Cloning of a functional gene responsible for the expression of a cell surface antigen correlated with human chronic lymphocytic leukemia

Stanners, Clifford P.; Lam, Teresa; Chamberlain, John W.; Stewart, S. S.; Price, Gerald B.

doi:10.1016/0092-8674(81)90375-5

Cited by 30 publications

(29 citation statements)

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“…HCH-1 is the human CLL-Chinese hamster cell line previously described by Stanners et al (2). LR-73 is the ts revertant of L-73, a CHO line with flat morphology and more normal growth control characteristics derived from ts Hi (5).…”

Section: Materials and Methods Cell Linesmentioning

confidence: 99%

“…library of the hybrid cell, HCH-1, by hybridization with labelled total human. genomic DNA, coupled with a demonstration of relatively weak antigen-inducing activity (2). Its basic structure is shown in Figure 5.…”

Section: Genomic Organization Of Hsag-1-like Sequencesmentioning

confidence: 99%

“…HSAG-1 and HSAG-2 were isolated from a library of EcoRI fragments of HCH-1 genomic DNA cloned in Agt WES (2). pHSAG-1 is the 3.4 kb insert of HSAG-1 and pHSAG-2 the 9.5 kb insert of HSAG-2 cloned into the EcoRI site of pBR322.…”

Section: Hsag Clones and Subclonesmentioning

confidence: 99%

“…The copy number of functional HSAG elements was first estimated during the cloning of HSAG-1 and HSAG-2 to be 100 to 1000 per haploid genome (2), while preliminary estimates of the copy number of elements with nucleotide sequence homology to the antigen-eliciting portion of HSAG-1 were in the thousands in both CLL cell and CHO cell genomes (4). As 80% of a number of these HSAG-1 homologous clones isolated from a CLL genomic library were shown to be functional, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…This antibody was shown to react with the surface of a very limited range of human leukemia cells including cells from peripheral blood and bone marrow of CLL patients, but not with any of the normal cells tested (1). HSAG-1, the prototype member, was cloned from HCH-1, a CLL-CHO hybrid cell, by the isolation of clones containing human-specific reiterated sequences from a portion of a genomic library of HCH-1 capable of producing antigen after transfection of L-strain mouse cells (2). The structure of Nucleic Acids Research both in its right hand end, which contained Alu sequences quite closely related to CHO and human Alu consensus sequences, and in its 1.4 kb antigen-eliciting left hand end which contained highly diverged Alu-like sequences (3).…”

Section: Introductionmentioning

confidence: 99%

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Studies on HSAG, a middle repetitive family of genetic elements which elicit a leukemia-related cellular surface antigen

Beitel¹,

Chamberlain²,

Benchimol³

et al. 1986

Nucl Acids Res

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HSAG is a family of genetic elements capable of eliciting, in transfected cells, a cellular surface antigen which is correlated with human chronic lymphocytic leukemia (CLL). Its prototype member, HSAG-1, was cloned as a 3.4 kb insert and contains numerous Alu-related elements, including its left hand 1.4 kb antigen-eliciting end. These elements are present in mammalian cells with copy numbers varying from 7,000 to 200,000 per haploid genome, depending on how closely their sequence conforms to the Alu consensus sequence. They are present in the configuration found in HSAG-1, a 3.4 kb EcoRI fragment which is part of a larger unit of at least 12.7 kb, at a frequency of 20-50 per haploid genome, and dispersed around the genome. A second family member, HSAG-2, isolated using a functional assay, was cloned as a 9.5 kb insert and contained a 1.5 kb antigen-eliciting left hand end. As in HSAG-1, the antigen-eliciting portion of the insert also contained Alu-like elements, unlike most of the remainder of the insert.A number of HSAG family members were cloned from a library of human CLL genomic DNA by sequence homology with the antigen-eliciting portion of HSAG-1. Most of these members were also shown to be capable of eliciting antigen. Their only sequence similarity with HSAG-1 appeared to be in their content of numerous Alu-like elements. The evidence thus supports the view that the HSAG functional family consists of clusters of Alu-like elements.

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Section: Materials and Methods Cell Linesmentioning

confidence: 99%

Section: Genomic Organization Of Hsag-1-like Sequencesmentioning

confidence: 99%

Section: Hsag Clones and Subclonesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%