Cloning, sequencing, and characterization of the murine nm23-M5 gene during mouse spermatogenesis and spermiogenesis

Hwang, Kyu‐Chan; Ok, Do-Won; Hong, Jong-Chan; Kim, Myeong-Ok; Kim, Jin‐Hoi

doi:10.1016/s0006-291x(03)00916-1

Cited by 25 publications

(34 citation statements)

References 30 publications

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“…NME5 was one of the top heat-regulated genes in the imported strain, with an induction of 5.7-fold compared to 8°C. In mice, the encoded nucleoside diphosphate kinase conferred protection from cell death via the BCL family member Bax, regulating the cellular level of several antioxidants following oxidative stress (Hwang et al 2003). Nevertheless, we could not detect a significantly enhanced accumulation of further antioxidants in response to mild heat or cold in imported trout.…”

Section: Cell Death and Stress Responsecontrasting

confidence: 66%

Impact of Thermal Stress on Kidney-Specific Gene Expression in Farmed Regional and Imported Rainbow Trout

et al. 2015

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Seasonal water temperatures can be stressful for fish in aquaculture and can therefore negatively influence their welfare. Although the kidney is the crucial organ associated with the primary stress response, knowledge about the stress-modulated kidney transcriptome in salmonids is limited. In the present study, we used a comparative microarray approach to characterize the general gene expression profiles of rainbow trout trunk kidney after a 2-week acclimation to mild heat (23 °C) and cold stress (8 °C). Hypothesizing that local adaptation influences stress performance, we aimed to identify differences in the temperature-induced gene expression in the regional trout strain BORN, in addition to a common imported strain. Moderate temperature challenge provoked typical stress response clusters, including heat-shock proteins or cold-inducible factors, in addition to altered energy metabolism in trout kidney. Mild cold, in particular, enhanced renal protein degradation processes, as well as mRNA and protein synthesis, while it also triggered fatty acid biosynthesis. Mild heat led to cytoskeleton-stabilizing processes and might have facilitated cell damage and infection. Furthermore, both breeding lines used different strategies for energy provision, cellular defense, and cell death/survival pathways. As a main finding, the genes involved in energy provision showed generally higher transcript levels at both temperatures in BORN trout compared to imported trout, indicating adjusted metabolic rates under local environmental conditions. Altogether, this study provides a general overview of stress-induced transcriptional patterns in rainbow trout trunk kidney, in addition to identifying genes and networks that contribute to the robustness of the BORN strain. Our analyses suggest SERPINH1 and CIRBP as general marker genes for heat stress and cold stress in trout, respectively.

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Section: Cell Death and Stress Responsecontrasting

confidence: 66%

Impact of Thermal Stress on Kidney-Specific Gene Expression in Farmed Regional and Imported Rainbow Trout

et al. 2015

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“…In contrast to nme2 genes, other nme genes (i.e., nme3-8) display more specific tissue distributions that are also observed in other vertebrate species. For instance, nme5 and nme8 exhibit a strict testis-specific expression in agreement with existing data in mammals (Munier et al, 1998;Sadek et al, 2001;Hwang et al, 2003;MirandaVizuete et al, 2003). Similarly, nme3 and nme6 are widely distributed in zebrafish tissues and organs in agreement with existing data in mammals (Mehus et al, 1999;Masse Fig.…”

Section: Discussionsupporting

confidence: 91%

The nme gene family in zebrafish oogenesis and early development

Desvignes

Fauvel

Bobe³

2011

Naunyn-Schmiedeberg's Arch Pharmacol

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After the recent report of the expression of several nme genes in the zebrafish gonads, the present study aimed at further analyzing the expression of nme genes in the ovary with special attention for the nme transcripts that are maternally inherited and could thus participate in the determination of oocyte developmental competence. The expression levels of all groups I and II nme genes were characterized by QPCR in a panel of zebrafish tissues. The nme genes exhibiting an ovarian expression were subsequently monitored throughout oogenesis and early development, and their expression sites characterized using in situ hybridization. Here, we show that nme2b1, nme3, nme4, and nme6 are highly expressed in the ovary and present in the zebrafish oocyte throughout oogenesis. While the four transcripts are maternally inherited, nme3 and nme6 display a typical maternal profile and are detected in the zebrafish early embryo. In contrast to nme3, nme6, abundance exhibits a sharp decrease during early embryogenesis. After zygotic genome activation, we observed an increased expression of nme2b1, nme2b2, nme3, and nme6. The present study provides a comprehensive overview of the expression of nme family members during zebrafish oogenesis and early development. In addition, the maternal origin of two nme transcripts in the early embryo is reported here for the first time in any vertebrate species. Together, our observations suggest an important role of the nme family in oocyte and embryo development in vertebrates.

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“…45,68 Again, our findings of hydrocephalus, accumulation of nasal exudates, and abnormalities in spermatogenesis in Nme5 -/-mice strongly suggest that ciliary dysfunction is involved in the pathogenesis of these lesions. Interestingly, the ventricular dilatation/hydrocephalus and nasal exudates in Nme5 -/-mice were more severe than those seen in mice deficient for the related gene Nme7.…”

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confidence: 55%

Congenital Hydrocephalus in Genetically Engineered Mice

et al. 2011

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There is evidence that genetic factors play a role in the complex multifactorial pathogenesis of hydrocephalus. Identification of the genes involved in the development of this neurologic disorder in animal models may elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans. The authors report here a brief summary of findings from 12 lines of genetically engineered mice that presented with autosomal recessive congenital hydrocephalus. This study illustrates the value of knockout mice in identifying genetic factors involved in the development of congenital hydrocephalus. Findings suggest that dysfunctional motile cilia represent the underlying pathogenetic mechanism in 8 of the 12 lines (Ulk4, Nme5, Nme7, Kif27, Stk36, Dpcd, Ak7, and Ak8). The likely underlying cause in the remaining 4 lines (RIKEN 4930444A02, Celsr2, Mboat7, and transgenic FZD3) was not determined, but it is possible that some of these could also have ciliary defects. For example, the cerebellar malformations observed in RIKEN 4930444A02 knockout mice show similarities to a number of developmental disorders, such as Joubert, Meckel-Gruber, and Bardet-Biedl syndromes, which involve mutations in ciliarelated genes. Even though the direct relevance of mouse models to hydrocephalus in humans remains uncertain, the high prevalence of familial patterns of inheritance for congenital hydrocephalus in humans suggests that identification of genes responsible for development of hydrocephalus in mice may lead to the identification of homologous modifier genes and susceptibility alleles in humans. Also, characterization of mouse models can enhance understanding of important cell signaling and developmental pathways involved in the pathogenesis of hydrocephalus.

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Cloning, sequencing, and characterization of the murine nm23-M5 gene during mouse spermatogenesis and spermiogenesis

Cited by 25 publications

References 30 publications

Impact of Thermal Stress on Kidney-Specific Gene Expression in Farmed Regional and Imported Rainbow Trout

Impact of Thermal Stress on Kidney-Specific Gene Expression in Farmed Regional and Imported Rainbow Trout

The nme gene family in zebrafish oogenesis and early development

Congenital Hydrocephalus in Genetically Engineered Mice

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