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“…If an air puff of sufficient strength is directed at the animal's face when it is immobilized by clamping the neck, the animal is able to move away from the innocuous stimulus (Fleischmann and Urca, 1988b). Moreover, neurophysiologic evidence shows that the neck pinch inhibits spinal neural activity caused by noxious peripheral stimulation, whereas the response of spinal neurons to light touch or proprioception is not attenuated (Fleischmann and Urca, 1989). In the present study, repeated handling and clamping of mice could reduce the time to score the writhing response, and confound the antinociception tests.…”
Section: Discussionmentioning
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“…If an air puff of sufficient strength is directed at the animal's face when it is immobilized by clamping the neck, the animal is able to move away from the innocuous stimulus (Fleischmann and Urca, 1988b). Moreover, neurophysiologic evidence shows that the neck pinch inhibits spinal neural activity caused by noxious peripheral stimulation, whereas the response of spinal neurons to light touch or proprioception is not attenuated (Fleischmann and Urca, 1989). In the present study, repeated handling and clamping of mice could reduce the time to score the writhing response, and confound the antinociception tests.…”
Section: Discussionmentioning
“…Mice were pinched and then clamped on the scruff of the neck with a 5-cm serrated alligator clip exerting 1500 g of force (Fleischmann and Urca, 1989). The tips were covered with masking tape to avoid any injury to the mouse's skin.…”
Section: Immobility Testingmentioning
“…In the rat [13][21], the mouse [22], the cat [23], [24] and the monkey [25], [26], most wide-dynamic-range and some nociceptive-specific neurons in the dorsal horn are strongly inhibited by a noxious stimulus applied outside their excitatory receptive fields. Such effects are not organized somatotopically but apply to the whole body.…”
Section: Discussionmentioning