounds of ginseng-may affect estrogen and androgen activity, and act as an aphrodisiac [21,22].…”