Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish

Harms, Craig A.; Kennedy‐Stoskopf, Suzanne; Horne, William A.; Fuller, Frederick J.; Tompkins, W. A. F.

doi:10.1006/fsim.1999.0230

Cited by 57 publications

(33 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the rainbow trout, constitutive expression of TGF 1 was found in the brain, gills, spleen, head kidney and peripheral blood leucocytes but not in the liver [22]. In the hybrid striped bass, although di#erences in the level of expression were seen using an RT-quantitative PCR (RT-qcPCR), constitutive expression was found in spleen, head kidney and peripheral blood leucocytes [23]. Constitutive expression of TGF 1 was also found in the macrophage-like cell line RTS 11 derived from rainbow trout spleen [38].…”

Section: Discussionmentioning

confidence: 95%

“…Within this mature peptide region, nine conserved cysteines were found, eight of which form intrachain disulphide bonds, while the remaining cysteine forms an interchain disulphide bond [33,34]. In the case of sea bream TGF 1, the first cysteine is in position 8 as is the case with other sequenced teleost TGF 1 sequences [22][23][24], in contrast to mammalian TGF 1 genes where it is at position 7.…”

Section: Discussionmentioning

confidence: 98%

“…In fish, it has been demonstrated that three di#erent TGF isoforms exist. The cDNA sequence for TGFs equivalent to TGF 1 were described in the rainbow trout [22], hybrid striped bass (Morone saxatilis M. chrysops) [23] and carp, C. carpio [24], while an equivalent to TGF 2 was isolated in the carp [25]. Moreover, a partial sequence of an equivalent of TGF 3 was isolated from Siberian sturgeon (Acipenser baeri), rainbow trout (O. mykiss) and European eel (Anguilla anguilla) [26].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular characterisation of sea bream (Sparus aurata) transforming growth factor β1

Tafalla

Aranguren

Secombes

et al. 2003

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterisation of sea bream (Sparus aurata) transforming growth factor β1

Tafalla

Aranguren

Secombes

et al. 2003

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Similarly, the filial generations of O. mossambicus (♀) and O. niloticus (♂) have a faster growth rate than the parents [61]. Last, the hybrid offspring of Morone sexatilis (♀) and Morone chrysops (♂) males exhibit obvious heterosis [62]. In recent years, the domestic scientists processed much interspecific hybridization of fish in Cyprinidae, Paralichthyidae [63], Siluridae, Sparidae, Cichlid, Percichthyidae, etc.…”

Section: Cross Breedingmentioning

confidence: 99%

Development and application of biological technologies in fish genetic breeding

Duan

Xiao

et al. 2015

Sci. China Life Sci.

View full text Add to dashboard Cite

Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties. For the purpose of genetic improvement, researchers can select for desirable genetic traits, integrate a suite of traits from different donors, or alter the innate genetic traits of a species. These improved varieties have, in many cases, facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield. In this review, we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies (containing traditional selective breeding, molecular marker-assisted breeding, genome-wide selective breeding and breeding by controlling single-sex groups), integration breeding technologies (containing cross breeding, nuclear transplantation, germline stem cells and germ cells transplantation, artificial gynogenesis, artificial androgenesis and polyploid breeding) and modification breeding technologies (represented by transgenic breeding) in fish genetic breeding. Additionally, we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish, including distant hybridization, gynogenesis, and androgenesis. Finally, we systematically summarize the research status and known problems associated with each technology. fish genetic breeding, genetic improvement, biological method, traits, new variety Citation:

show abstract

“…The band intensities, obtained by densitometric scanning, were analyzed using the Multi-Analyst software (Bio-Rad). Difference in intensity due to size difference of the two sbGHR amplicons has also been taken into account in the calculation of the relative expression values (Harms et al 2000). The target band intensity was divided by the -actin band intensity in each tissue for normalization.…”

Section: Expression Of Sbghr1 and Sbghr2 In Seabream Tissuesmentioning

confidence: 99%

The co-existence of two growth hormone receptors in teleost fish and their differential signal transduction, tissue distribution and hormonal regulation of expression in seabream

Jiao

Huang²,

Chan³

et al. 2006

Journal of Molecular Endocrinology

148

View full text Add to dashboard Cite

Two genomic contigs of putative growth hormone receptors (GHRs) were identified in fugu and zebrafish genomes by in silico analysis, suggesting the presence of two GHR subtypes in a single teleost species. We have tested this hypothesis by cloning the full-length cDNA sequence of a second GHR subtype from the black seabream in which the first GHR subtype had been previously reported by us. In addition, we had also cloned the sequences of both GHR subtypes from two other fish species, namely the Southern catfish and the Nile tilapia. Phylogenetic analysis of known GHR sequences from various vertebrates revealed that fish GHRs cluster into two distinct clades, viz. GHR1 and GHR2. One clade (GHR1), containing 6 to 7 extracellular cysteine residues, is structurally more akin to the non-teleost GHRs. The other clade (GHR2), containing only 4 to 5 extracellular cysteine residues, is unique to teleosts and is structurally more divergent from the non-teleost GHRs. In addition, we had examined the biological activities of both GHR subtypes from seabream using a number of reporter transcription assays in cultured eukaryotic cells and demonstrated that both of them were able to activate the Spi 2·1 and -casein promoters upon receptor stimulation in a ligand specific manner. In contrast, only GHR1 but not GHR2 in seabream could trigger the c-fos promoter activity, indicating that the two GHR subtypes possess some differences in their signal transduction mechanisms. Also, the expression of GHR2 is significantly higher than GHR1 in many tissues of the seabream including the gonad, kidney, muscle, pituitary and spleen. In vivo hormone treatment data indicated that cortisol upregulated hepatic GHR1 expression in seabream but not GHR2, whereas testosterone decreased hepatic GHR2 expression but not GHR1. On the other hand, hepatic expression of both GHR1 and GHR2 in seabream was decreased by estradiol treatment.

show abstract

Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish

Cited by 57 publications

References 27 publications

Molecular characterisation of sea bream (Sparus aurata) transforming growth factor β1

Molecular characterisation of sea bream (Sparus aurata) transforming growth factor β1

Development and application of biological technologies in fish genetic breeding

The co-existence of two growth hormone receptors in teleost fish and their differential signal transduction, tissue distribution and hormonal regulation of expression in seabream

Contact Info

Product

Resources

About