Cloning and characterization of salicylic acid-induced, intracellular pathogenesis-related gene from tomato (Lycopersicon esculentum)

Abstract

Intracellular pathogenesis-related gene (IPR) from tomato was cloned from a salicylic acid (SA) induced cDNA library and was designated as TSI-1 (tomato stress induced-1). The deduced amino acid sequence of TSI-1 codes for a 178 amino acid polypeptide of molecular weight 20?4 kDa. TSI-1 is highly homologous to the potato STH-2 and STH-21 IPR and tree pollen allergens which cause type I allergic reactions in humans. TSI-1 lacks a signal peptide like other IPR members. It is organized as a multigene family and is inducible by SA and Fusarium oxysporum infection.

Reduced allergenicity of tomato fruits harvested from Lyc e 1–silenced transgenic tomato plants

“…Residual IgE reactivity is most likely due to other tomato allergens, such as Lyc e 2 (b-fructofuranosidase), Lyc e 3 (nonspecific lipid transfer protein [LTP]), polygalacturonase, pectinesterase, chitinase, superoxide dismutase, pathogenesis-related protein 10, and thus far unknown allergens. [5][6][7][8][9][10] In patients with tomato allergy and multiple sensitizations to other food sources and pollen, IgE directed to Lyc e 1 showed a strong cross-reactivity with profilin from birch pollen, Bet v 2, and a weaker but significant cross-reactivity with profilins from celery, carrot, cherry, and pineapple. 3 IgE directed against tomato profilin can be responsible for cross-reactivity between unrelated plant sources, but IgE epitope differences within the profilin family exist.…”

Xanthomonas T3S Effector XopN Suppresses PAMP-Triggered Immunity and Interacts with a Tomato Atypical Receptor-Like Kinase and TFT1

“…PR-Q'b encodes a class III b-1,3-glucanase (Domingo et al, 1994). Tsi-1 encodes the tomato homolog of the potato (Solanum tuberosum) STH-2 gene (Sree Vidya et al, 1999). GST encodes glutathione S-transferase (Harvey et al, 2008).…”

Transcriptome analysis reveals novel genes involved in nonhost response to bacterial infection in tobacco

“…The TDF 45C14-1-7c (8 hpi) showed homology to a potato pathogenesis-related protein (Table 1), also known as PR-10. This protein displays structural homology to the Bet v I allergen family and to proteins whose expression are induced in response to intracellular pathogens, with some showing ribonuclease activity, suggesting a role in the degradation of pathogenic RNA (Sree Vidya et al, 1999). The TDF 41C24-4-4c (8 hpi) showed homology to a class V chitinase/lysozyme from tobacco (Table 1), within the tobacco PR-3 group.…”