volume 176, issue 23, P7309-7319 1994
DOI: 10.1128/jb.176.23.7309-7319.1994
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Abstract: RP4-mediated transfer of mobilizable plasmids in intergeneric conjugation of Escherichia coli donors with Corynebacterium glutamicum ATCC 13032 is severely affected by a restriction system in the recipient that can be inactivated by a variety of exogenous stress factors. In this study a rapid test procedure based on intergeneric conjugal plasmid transfer that permitted the distinction between restriction-negative and restriction-positive C. gludamicum clones was developed. By using this procedure, clones of t…

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