Cloning of genomic sequences of three crustacean hyperglycemic hormone superfamily genes and elucidation of their roles of regulating insulin-like androgenic gland hormone gene

Li, Fajun; Bai, Hongkun; Zhang, Wenyi; Fu, Hongtuo; Jiang, Fengwei; Liang, Guoxia; Jin, Shubo; Sun, Shengming; Qiao, Hui

doi:10.1016/j.gene.2015.02.012

Cited by 47 publications

(27 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As part of the "eyestalk-AG-testis" endocrine axis, the XO-SG complex was found to have an inhibitory effect on AG development or IAG expression. Eyestalk ablation in decapod crustaceans caused hypertrophy of the AG (Khalaila et al, 2002) and up-regulation of IAG transcripts (Chung et al, 2011;Li et al, 2015). The expression level of LvIAG was significantly up-regulated after unilateral eyestalk ablation.…”

Section: Discussionmentioning

confidence: 94%

“…The X-organ/sinus-gland (XO-SG) complex in the eyestalk is the major neuroendocrine system in crustacean suggested to be an upstream regulator in various endocrine axes. Sexual development related function of neurohormones mainly referred to eyestalk expressed CHH subfamily II members, which were considered as inhibitory factors on ovary development (de Kleijn et al, 1994;Gu et al, 2002;Treerattrakool et al, 2008;Vrinda et al, 2017) and IAG expression (Li et al, 2015). On the other hand, CHH subfamily I members were usually deemed as hyperglycemic factors or osmo-regulators for their functions in regulating crustacean hemolymph glucose levels and ion transport (Chung et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…In Metapenaeus ensis, one type of MIH, MIH-B, showed an inhibitory role in the initiation of vitellogenesis as its expression levels in the eyestalk decreased in the initial phase of ovary maturation (Gu et al, 2002). A recent study revealed that CHH subfamily II members could also regulate male sexual development because silencing of either GIH or MIH could dramatically increase the transcriptional level of IAG in Macrobrachium nipponense (Li et al, 2015). Except for CHH family hormones, the crustacean female sex hormone in Scylla paramamosain was also found to be an inhibitory factor of IAG expression (Liu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei

Guo

et al. 2019

Front. Physiol.

View full text Add to dashboard Cite

The "eyestalk-androgenic gland (AG)-testis" endocrine axis is involved in male sexual differentiation of crustaceans. The insulin-like androgenic gland hormone (IAG), secreted from the AG, plays a central role in this axis, however key factors upstream the IAG are still poorly understood. Here, two crustacean hyperglycemic hormone (CHH) genes (LvCHH1 and LvCHH2) and their putative receptor guanylate cyclase (LvGC) were identified in Litopenaeus vannamei. LvCHH1 and LvCHH2 belonged to CHH subfamily I members and LvGC was a membrane-bound guanylate cyclase. They were all differentially expressed in eyestalks and gonads of males and females. RNA interference (RNAi) of either LvCHH1 or LvCHH2 increased LvIAG expression, while injection of their recombinant protein decreased LvIAG expression, indicating that LvCHH1 and LvCHH2 are inhibitory factors of LvIAG expression. Yeast two-hybrid assay showed that both LvCHH1 and LvCHH2 interacted with LvGC and their RNAi and recombinant protein injection exerted opposite regulatory effects on the transcriptional expression of LvGC. Meanwhile, knockdown of LvGC increased LvIAG expression. These results suggest that LvGC is the receptor of LvCHH1 and LvCHH2 and they are all involved in male sexual development by regulating LvIAG expression. The present study unveils missing upstream elements in the "eyestalk-AG-testis" endocrine axis in crustacean.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei

Guo

et al. 2019

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…The function of insulin-like androgenic gland (IAG) is believed to be similar to that of the isopod AG hormone, considered as a key regulator of male sex-determination and development (Tomer and Sagi, 2012). In M. nipponense, IAG was reported to be involved in sex-determination (Ma et al, 2013), and has been shown to regulate the expression of three crustacean genes belonging to the hyperglycemic hormone superfamily (Li et al, 2015a) as well as the gene coding for insulin-like androgenic gland hormonebinding protein (Li et al, 2015b). Four genes, sex-lethal (Sxl), transformer (Tra), transformer-2 (Tra-2), and double-sex (Dsx), regulate the sex-determination pathway in Drosophila melanogaster.…”

Section: Discussionmentioning

confidence: 99%

Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense

Jin¹,

Fu²,

Jiang³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China. The androgenic gland produces hormones that play crucial roles in the differentiation of crustaceans to the male sex. MicroRNA (miRNA) post-transcriptionally regulates many protein-coding genes, influencing important biological and metabolic processes. However, currently, there is no published data identifying miRNA in M. nipponense. In this study, we identified novel miRNA in the androgenic gland of M. nipponense. Using the high-throughput Illumina Solexa system, 1077 miRNA were identified from small RNA libraries by aligning with the de novo androgenic gland transcriptome of M. nipponense (obtained from RNA-Seq) and the sequences in the miRBase21 database. A total of 8,248, 76,011, and 78,307 target genes were predicted in the EST and SRA sequences provided in the NCBI database, and the androgenic gland transcriptome of M. nipponense, respectively. Some potential sex-18397 Identification of androgenic gland microRNA ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18396-18406 (2015) related miRNA were identified based on the function of the predicted target genes. The results of our study provide new information regarding the miRNA expression in M. nipponense, which could be the basis for further genetic studies on decapod crustaceans.

show abstract

“…Recently, the influence of these neurohormones has been explicitly demonstrated. Li et al [2015] Hence to summarize, it appears that the mature AG has minimal transcriptional distinction when compared with non-AG tissues, other than its production of Sv-IAG. However, these expression data provide transcriptomic support for the effects of eyestalk-ablation, demonstrating the upstream influences that neuroendocrine regulation has on AG functioning.…”

Section: Functional Characterization Of the Mature Androgenic Gland Dmentioning

confidence: 94%

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

Chandler

Aizen²,

Elizur³

et al. 2015

Sex Dev

View full text Add to dashboard Cite

The Eastern spiny lobster, Sagmariasus verreauxi, is commercially important in fisheries, with growing aquaculture potential, driving an interest to better understand male sexual differentiation. Amongst the Decapoda, the androgenic gland (AG) and the insulin-like androgenic gland hormone (IAG) have a well-defined function in male sexual differentiation. However, IAG is not a sex determinant and therefore must be considered as part of a broader, integrated pathway. This work uses a transcriptomic, multi-tissue approach to provide an integrated description of male-biased expression as mediated through the AG. Transcriptomic analyses demonstrate that IAG expression is stage- and eyestalk-regulated (low in immature, high in mature and 6-times higher in hypertrophied glands), with IAG being the predominant AG-specific factor. The low expression of this key factor in immature males suggests the involvement of other tissues in male sexual differentiation. Across tissues, the gonad (87.8%) and antennal gland (73.5%) show the highest male-biased differential expression of transcripts and also express 4 sex-determination regulators, known as Dmrts, with broader expression of Sv-Sxl and Sv-TRA-2. In order to better understand male sexual differentiation, tissues other than the AG must also be considered. This research highlights the gonad and antennal gland as showing significant male-biased expression patterns, including the Sv-Dmrts.

show abstract

Cloning of genomic sequences of three crustacean hyperglycemic hormone superfamily genes and elucidation of their roles of regulating insulin-like androgenic gland hormone gene

Cited by 47 publications

References 60 publications

Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei

Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei

Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

Contact Info

Product

Resources

About