Cloning and characterization of sbr: a new colonial mutant of Neurospora crassa.

Trans-species expression of a nonself recognition domain

“…We also employed the small brown locus (SBR) as a standard across qPCR reactions. The SBR locus encodes a putative regulatory element that may be involved in tubulin distribution (Campsall, 1998). We reasoned that expression of this locus would not be affected by incompatibility and indeed, qPCR data with primers specific to the SBR locus demonstrated that there was no significant difference between pre-and post-escape SBR cDNA concentrations (P = 0.98, Figure 3.6).…”

“…(denaturation), 30 s of annealing at temperatures indicated in Table 3.1 and 30 s at 72°C (extension). To verify that equal amounts of cDNA were added to each reaction mix, we performed qPCR using primers Sbr-yy9 and sbrL-02RC that were specific to the SBR locus (Campsall, 1998).…”