Cloning and allelic variation of two novel catalase genes (SoCAT-1andSsCAT-1) inSaccharum officinarumL. andSaccharum spontaneumL.

Abstract

(2015) Cloning and allelic variation of two novel catalase genes (SoCAT-1 and SsCAT-1) in Saccharumofficinarum L. and Saccharumspontaneum L., Biotechnology & Biotechnological Equipment, 29:3, 431-440, DOI: 10.1080/13102818.2015 Catalases (CAT) play important roles in plant defence mechanisms, stress response, cellular redox balance and aging delay. In order to clone CAT genes in cultivated (Saccharum officinarum L.) and wild (S. spontaneum L.) sugarcane, we designed two pairs of specific primers using a full length cDNA sequence of sorghum CAT gene (XM 002437586.1) as the probe. Two novel full-length sequences of CAT genes (SoCAT-1 in S. officinarum and SsCAT-1 in S. spontaneum) were cloned by nested polymerase chain reaction. Two allelic variants were found at SoCAT-1 (SoCAT-1a and SoCAT-1b, GenBank accession nos. KF864224-25) and SsCAT-1 (SsCAT-1a and SsCAT-1b, GenBank accession nos. KF864226-27) loci. The full lengths of SoCAT-1a, SoCAT-1b, SsCAT-1a and SsCAT-1b were 3816 bp, 3814 bp, 3777 bp and 3812 bp, respectively, and all contained eight exons and seven introns, with a similar gene structure. The length of cDNA for all four genes was 1532 bp, containing 10 bp 5'-untranslated region (UTR) and 43 bp 3'-UTR, and an open reading frame of 1479 bp encoding a polypeptide of 492 amino acids. High homology of DNA (99.0% for SoCAT-1, 98.2% for SsCAT-1) and cDNA (99.3% for SoCAT-1, 99.4% for SsCAT-1) were found. Eleven single nucleotide polymorphisms were found in cDNA SoCAT-1 and nine ones in SsCAT-1, and nine and five amino-acid mutation sites in the predicted proteins, respectively. The predicted proteins encoded by SoCAT-1 and SsCAT-1 showed high homology with CAT in sorghum, rice, corn and other species.

CsCAT3, a catalase gene fromCucumis sativus, confers resistance to a variety of stresses toEscherichia coli

“…The protein sequence of CsCAT3 was aligned with the template protein 4qol.1.A from amino acid 17-448 with a coverage of 95%. As shown in Figure 2, the predicted structure of CsCAT3 could be conceptually divided into four subunits, which is in accordance with a pervious study [14]. In addition, The CsCAT3 protein and template protein shared a high degree of homology (49.04%), and the GMQE value and QMEAN Z-Score were 0.77 and ¡1.08, respectively, suggesting a high reliability of the predicted structure ( Figure 2).…”

“…Genomewide studies have demonstrated that CAT is encoded by a small gene family which has been reported in many plant species, including Nicotiana plumbaginifolia [4], Hordeum vulgare [5], Zea mays [6], Arabidopsis thaliana [7,8], Solanum lycopersicum [9], Capsicum annuum [10], Oryza sativa [11,12], Saccharum officinarum [13,14 ], and Cucumis sativus [15].…”

Catalase and ascorbate peroxidase—representative H2O2-detoxifying heme enzymes in plants

“…Catalase (H 2 O 2 /H 2 O 2 oxidoreductase, EC 1.11.1.6; CAT) is a tetrameric, heme-containing enzyme that catalyzes the dismutation of H 2 O 2 into H 2 O and O 2 and plays important role not only in plant metabolism and defense but also in signal perception (Loew 1901;Redinbaugh et al 1990;Scandalios et al 1997;Mhamdi et al 2010;Hu et al 2010;Nie et al 2015;Liu et al 2015). Historically, the degradation of H 2 O 2 in plant and animal tissues was first observed by Thenard in 1881; whereas, Loew (1901) proved that a new enzyme, which he named Bcatalase,ŵ as responsible for the degradation of H 2 O 2 in tissues (Aebi and Sutter 1971).…”

“…Regarding CAT genes and isoforms, CATs can exist in multiple molecular forms or isozymes encoded by multiple genes (Scandalios 1968). Notably, unlike animals which contain a single CAT gene, all angiosperm/flowering plants including Oryza sp., Arabidopsis, Zea mays, Nicitiana tabacum, and pumpkin are composed of a multigene family (Scandalios 1990;Frugoli et al 1996;Guan and Scandalios 1996;Iwamoto et al 2000; reviewed by Mhamdi et al 2010;Liu et al 2015). Multiple forms of CATs in plants are usually expressed in different tissues and at their developmental stages (reviewed by Scandalios et al 1997).…”

Oxidative Stress, Ageing and Methods of Seed Invigoration: An Overview and Perspectives

“…They are recognised as essential defence enzymes against ROS-induced oxidative stress [154,155]. In addition, they are involved in plant defence and metabolism as well as the perception of cellular signals [156].…”