Cloning and characterization of avasa-like gene in rainbow trout and its expression in the germ cell lineage

Cloning and Expression Profiling of the Gene vasa during First Annual Gonadal Development of Cobia (Rachycentron canadum)

“…This indicated that vasa might have a species-specific role in regulating the transformation of spermatogenic cells. The expression pattern of Rcvasa during oogenesis was similar to those observed in several teleost species [3,18,36] and revealed that Rcvasa mRNA was abundant in early primary oocytes (stage I and II, perinucleolar and previtellogenic stage) and deficient in yolk vesicle (cortical alveolus) formation-stage oocytes (stage III, vitellogenic stage). These findings indicate that vasa might regulate oocyte development during the early stage of oogenesis.…”

Characterization of ddx4 and dnd Homologs in Snakeskin Gourami (Trichopodus pectoralis) and Their Expression Levels during Larval Development and in Gonads of Males and Females

“…Using RT-PCR and in situ hybridization, we demonstrated that ddx4 and dnd1 are specifically expressed in the gonads, particularly in germ cells. Similar specific expressions of ddx4 mRNA in the gonads have been demonstrated in rainbow trout [ 50 ], medaka [ 51 ], tilapia [ 30 ], gibel carp [ 52 ], rice field eel [ 53 ], Pacific bluefin tuna [ 54 ], carp [ 55 ], catfish [ 18 ], European seabass [ 56 ], rare minnow [ 57 ], Atlantic cod [ 58 ], Korean rockfish [ 59 ], Solea senegalensis [ 60 ], toadfish [ 61 ], Japanese flounder [ 20 ], brown-marbled grouper [ 31 ], striped catfish [ 7 ], and Mekong giant catfish [ 62 ]. Coincidentally, the specific expression of dnd1 in testes and ovaries was revealed in zebrafish [ 15 ], medaka [ 29 ], rare minnow [ 63 ], gibel carp [ 13 ], tambaqui [ 64 ], Atlantic salmon [ 65 ], and starry flounder [ 21 ].…”

Characteristics of the Vasa Gene in Silurus asotus and Its Expression Response to Letrozole Treatment

“…It shared similarities with other Vasa homologs within the DEAD-box protein family, exhibiting at least eight characteristic motifs of Vasa proteins [ 40 , 41 ]. Notably, the N-terminal region displayed rich RG and RGG repeats, similar to Vasa homologs in other teleosts such as the grass carp, Drosophila , and the rainbow trout ( Oncorhynchus mykiss ) [ 42 , 43 , 44 ]. As known, there are three subfamilies of Vasa, PL10, and P68 in the DEAD-box protein family.…”