Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor.

Hernández-Eugenio, Guadalupe; Fardeau, Marie‐Laure; Cayol, Jean‐Luc; Patel, Bharat K. C.; Thomas, Pierre; Macarie, Hervé; Garcia, Jean‐Louis; Ollivier, Bernard

doi:10.1099/00207713-52-5-1461

Cited by 27 publications

(9 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the sulfur metabolism capabilities of Clostridium subatlanticum have not been reported, strain Lup 21 T designated Clostridium thiosulfatireducens sp. nov. [51], and C. peptidivorans [52] have been shown to reduce thiosulfate to sul¢de. Therefore, in addition to an SRB partner coupling with an aceticlastic methanogen as has been reported for other cold seep habitats [23^25,27^29], we hypothesize that fermentative thiosulfate-reducing bacteria could thus potentially play an important role in the AOM in GoM hydrocarbon seep sediments.…”

Section: Phylogenetic Diversity Of Srbmentioning

confidence: 99%

Microbial diversity in sediments associated with surface-breaching gas hydrate mounds in the Gulf of Mexico

Mills

Hodges

Wilson

et al. 2003

FEMS Microbiology Ecology

125

111

View full text Add to dashboard Cite

A molecular phylogenetic approach was used to characterize the composition of microbial communities from two gas hydrate sedimentary systems in the Gulf of Mexico. Nucleic acids, extracted from sediments directly overlying surface-breaching gas hydrate mounds collected from a research submersible (water depth 550-575 m), were amplified with nine different 16S rDNA gene primer sets. The polymerase chain reaction primers targeted microorganisms at the domain-specific (Bacteria and Archaea) and group-specific (sulfate-reducing bacteria (SRB) and putative anaerobic methane-oxidizing (ANME) archaea) level. Amplicons were obtained with five of the nine primer sets including two of the six SRB Groups (SRB Group 5 and Group 6) and used to generate five different clone libraries. Analysis of 126 clones from the Archaea library revealed that the sediments associated with naturally occurring gas hydrate harbored a low diversity. Sequence analysis indicated the majority of archaeal clones were most closely related to Methanosarcinales, Methanomicrobiales and distinct phylogenetic lineages within the ANME groups. The most frequently recovered phylotypes in the ANME library were related to either ANME-2 or Methanomicrobiales. In contrast to the two archaeal libraries, bacterial diversity was higher with the majority of the 126 bacterial clones most closely related to uncultured clones dominated by the delta- and epsilon-Proteobacteria. Interestingly, while 82% of the clones in the SRB Group 5 library were affiliated with delta-Proteobacteria, the vast majority (83%) of clones in the SRB Group 6 library was affiliated with the Firmicutes. This is the first phylogenetic-based description of microbial communities extant in methane-rich hydrate-associated sediments from a hydrocarbon seep region in the Gulf of Mexico.

show abstract

Section: Phylogenetic Diversity Of Srbmentioning

confidence: 99%

Microbial diversity in sediments associated with surface-breaching gas hydrate mounds in the Gulf of Mexico

Mills

Hodges

Wilson

et al. 2003

FEMS Microbiology Ecology

125

111

View full text Add to dashboard Cite

show abstract

“…wide spectrum of metabolites, such as volatile fatty acids (VFAs), alcohols, phenols, and other substances, as well as large amounts of gas (CO 2 and H 2 ), are produced (11,(38)(39)(40)(41). Some of them, such as butyric and caproic acids, can be used as precursors to and directly as important flavor compounds of CSAL (7,12); caproic acid-producing C. kluyveri strains in particular involve the formation of ethyl caproate, which is the key flavor compound affecting CSAL quality (12).…”

Section: Figmentioning

confidence: 99%

Illuminating Anaerobic Microbial Community and Cooccurrence Patterns across a Quality Gradient in Chinese Liquor Fermentation Pit Muds

