“…With this framework, we found, after induction by copper addition, that U. piniceus, but not Rhodotorula spp., responded as reported (Gralla et al, 1991;Ramírez-Orozco et al, 1999). To discard an inactive form of Cu,Zn-SOD in the Rhodotorula spp., we made a Western blot analysis, finding no recognition by polyclonal antibodies raised against D. hansenii Cu,Zn-SOD enzyme (Hernández-Saavedra and Ochoa, 1999), contrary to different recognition levels observed for other species (data not shown; Hernández-Saavedra and Romero, 2001). From these results, we assumed absence of the Cu,Zn-SOD enzyme in the studied Rhodotorula spp.…”