Cloning of the cDNA from normal brain and brain of patients with Alzheimer's disease in the expression vector lambda GT 11

The use of real-time PCR analysis in a gene expression study of Alzheimer’s disease post-mortem brains

“…Several studies have reported that the integrity of RNA depends mainly on pre-mortem factors (Dodd et al, 1988;Johnston et al, 1997;Octave et al, 1988). Furthermore, Barton et al (1993) discussed in their review that pre-mortem conditions, and post-mortem interval have little effect on RNA, with abundant intact and biologically active RNA in tissues frozen 36 h or more after death (Barton et al, 1993).…”

The use of real-time PCR analysis in a gene expression study of Alzheimer’s disease post-mortem brains

“…Several studies have reported that the integrity of RNA depends mainly on pre-mortem factors (Dodd et al, 1988;Johnston et al, 1997;Octave et al, 1988). Furthermore, Barton et al (1993) discussed in their review that pre-mortem conditions, and post-mortem interval have little effect on RNA, with abundant intact and biologically active RNA in tissues frozen 36 h or more after death (Barton et al, 1993).…”

Biochemical and molecular studies using human autopsy brain tissue

“…Polysomes containing up to six ribosomes per mRNA transcript could be reproducibly obtained, and neither polysome size nor translational efficiency were found to be dependent on age, PMI or agonal state. Finally, RNA isolated from autopsy brain is suitable for use in molecular cloning studies, including the construction of cDNA libraries and full-length DNA clones (Octave et al 1988;Vitek et al 1988;Kobayashi et al 1998;Salim et al 2002;unpublished data). It should be noted, however, that sequences near the 5¢ end have been found to be under-represented in cDNAs made from partially degraded mRNAs, as compared with sequences near the polyA + tail (Swift et al 2002).…”

How Important Are Brain Banks for Alcohol Research?

“…Polysomes from autopsied frontal cortex and cerebellum retain physical and molecular integrity, and neither their size nor translational efficiency depends on age, PMI, or agonal state (Langstrom et al, 1989;Marotta et al, 1981). Finally, RNA isolated from autopsy brain is suitable for use in molecular cloning studies, including the construction of complementary DNA libraries and full-length DNA clones (Octave et al, 1988). It should be noted, however, that sequences near the 5' end are underrepresented in complementary DNAs made from partially degraded mRNAs, compared with sequences near the poly (A)ϩ tail (Swift et al, 2002).…”

The use of real-time PCR analysis in a gene expression study of Alzheimer’s disease post-mortem brains

“…Several studies have reported that the integrity of RNA depends mainly on pre-mortem factors (Dodd et al, 1988;Johnston et al, 1997;Octave et al, 1988). Furthermore, Barton et al (1993) discussed in their review that pre-mortem conditions, and post-mortem interval have little effect on RNA, with abundant intact and biologically active RNA in tissues frozen 36 h or more after death (Barton et al, 1993).…”

Biochemical and molecular studies using human autopsy brain tissue

“…Polysomes containing up to six ribosomes per mRNA transcript could be reproducibly obtained, and neither polysome size nor translational efficiency were found to be dependent on age, PMI or agonal state. Finally, RNA isolated from autopsy brain is suitable for use in molecular cloning studies, including the construction of cDNA libraries and full-length DNA clones (Octave et al 1988;Vitek et al 1988;Kobayashi et al 1998;Salim et al 2002;unpublished data). It should be noted, however, that sequences near the 5¢ end have been found to be under-represented in cDNAs made from partially degraded mRNAs, as compared with sequences near the polyA + tail (Swift et al 2002).…”

How Important Are Brain Banks for Alcohol Research?

“…Polysomes from autopsied frontal cortex and cerebellum retain physical and molecular integrity, and neither their size nor translational efficiency depends on age, PMI, or agonal state (Langstrom et al, 1989;Marotta et al, 1981). Finally, RNA isolated from autopsy brain is suitable for use in molecular cloning studies, including the construction of complementary DNA libraries and full-length DNA clones (Octave et al, 1988). It should be noted, however, that sequences near the 5' end are underrepresented in complementary DNAs made from partially degraded mRNAs, compared with sequences near the poly (A)ϩ tail (Swift et al, 2002).…”

The use of real-time PCR analysis in a gene expression study of Alzheimer’s disease post-mortem brains

“…Several studies have reported that the integrity of RNA depends mainly on pre-mortem factors (Dodd et al, 1988;Johnston et al, 1997;Octave et al, 1988). Furthermore, Barton et al (1993) discussed in their review that pre-mortem conditions, and post-mortem interval have little effect on RNA, with abundant intact and biologically active RNA in tissues frozen 36 h or more after death (Barton et al, 1993).…”

Biochemical and molecular studies using human autopsy brain tissue

“…Polysomes containing up to six ribosomes per mRNA transcript could be reproducibly obtained, and neither polysome size nor translational efficiency were found to be dependent on age, PMI or agonal state. Finally, RNA isolated from autopsy brain is suitable for use in molecular cloning studies, including the construction of cDNA libraries and full-length DNA clones (Octave et al 1988;Vitek et al 1988;Kobayashi et al 1998;Salim et al 2002;unpublished data). It should be noted, however, that sequences near the 5¢ end have been found to be under-represented in cDNAs made from partially degraded mRNAs, as compared with sequences near the polyA + tail (Swift et al 2002).…”

How Important Are Brain Banks for Alcohol Research?

“…Polysomes from autopsied frontal cortex and cerebellum retain physical and molecular integrity, and neither their size nor translational efficiency depends on age, PMI, or agonal state (Langstrom et al, 1989;Marotta et al, 1981). Finally, RNA isolated from autopsy brain is suitable for use in molecular cloning studies, including the construction of complementary DNA libraries and full-length DNA clones (Octave et al, 1988). It should be noted, however, that sequences near the 5' end are underrepresented in complementary DNAs made from partially degraded mRNAs, compared with sequences near the poly (A)ϩ tail (Swift et al, 2002).…”