Cloning of six cherry self-incompatibility alleles and development of allele-specific PCR detection

Sonneveld, T.; Robbins, Timothy P.; Bošković, R.; Tobutt, K. R.

doi:10.1007/s001220000525

Cited by 121 publications

(171 citation statements)

References 23 publications

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“…The putative introns differed considerably in size (Table 1). The nucleotide sequences of the exon regions showed high homology (80-90%) to those of the S-RNases previously reported in P. dulcis (Ushijima et al, 1998) and P. avium (Sonneveld et al, 2001;Wiersma et al, 2001). The alignment of the deduced amino-acid sequences is shown in Figure 3.…”

Section: Genomic Southern Analysissupporting

confidence: 61%

“…This study applied the PCR method using consensus primers as described in the Prunus cultivars (Tao et al, 1999;Sonneveld et al, 2001;Wiersma et al, 2001). This approach was successful in cloning several putative S-RNases and the fragments amplified from the genomic clones of different alleles were distinguished based on the intron length variation.…”

Section: Discussion Determination Of S-genotypementioning

confidence: 99%

“…PCR amplification of S-RNase genes from genomic DNA To amplify S-RNases from genomic DNA, primers C1-sense (5 0 -TATTTTCAATTTGTGCAACA-3 0 ) and C5-antisense (5 0 -CAAAATACCACTTCATGTAA-3 0 ) were designed from conserved domains (C1 and C5) of the P. avium (Sonneveld et al, 2001;Wiersma et al, 2001) and P. dulcis (Ushijima et al, 1998) S-RNases. PCR was performed with 35 cycles at 951C for 30 s, 501C for 30 s, 721C for 1 min with initial denaturing at 951C for 5 min, and a final extension at 721C for 5 min.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The numbers of synonymous (Ks) and nonsynonymous (Ka) substitutions per site were calculated from pairwise comparisons within and between the seven sequences of flowering cherry, and the nine sequences from Prunus cultivars, S 1 , S 2 , S 3 , S 4 , S 5 and S 6 of P. avium (Sonneveld et al, 2001;Wiersma et al, 2001) and S b , S c and S d of P. dulcis (Ushijima et al, 1998). Table 2 shows the results.…”

Section: Sequence Variation Of S-rnasesmentioning

confidence: 99%

“…Vieira and Charlesworth (2002) revealed sequence variation at the S-locus in Scrophulariaceae. In the Rosaceae, molecular techniques for identifying S-alleles by allele-specific PCR are now being developed for crossing and breeding of fruit cultivars (Sonneveld et al, 2001;Wiersma et al, 2001). This paper reports on S-allele diversity in two populations of flowering cherry, Prunus lannesiana var.…”

Section: Introductionmentioning

confidence: 99%

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Allelic diversity of S-RNase at the self-incompatibility locus in natural flowering cherry populations (Prunus lannesiana var. speciosa)

Kato

Mukai

2004

Heredity

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In the Rosaceae family, which includes Prunus, gametophytic self-incompatibility (GSI) is controlled by a single multiallelic locus (S-locus), and the S-locus product expressed in the pistils is a glycoprotein with ribonuclease activity (S-RNase). Two populations of flowering cherry (Prunus lannesiana var. speciosa), located on Hachijo Island in Japan's Izu Islands, were sampled, and S-allele diversity was surveyed based on the sequence polymorphism of S-RNase. A total of seven S-alleles were cloned and sequenced. The S-RNases of flowering cherry showed high homology to those of Prunus cultivars (P. avium and P. dulcis). In the phylogenetic tree, the S-RNases of flowering cherry and other Prunus cultivars formed a distinct group, but they did not form species-specific subgroups. The nucleotide substitution pattern in S-RNases of flowering cherry showed no excess of nonsynonymous substitutions relative to synonymous substitutions. However, the S-RNases of flowering cherry had a higher Ka/Ks ratio than those of other Prunus cultivars, and a subtle heterogeneity in the nucleotide substitution rates was observed among the Prunus species. The S-genotype of each individual was determined by Southern blotting of restriction enzyme-digested genomic DNA, using cDNA for S-RNase as a probe. A total of 22 S-alleles were identified. All individuals examined were heterozygous, as expected under GSI. The allele frequencies were, contrary to the expectation under GSI, significantly unequal. The two populations studied showed a high degree of overlap, with 18 shared alleles. However, the allele frequencies differed considerably between the two populations.

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Section: Genomic Southern Analysissupporting

confidence: 61%

Section: Discussion Determination Of S-genotypementioning

confidence: 99%