Cloning, expression and purification of cytochromec₆from the brown algaHizikia fusiformisand complete X-ray diffraction analysis of the structure

Abstract

The primary sequence of cytochrome c(6) from the brown alga Hizikia fusiformis has been determined by cDNA cloning and the crystal structure has been solved at 1.6 A resolution. The crystal belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 84.58, c = 232.91 A and six molecules per asymmetric unit. The genome code, amino-acid sequence and crystal structure of H. fusiformis cytochrome c(6) were most similar to those of red algal cytochrome c(6). These results support the hypothesis that brown algae acquired their chloroplasts via secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host.

Mannitol-1-phosphate dehydrogenase activity in Ectocarpus siliculosus, a key role for mannitol synthesis in brown algae

“…So far, There were only a few examples of successful expression of brown algal proteins in heterologous system: a cytochrome c 6 from Hizikia fusiformis (Akazaki et al 2008), and GSTs (Glutathione S-transferases) from Laminaria digitata (Hervé et al 2008) and E. siliculosus (de Franco et al 2009). EsM1PDH1 is the first enzyme of the primary metabolism of E. siliculosus shown to be active in E. coli.…”

Atomic‐resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion

“…The structures of cytochromes c 6 from three cyanobacteria ( Arthrospira maxima [10], Synechococcus elongatus [11], and P. laminosum [7]), one from a red alga ( Porphyra yezoensis [12]), one from a brown alga ( Hizikia fusiformis [13]), and four from green algae ( Monoraphidium brauni [14], C. reinhardtii [15], Scenedesmus obliquus [16], and Cladophora glomerata [17]) have been determined, as has the structure of cytochrome c 6A from A. thaliana [18]. Together, these data represent a large volume of information about the sequences and structures of cytochrome c 6 .…”

Cytochrome c 6 of Cyanobacteria and Algae: From the Structure to the Interaction

“…330 mV at pH 7. Frazao et al 1995), Scenedesmus obliquus (1c6o; Schnackenberg et al 1999), Cladophora glomerata (1ls9; Dikiy et al 2002) and Chlamydomonas reinhardtii (1cyi; Kerfeld et al 1995), the red alga Porphyra yezoensis (1gdv; Yamada et al 2000) , the dun alga Hizikia fusiformis (2zbo; Akazaki et al 2008), and the cyanobacteria Synechococcus sp. (4eic; Bialek et al 2009), Synechococcus elongatus (1c6s; Beissinger et al 1998), Phormidium laminosum (2v08; Worrall et al 2007) and Arthrospira maxima (1kib; Kerfeld et al 2002).…”