Cloning and functional identification of two members of the ZIP (Zrt, Irt-like protein) gene family in rice (Oryza sativa L.)

Abstract

Two ZIP (Zrt, Irt-like Protein) cDNAs were isolated from rice (Oryza sativa L.) by RT-PCR approach, and named as OsZIP7a and OsZIP8 respectively. The predicted proteins of OsZIP7a and OsZIP8 consist of 384 and 390 amino acid residues respectively, and display high similarity to other plant ZIP proteins. Each protein contains eight transmembrane (TM) domains and a highly conserved ZIP signature motif, with a histidine-rich region in the variable region between TM domains III and IV. By semi-quantitative RT-PCR approach, it was found that the expression of OsZIP7a was significantly induced in rice roots by iron-deficiency, while that of OsZIP8 induced in both rice roots and shoots by zinc-deficiency. When expressed in yeast cells, OsZIP7a and OsZIP8 could complement an iron-uptake-deficient yeast mutant and a zinc-uptake-deficient yeast mutant respectively. It suggested that the OsZIP7a and OsZIP8 might encode an iron and a zinc transporter protein in rice respectively.

Gene limiting cadmium accumulation in rice

“…Among them, Os07g0232800 and Os07g0232900 encode putative heavy-metal transporters, OsZIP8 and OsHMA3, respectively. OsZIP8 is reported to encode an influx transporter of zinc (12). At first, we isolated the full-length cDNA of OsZIP8 from Anjana Dhan (OsZIP8a) and Nipponbare (OsZIP8n).…”

Mapping QTLs for mineral element contents in brown and milled rice using anOryza sativa×O. rufipogonbackcross inbred line population

“…The OsZIP3 gene was located in the qZn4 region, which was expressed in roots and responsible for Zn uptake from soil (Yang et al 2009). In the qZn6 and qMn6 interval, the OsNRAMP3 gene was constitutively expressed in the node and transported Mn to young leaves and panicles (Yang et al 2013).…”

Zn Uptake and Translocation in Rice Plants

“…1; Ishimaru et al 2006;Lee et al 2010;Ramesh et al 2003;Yang et al 2009). OsZIP1, OsZIP3, OsZIP4, and OsZIP5 were found to be rice Zn transporters induced by Zn deficiency (Ramesh et al 2003;Lee et al 2010;Ishimaru et al 2006).…”