volume 164, issue 3, P983-993 1985
DOI: 10.1128/jb.164.3.983-993.1985
View full text
Has correction 2021-02-02
|
|
Share

Abstract: The citrate utilization determinant from a large 200-kilobase (kb) naturally occurring plasmid was previously cloned into the PstI site of plasmid vector pBR325 creating the Cit+ tetracycline resistance plasmid pWR61 (15 kb). TnS insertion mutagenesis analysis of plasmid pWR61 limited the segment responsible for citrate utilization to a 4.8-kb region bordered by EcoRI and PstI restriction nuclease sites. The 4.8-kb fragment was cloned into phage M13, and the DNA'sequence was determined by the dideoxyribonucleo…

Expand abstract
Editorial notices