Cloning and sequence analysis of the 18 S ribosomal RNA gene of tomato and a secondary structure model for the 18 S rRNA of angiosperms

Schmidt-Puchta, Waltraud; Kütemeier, Gertrud; Günther, Isolde; Haas, Bernd; Sänger, Heinz L.

doi:10.1007/bf00261152

Cited by 17 publications

(16 citation statements)

References 40 publications

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“…The sequence alignment, also, allowed to corroborate a previous report by Shimdt-Puchta et al [12] who proposed four variable regions in the 18S rDNA. These variable regions are underlined in Fig.…”

supporting

confidence: 76%

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Sequence of 18S rDNA of actinorhizal Alnus glutinosa (Betulaceae)

Savard

Lalonde

1991

Plant Mol Biol

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The small subunit ribosomal DNA for a woody actinorhizal, Alnus glutinosa, was isolated by the PCR method. Amplification products were cloned into the Bluescript SK- vector. Full sequence, 1698 bp, was obtained with NS1 to NS8 primers. Sequence alignments were made by UWGCG sequence data analysis computer programs. 18S rDNA sequence of A. glutinosa was compared to analogous segments of four other angiosperms, tomato, rice, maize and soybean. Sequence homologies are discussed and application for the technique is suggested.

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supporting

confidence: 76%

“…Each transcription unit consists of a 18S, 5.8S and 28S rRNA gene separated by non-coding intergenic spacers (IGS) [12]. rRNA coding sequences have been highly conserved during evolution, indicating a strong positive selection pressure [11].…”

mentioning

confidence: 99%

Sequence of 18S rDNA of actinorhizal Alnus glutinosa (Betulaceae)

Savard

Lalonde

1991

Plant Mol Biol

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“…The gene-specific PCR fragments (approximately 330 bp) of DcSUT1 and DcSUT2 were labeled with 32 P by random priming (Sambrook et al, 1989). As a probe for a constitutively expressed gene, a 1700-bp-long fragment (EcoRI/EcoRI) of the 18S rRNA gene from tomato was used (Schmidt-Puchta et al, 1989). The blots were prehybridized at 65°C for 5 h in 6ϫ SSC, 5ϫ Denhardt's solution, 100 mg/mL denatured calf thymus DNA, and 0.5% SDS (Sambrook et al, 1989).…”

Section: Analysis Of Rna and Dnamentioning

confidence: 99%

Characterization of Source- and Sink-Specific Sucrose/H+ Symporters from Carrot

Shakya

Sturm

1998

Plant Physiology

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To understand how sucrose (Suc) is transported from source leaves to developing tap roots of carrot (Daucus carota L.), we cloned two cDNAs (DcSUT1 and DcSUT2) for proteins with homologies to plant Suc/H ؉ symporters. The deduced polypeptide sequences are 52% identical and have 12 predicted membranespanning domains each. Transport activities were confirmed by expression of the clones in yeast cells. Both transporters had optimal activity below pH 5.0 and Michaelis constant values of 0.5 mM. Suc uptake was inhibited by protonophores, suggesting that Suc transport is linked to the proton electrochemical potential across the plasma membrane. DcSUT1 and DcSUT2 had markedly different expression patterns. Transcripts of DcSUT1 were found only in the green parts of plants, with highest levels in the lamina of source leaves, indicating that DcSUT1 is required for the loading of Suc into the phloem. In leaf lamina expression was diurnally regulated, suggesting that Suc export from the leaves is higher during the day than during the night. The mRNA of DcSUT2 was found mainly in sink organs, and no diurnal expression pattern was detected in the storage root. Here, expression was not restricted to the phloem but was much higher in storage parenchyma tissues of phloem and xylem. The close relationship of DcSUT2 with a Suc/H ؉ symporter from fava bean, which facilitates Suc uptake into the cotyledons of developing seeds, indicates that this carrot Suc transporter may be involved in loading Suc into storage parenchyma cells.

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“…The intensity of labeling was measured with a PhosphoImager (Molecular Dynamics, Sunnyvale, CA) arid is expressed in arbitrary units. The hybridization probes iised were: the 1-kb PstI fragment of tobacco class I P-l,3-.g'lucanase cDNA clone pGL43 (Shinshi et al, 1988), the 1.2-kb XhoIlXbaI fragment of a tobacco PR 2 cDNA clone (Ward et al, 1991b), the 1.4-kb EcoRI fragment of a tobacco Pli Q' cDNA (Ward et al, 1991b), the 0.65-kb XhoI-BamHI fragment of the tobacco stylar P-1,3-glucanase cDNA clone sp4la (Ori et al, 1990), and a 1.8-kb EcoRI fragment of genomic DNA encoding tomato 18s ribosomal RNA (Schmidt-Puchta et al, 1989). The probes were labeled with [c~-~'P]~ATP by random priming (Boehringer Mannheirn).…”

Section: Northern Analysismentioning

confidence: 99%

Class I [beta]-1,3-Glucanases in the Endosperm of Tobacco during Germination

Fründt²,

et al. 1995

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Rupture of the seed coat and rupture of the endosperm are separate events in the germination of Nicotiana tabacum 1. cv Havana 425 seeds. Treatment with 1 O-5 M abscisic acid (ABA) did not appreciably affect seed-coat rupture but greatly delayed subsequent endosperm rupture by more than 100 h and resulted in the formation of a novel structure consisting of the enlarging radicle with a sheath of greatly elongated endosperm tissue. Therefore, ABA appears to act primarily by delaying endosperm rupture and radicle emergence. Measurements of P-1,3-glucanase activity, antigen content, and mRNA accumulation together with reporter gene experiments showed that induction of class I P-1,3-glucanase genes begins just prior to the onset of endosperm rupture but after the completion of seed-coat rupture. This induction was localized exclusively in the micropylar region of the endosperm, where the radicle will penetrate. ABA treatment markedly inhibited the rate of /3-1,3-glucanase accumulation but did not delay the onset of induction. lndependent of the ABA concentration used, onset of endosperm rupture was correlated with the same P-1,3-glucanase content/seed. These results suggest that ABA-sensitive class I p-1,3-glucanases promote radicle penetration of the endosperm, which is a key limiting step in tobacco seed germination.

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Cloning and sequence analysis of the 18 S ribosomal RNA gene of tomato and a secondary structure model for the 18 S rRNA of angiosperms

Cited by 17 publications

References 40 publications

Sequence of 18S rDNA of actinorhizal Alnus glutinosa (Betulaceae)

Sequence of 18S rDNA of actinorhizal Alnus glutinosa (Betulaceae)

Characterization of Source- and Sink-Specific Sucrose/H+ Symporters from Carrot

Class I [beta]-1,3-Glucanases in the Endosperm of Tobacco during Germination

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