Clostridium prolinivorans sp. nov., a thermophilic bacterium isolated from an anaerobic reactor degrading propionate

Abstract

An anaerobic, Gram-stain-positive, spore-forming bacterium, designated strain PYR-10T, was isolated from a mesophilic methanogenic consortium. Cells were 0.7–1.2×6.0–6.3 µm, straight or slightly curved rods, with flagellar motility. Growth was observed in PYG (peptone-yeast glucose) medium at pH 5.5–8.0 (optimum, pH 6.5), 30–55 °C (45 °C) and in NaCl concentrations of 0–15 g l−1 (0 g l−1). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PYR-10T belongs to the genus Clostridium . The strain showed 95.4, 93.7, 93.5 and 93.0 % 16S rRNA gene sequence similarity to Clostridium swellfunianum DSM 27788T, Clostridium pascui DSM 10365T, Clostridium pasteurianum DSM 525T and Clostridium punense DSM 28650T, respectively. The genomic DNA G+C content was 27.7 mol%. The major cellular fatty acids of strain PYR-10T were iso-C15 : 0, C16 : 0, C16 : 0 DMA, anteiso-C15 : 0 and C14 : 0. The main polar lipids were glycolipid, phosphoaminoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylethanolamine and lipids. An unknown menaquinone was detected. 2,6-Diaminopimelic acid was not detected. The whole-cell sugars contained ribose and lower amounts of glucose. Based on the results of phylogenetic, chemotaxonomic and phenotypic analyses, strain PYR-10T represents a novel species of the genus Clostridium , for which the name Clostridium prolinivorans sp. nov. is proposed. The type strain is strain PYR-10T (=JCM 33161T=CCAM 531T=CGMCC 1.5286T).

Aminipila luticellarii sp. nov., an anaerobic bacterium isolated from the pit mud of strong aromatic Chinese liquor, and emended description of the genus Aminipila

“…The pH values were adjusted with sterile anaerobic 1 M HCl, 1 M NaOH, and the buffer solutions (20 mM) described by Ma et al . [25]. A LAQUAtwin pH meter (B-712, Horiba) was used to measure all pH values.…”

“…The physiological and biochemical characteristics of strain JN-18 T and the reference strain DSM 103574 T were determined simultaneously. Growth was determined at temperatures of 10,15,20,25,30,35,37,40,45 and 50 °C in RCM at pH 6.5; and at pH 4-10 (in intervals of 0.5 pH units) at 37 °C. The pH values were adjusted with sterile anaerobic 1 M HCl, 1 M NaOH, and the buffer solutions (20 mM) described by Ma et al [25].…”

“…Growth was determined at temperatures of 10,15,20,25,30,35,37,40,45 and 50 °C in RCM at pH 6.5; and at pH 4-10 (in intervals of 0.5 pH units) at 37 °C. The pH values were adjusted with sterile anaerobic 1 M HCl, 1 M NaOH, and the buffer solutions (20 mM) described by Ma et al [25]. A LAQUAtwin pH meter (B-712, Horiba) was used to measure all pH values.…”

Clostridium weizhouense sp. nov., an anaerobic bacterium isolated from activated sludge of petroleum wastewater

“…Optimum culture condition tests were performed in the RCM, including temperature, pH, and salinity (NaCl w/v). Strain YB-6 T was culturedat different temperatures (25, 28, 30, 32, 34, 36, 37, 38, 40 and 45 °C), pH (4–12, in 1 pH increments),and NaCl concentrations (0, 1, 2, 3, 4,5,6 and 7 %, w/v) for 72 h, and bacterial concentration was evaluated by measuring absorbance of culture mediumat 600 nm using a spectrophotometer [22].…”

“…For the RCM agar plates, 15.0 g l −1 agar was added. Single colonies were picked from RCM agar plates, transferred onto new RCM agar plates, and incubated for 7 days under nitrogen gas [21,22]. The air in the RCM was exhausted using nitrogen gas.…”