Cloning of Hypervariable Minisatellite and Simple Sequence Microsatellite Repeats for DNA Fingerprinting of Important Aquacultural Species of Salmonids and Tilapia

Bentzen, Paul; Harris, Andrew; Wright, Jonathan

doi:10.1007/978-3-0348-7312-3_17

Cited by 62 publications

(37 citation statements)

References 54 publications

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“…For population biology, it was the development of DNA fingerprinting (Jeffreys et al, 1985a,b) that indicated the value of utilizing these highly diverse, tandemly repetitive sequences. Highly polymorphic minisatellite and microsatellite markers (Tautz, 1989) have provided a wealth of genetic variants to examine fish populations (Taggart and Ferguson, 1990a,b;Bentzen et al, 1991;Prodohl, 1992;Wright, 1993;Prodohl et al, 1994), usually enhancing our ability to discriminate individuals and populations. Moreover, amplification of VNTRs using PCR (Saiki et aL, 1988;Bentzen et al, 1991Bentzen et al, , 1993Wright, 1993) removes the technical difficulties of repeated sequence isolation and cloning (Amos, 1992).…”

Section: Nuclear Dna Analysismentioning

confidence: 98%

Molecular genetics and the stock concept in fisheries

Carvalho¹,

Hauser²

1995

Molecular Genetics in Fisheries

218

247

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Section: Nuclear Dna Analysismentioning

confidence: 98%

Molecular genetics and the stock concept in fisheries

Carvalho¹,

Hauser²

1995

Molecular Genetics in Fisheries

218

247

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“…comm. ) and marker-assisted selective breeding in aquaculture (Bentzen et al, 1991;Herbinger et al, 1996;Olsen etal, 1996).…”

Section: Microgeograph Ic Differentiationmentioning

confidence: 99%

Advances in the molecular analysis of fish population structure

Carvalho

Hauser

1998

Italian Journal of Zoology

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The characteristics of high population and species biodiversity, taken together with the major anthropogenic threats arising from exploitation, introductions and habitat alterations, have ensured that fishes have received major attention from molecular biologists in the study of wild populations. Foremost among the various challenges has been the development of techniques to estimate population discreteness and associated patterns of dispersal and gene flow, so-called stock structure analysis. Here, we review some recent developments in molecular technology, especially microsatellite analysis, and consider critically how such markers can be employed to estimate genetic differentiation in species exhibiting high mobility in open waters. Comparisons are made between microsatellites and other genetic markers (protein and DNA) in their ability to detect population structuring. Particular emphasis is placed on the need to design sampling programmes that take account of aspects of the biology of the species under study, and the scale and nature of sample collection. Recent novel applications of PCR-based technology in the analysis of population structure are considered, including the detection of microgeographic differentiation, the analysis of historical fish populations using recovered DNA, and the molecular characterization of allelic variants among populations in relation to function.

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“…In some mammals, certain satellite DNAs may occur as millions of copies of the repeat sequence per cell (Alberts et aL, 1983). Minisatellites contain repeat units from ten to a hundred nucleotides along (Jeffreys et al, 1985b) and are highly polymorphic (Taggart and Ferguson, 1990;Bentzen et al, 1991). Microsatellites contain smaller repeat units, usually one to four nucleotides long, and are variable as well (Tautz, 1989;Bentzen and Wright, 1993); however, research on microsatellites is fairly new and not much information is available on their degree of variation.…”

Section: Non-repetitive and Repetitive Dnasmentioning

confidence: 99%