Cloning and characterization of ovine immunoglobulin G Fc receptor II (FcγRII)

Liu, Yunchao; Wang, Aiping; Qiao, Songlin; Zhang, Gaiping; Xi, Jun; You, Leiming; Tian, Xingui; Li, Qiaomu; Zhang, Lina; Guo, Junqing

doi:10.1016/j.vetimm.2009.07.020

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…More than one type of FcR is expressed on the surface of PAMs (Liu et al 2010). Both porcine FccRI and FccRIIb receptors play crucial roles in the ADE of PRRSV (Gu et al 2015).…”

Section: Fceri Enhances Viral Replication During Prrsv Infectionmentioning

confidence: 99%

Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

et al. 2018

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Porcine reproductive and respiratory syndrome virus (PRRSV) shows characteristic antibody-dependent enhancement (ADE) of infection and causes porcine systemic inflammation, which is similar to a type I allergic reaction; however, the role of porcine FcεRI in ADE is still unclear. In this study, the expression of different Fc receptors (FcRs) on macrophages was investigated in a PRRSV 3D4/21 cell infection model in the presence or absence of PRRSV antibody. The transcription level of FcγII and FcεRI was significantly up-regulated under PRRSV-antibody complex infection. Internalization and proliferation of PRRSV were promoted by the ADE mechanism when FcεRI was expressed in permissive 3D4/21 cells and the non-permissive cell line HEK 293T. Transcriptome sequencing data showed that the expression levels of AKT, ERK and other signal molecules in the anti-inflammatory pathway were significantly increased, especially in the cells infected with the PRRSV-antibody immune complex. Inflammatory regulatory molecules such as PLA2G6, LOX, TRPM8 and TRPM4 were significantly up-regulated following PRRSV infection but significantly down-regulated in the cells infected with the PRRSV-antibody immune complex. Our results demonstrated that FcεRI could be involved in PRRSV ADE, the antigen presenting process and regulation of the inflammatory response during PRRSV infection, which provides new insights into PRRSV infection mediated by FcεRI and the PRRSV-antibody immune complex.

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“…More than one type of FcR is expressed on the surface of PAMs (Liu et al 2010). Both porcine FccRI and FccRIIb receptors play crucial roles in the ADE of PRRSV (Gu et al 2015).…”

Section: Fceri Enhances Viral Replication During Prrsv Infectionmentioning

confidence: 99%

Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

et al. 2018

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Genetic characterization and ligand specificity of the ovine Fc gamma receptor I (ovFcγRІ)

Qiao

Liu

Zhang

et al. 2010

Veterinary Immunology and Immunopathology

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Cloning and characterization of ovine immunoglobulin G Fc receptor III (FcγRIII)

Liu

Qiao

Wang

et al. 2011

Veterinary Immunology and Immunopathology

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Receptors for the Fc regions of immunoglobin G (IgG) play a critical role in immunoregulation and immune defenses against pathogens. In this study, we describe the cloning, eukaryotic expression and IgG subclass specificity of ovine Fc gamma receptor III (FcγRIII). The newly cloned ovine FcγRIII cDNA contains a 940 bp open-reading frame (ORF), and is predicted to encode a 250 amino acid transmembrane glycoprotein composed of two immunoglobulin-like extracellular domains, a transmembrane region and a short cytoplasmic tail. The overall identity of the ovine FcγRIII amino acid sequence to its cattle, pig and human counterparts was 83.2%, 62.0%, 60.7%, respectively. Overlapping PCR was performed with the extracellular domain of ovine FcγRIII and the transmembrane and intracellular region of ovine Fc gamma chain to construct a chimeric receptor. Rosetting analysis showed that transfected COS-7 cells required Fc receptor gamma chain for the expression of FcγRIII on the surface. COS-7 cells expressing FcγRIII were able to bind chicken erythrocytes sensitized with ovine IgG1, but not IgG2. Identification of ovine FcγRIII will further our understanding of the ovine immune system.

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Cloning and characterization of ovine immunoglobulin G Fc receptor II (FcγRII)

Cited by 3 publications

References 23 publications

Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

Genetic characterization and ligand specificity of the ovine Fc gamma receptor I (ovFcγRІ)

Cloning and characterization of ovine immunoglobulin G Fc receptor III (FcγRIII)

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