Ren

et al. 2016

Appl Environ Microbiol

146

139

View full text Add to dashboard Cite

Fermentation pit mud, an important reservoir of diverse anaerobic microorganisms, is essential for Chinese strong-aroma liquor production. Pit mud quality, according to its sensory characteristics, can be divided into three grades: degraded, normal, and high quality. However, the relationship between pit mud microbial community and pit mud quality is poorly understood, as are microbial associations within the pit mud ecosystem. Here, microbial communities at these grades were compared using Illumina MiSeq sequencing of the variable region V4 of the 16S rRNA gene. Our results revealed that the pit mud microbial community was correlated with its quality and environmental factors. Species richness, biodiversity, and relative and/or absolute abundances of Clostridia, Clostridium kluyveri, Bacteroidia, and Methanobacteria significantly increased, with corresponding increases in levels of pH, NH 4 ؉ , and available phosphorus, from degraded to high-quality pit muds, while levels of Lactobacillus, dissolved organic carbon, and lactate significantly decreased, with normal samples in between. Furthermore, 271 pairs of significant and robust correlations (cooccurrence and negative) were identified from 76 genera using network analysis. Thirteen hubs of cooccurrence patterns, mainly under the Clostridia, Bacteroidia, Methanobacteria, and Methanomicrobia, may play important roles in pit mud ecosystem stability, which may be destroyed with rapidly increased levels of lactic acid bacteria (Lactobacillus, Pediococcus, and Streptococcus). This study may help clarify the relationships among microbial community, environmental conditions, and pit mud quality, allow the improvement of pit mud quality by using bioaugmentation and controlling environmental factors, and shed more light on the ecological rules guiding community assembly in pit mud. Chinese strong-aroma liquor (CSAL), a traditional alcoholic beverage, accounts for Ͼ70% of both national liquor industry production (12 billion liters) and sales volume ($80 billion) (1, 2). Its production is a typical recycling process using solid-state fermentation. In brief, fermented grains obtained from the last fermentation round are mixed with crushed raw materials (sorghum, wheat, corn, rice, and sticky rice) for distillation to give CSAL (3). After cooling, the steamed mixture is supplied with a 10 to 20% (wt/wt) Daqu starter. Next, the above-mentioned mixture is placed into a fermentation vessel (underground cuboid soil pit, 2 m wide by 3 m long by 2 m deep), sealed, and anaerobically fermented for about 60 days at 28 to 32°C (2). The inside walls of the pit are covered with fermentation pit mud (FPM), which is prepared initially by incubating the mixture of clay, spent grain, bean cake powder, and fermentation bacteria (e.g., Clostridium spp.) (2). After fermentation, the fermented grains taken out of the pit are distilled to give liquor after new raw materials are supplied, and then the mixture is applied to the next round of fermentation, as described above. It is widely believe...

show abstract

“…The intensity of the peaks is indicated with the different gray scale, and the y-axis indicates the relation between the peak color and its intensity of the latter are non-saccharolytic and rely on peptone for growth (Ezaki et al, 2001;Ueki et al, 2009). Recently, many protein anaerobic degraders were reported such as Clostridium thiosulfatireducens (Hernández-Eugenio et al, 2002), Clostridium tunisiense (Thabet et al, 2004), and Proteiniphilum acetatigenes (Chen & Dong, 2005). These bacteria were characterized by the use of proteinaceous compounds.…”

Section: Strain Marseille-p4678 T Identificationmentioning

confidence: 99%

Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description

et al. 2018

View full text Add to dashboard Cite

With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un‐isolated bacterial species and describe it via the taxono‐genomics approach. While performing a descriptive study of the human gut microbiota of the pygmy people, strain Marseille‐P4678 T has been isolated from a stool sample of a healthy 39‐year‐old pygmy male. Cells of this strain were Gram‐positive cocci, spore‐forming, non‐motile, catalase‐positive and oxidase‐negative, and grow optimally at 37°C under anaerobic conditions. Its 16S rRNA gene sequence exhibited 89.69% of sequence similarity with Parvimonas micra strain 3119 B T ( NR 036934.1), its phylogenetically closest species with standing in nomenclature. The genome of strain Marseille‐P4678 T is 2,083,161 long with 28.26 mol% of G+C content. Based on its phenotypic, biochemical, genotypic and proteomic profile, this bacterium was classified as a new bacterial genus and species Miniphocibacter massiliensis gen. nov., sp. nov. with the type strain Marseille‐P4678 T .

show abstract

Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor.

Cited by 27 publications

References 46 publications

Microbial diversity in sediments associated with surface-breaching gas hydrate mounds in the Gulf of Mexico

Microbial diversity in sediments associated with surface-breaching gas hydrate mounds in the Gulf of Mexico

Illuminating Anaerobic Microbial Community and Cooccurrence Patterns across a Quality Gradient in Chinese Liquor Fermentation Pit Muds

Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description

Contact Info

Product

Resources

About