Clinical Specular Microscopy

Morphological analysis of the corneal endothelium in eyes of dogs using specular microscopy

“…Rapid quantitative analysis regard the state of the corneal endothelium has been cited as another advantage of this method (Laing et al 1979). Specular microscopy may be either a contact microscope, in which the front of the objective lens touches the cornea, or a non contact specular microscope, in which there is an air space between the front of objective lens and the cornea.…”

“…The polymegathism is an index that provides a quantitative measurement of variation in cell size and the pleomorphism is the variation of cell shape such as percentage of hexagonal cells (Laing et al 1979). Despite the relative widespread availability of instruments to the assessment of the endothelial mosaic, there are few detailed reports on endothelial morphology of vertebrates, particularly mammals (Peiffer et al 1981).…”

“…The specular microscopy has become a standard technique to determine endothelial cell counting and cell morphology (Laing et al 1979). Among the useful parameters to determine endothelial cell status are the endothelial cell density and cell morphology.…”

“…The utilization of eyes within 1 h after enucleation allowed the maintenance of endothelial ultrastructure.. Specular microscopy demonstrated that the endothelium was continuous and without detectable abnormalities in all eyes examined. The methods used to analysis of specular images are fixed frame and variable frame analysis (Laing et al 1979). The variable frame method used in our study eliminates the problem of counting fractional cells along the boundary and provided a more accurate determination of parameters evaluated than the fixed frame analysis.…”

Morphological analysis of the corneal endothelium in eyes of dogs using specular microscopy

“…Rapid quantitative analysis regard the state of the corneal endothelium has been cited as another advantage of this method (Laing et al 1979). Specular microscopy may be either a contact microscope, in which the front of the objective lens touches the cornea, or a non contact specular microscope, in which there is an air space between the front of objective lens and the cornea.…”

“…The polymegathism is an index that provides a quantitative measurement of variation in cell size and the pleomorphism is the variation of cell shape such as percentage of hexagonal cells (Laing et al 1979). Despite the relative widespread availability of instruments to the assessment of the endothelial mosaic, there are few detailed reports on endothelial morphology of vertebrates, particularly mammals (Peiffer et al 1981).…”

“…The specular microscopy has become a standard technique to determine endothelial cell counting and cell morphology (Laing et al 1979). Among the useful parameters to determine endothelial cell status are the endothelial cell density and cell morphology.…”

“…The utilization of eyes within 1 h after enucleation allowed the maintenance of endothelial ultrastructure.. Specular microscopy demonstrated that the endothelium was continuous and without detectable abnormalities in all eyes examined. The methods used to analysis of specular images are fixed frame and variable frame analysis (Laing et al 1979). The variable frame method used in our study eliminates the problem of counting fractional cells along the boundary and provided a more accurate determination of parameters evaluated than the fixed frame analysis.…”

Morphology of endothelial cells from different regions of the equine cornea

“…Endothelial parameters that can be quantified principally included mean cell area, cell density, polymegathism and pleomorphism (PIGATTO et al, 2004;PIGATTO et al, 2005b;SELIG et al, 2015). Pleomorphism is the variation in cell shape represented by the percentage of hexagonal cells (LAING et al, 1979). Principal among the techniques used to study the corneal endothelium are specular microscopy and scanning electron microscopy (SEM) (PIGATTO et al, 2005a, PIGATTO et al, 2005bMcCAREY et al, 2008).…”

Morphological analysis of the corneal endothelium in eyes of dogs using specular microscopy

“…Rapid quantitative analysis regard the state of the corneal endothelium has been cited as another advantage of this method (Laing et al 1979). Specular microscopy may be either a contact microscope, in which the front of the objective lens touches the cornea, or a non contact specular microscope, in which there is an air space between the front of objective lens and the cornea.…”

“…The polymegathism is an index that provides a quantitative measurement of variation in cell size and the pleomorphism is the variation of cell shape such as percentage of hexagonal cells (Laing et al 1979). Despite the relative widespread availability of instruments to the assessment of the endothelial mosaic, there are few detailed reports on endothelial morphology of vertebrates, particularly mammals (Peiffer et al 1981).…”

“…The specular microscopy has become a standard technique to determine endothelial cell counting and cell morphology (Laing et al 1979). Among the useful parameters to determine endothelial cell status are the endothelial cell density and cell morphology.…”

“…The utilization of eyes within 1 h after enucleation allowed the maintenance of endothelial ultrastructure.. Specular microscopy demonstrated that the endothelium was continuous and without detectable abnormalities in all eyes examined. The methods used to analysis of specular images are fixed frame and variable frame analysis (Laing et al 1979). The variable frame method used in our study eliminates the problem of counting fractional cells along the boundary and provided a more accurate determination of parameters evaluated than the fixed frame analysis.…”

Morphology of endothelial cells from different regions of the equine cornea

“…Endothelial parameters that can be quantified principally included mean cell area, cell density, polymegathism and pleomorphism (PIGATTO et al, 2004;PIGATTO et al, 2005b;SELIG et al, 2015). Pleomorphism is the variation in cell shape represented by the percentage of hexagonal cells (LAING et al, 1979). Principal among the techniques used to study the corneal endothelium are specular microscopy and scanning electron microscopy (SEM) (PIGATTO et al, 2005a, PIGATTO et al, 2005bMcCAREY et al, 2008).…”

Morphological analysis of the corneal endothelium in eyes of dogs using specular microscopy

“…Rapid quantitative analysis regard the state of the corneal endothelium has been cited as another advantage of this method (Laing et al 1979). Specular microscopy may be either a contact microscope, in which the front of the objective lens touches the cornea, or a non contact specular microscope, in which there is an air space between the front of objective lens and the cornea.…”

“…The polymegathism is an index that provides a quantitative measurement of variation in cell size and the pleomorphism is the variation of cell shape such as percentage of hexagonal cells (Laing et al 1979). Despite the relative widespread availability of instruments to the assessment of the endothelial mosaic, there are few detailed reports on endothelial morphology of vertebrates, particularly mammals (Peiffer et al 1981).…”

“…The specular microscopy has become a standard technique to determine endothelial cell counting and cell morphology (Laing et al 1979). Among the useful parameters to determine endothelial cell status are the endothelial cell density and cell morphology.…”

“…The utilization of eyes within 1 h after enucleation allowed the maintenance of endothelial ultrastructure.. Specular microscopy demonstrated that the endothelium was continuous and without detectable abnormalities in all eyes examined. The methods used to analysis of specular images are fixed frame and variable frame analysis (Laing et al 1979). The variable frame method used in our study eliminates the problem of counting fractional cells along the boundary and provided a more accurate determination of parameters evaluated than the fixed frame analysis.…”

Morphology of endothelial cells from different regions of the equine cornea

“…Endothelial parameters that can be quantified principally included mean cell area, cell density, polymegathism and pleomorphism (PIGATTO et al, 2004;PIGATTO et al, 2005b;SELIG et al, 2015). Pleomorphism is the variation in cell shape represented by the percentage of hexagonal cells (LAING et al, 1979). Principal among the techniques used to study the corneal endothelium are specular microscopy and scanning electron microscopy (SEM) (PIGATTO et al, 2005a, PIGATTO et al, 2005bMcCAREY et al, 2008).…”