2013
DOI: 10.1016/j.jbiosc.2012.08.016
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Cloning, expression and biochemical characterization of a novel, moderately thermostable GDSL family esterase from Geobacillus thermodenitrificans T2

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Cited by 33 publications
(27 citation statements)
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“…The optimal pH of RmEstB is 7.5, which is similar to that of most other reported esterases [18,35,43], higher than that of a HSL esterase from Pyrobaculum calidifontis [4] and RmEstA from R. miehei [29], and lower than that of a HSL esterase from a soil DNA library [42] and EstK from P. mandelii [39]. Most microbial esterases exhibit narrow pH stabilities under alkaline conditions [11,38,44]. However, RmEstB in the present study also showed excellent stability across a broad range of pHs (4.5-10.1) (Fig.…”
Section: Discussionsupporting
confidence: 67%
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“…The optimal pH of RmEstB is 7.5, which is similar to that of most other reported esterases [18,35,43], higher than that of a HSL esterase from Pyrobaculum calidifontis [4] and RmEstA from R. miehei [29], and lower than that of a HSL esterase from a soil DNA library [42] and EstK from P. mandelii [39]. Most microbial esterases exhibit narrow pH stabilities under alkaline conditions [11,38,44]. However, RmEstB in the present study also showed excellent stability across a broad range of pHs (4.5-10.1) (Fig.…”
Section: Discussionsupporting
confidence: 67%
“…The substrate specificity of RmEstB also differed from that of RmEstA, which showed highest activities for pNPH and tributyrin (C 4 ) [29]. It is interesting that RmEstB also displayed activities toward pNP esters with carbon chains longer than 10 (C 10 -C 14 ) (Table 3), a property which is distinct from most other reported esterases [6,15,44].…”
Section: Discussionmentioning
confidence: 89%
“…To date, all reported microbial HSL esterases have been from bacteria, such as Archaeoglobus fulgidus [37], Rheinheimera sp. [38] and Geobacillus thermodenitrificans T2 [16]. RmEstA is the first reported fungal HSL esterase.…”
Section: Discussionmentioning
confidence: 99%
“…Many bacterial esterase genes have been cloned and expressed, such as from Pseudoalteromonas sp. 643A [4], Oenococcus oeni [12], P. arctica [13], Thermoanaerobacter tengcongensis [14], Pseudomonas putida [15] and Geobacillus thermodenitrificans T2 [16]. Several esterase genes have also been isolated and cloned from metagenomic libraries such as fermented compost [17], soil [18], and intertidal flat sediment [19].…”
Section: Introductionmentioning
confidence: 99%
“…Besides, the purified esterase also showed better activity against C6 (pNPH), C8 (pNPC), C10 (pNPD) and C12 (pNPL). The reported esterases from deep-sea metagenomic library [38], Oenococcus oeni [39], Geobacillus thermodenitrificans T2 [40], Thermus thermophilus HB27 [13] had also shown a strong preference for the hydrolysis of pNPB. However the present esterase with broad substrate specificity offers potential applications in the production of various high value products.…”
Section: Chemicalsmentioning
confidence: 99%