Clinical, serological, and molecular evidence of ehrlichiosis and anaplasmosis in dogs in Tunisia

M’ghirbi, Youmna; Ghorbel, A.; Amouri, M.; Nebaoui, A.; Haddad, S.; Bouattour, Ali

doi:10.1007/s00436-008-1253-4

Cited by 51 publications

(29 citation statements)

References 40 publications

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“…In European countries, such as Bulgaria, Spain, Sweden, and Switzerland, seroprevalence of 30% [38], 19% [36], 17.7% [39], and 2.2% [40], respectively, was reported. In Asia, 14.6% has been reported in Iran [11]; in Africa, 67.8% [41] and 54.2% [42] were recorded in Ivory Coast and Tunisia, respectively.…”

Section: Discussionmentioning

confidence: 99%

Associated Factors to Seroprevalence ofEhrlichiaspp. in Dogs of Quintana Roo, Mexico

Martínez-Vega

Bolio-González

Rodríguez-Vivas

et al. 2016

Journal of Tropical Medicine

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The objective of this study was to determine the seroprevalence to Ehrlichia spp. in dogs from Xcalak, Quintana Roo, Mexico, and the associated factors. Serum samples were obtained from 118 dogs and used in an indirect immunofluorescent assay test for the detection of antibodies against Ehrlichia spp. A questionnaire was used to obtain information about possible variables associated with seroprevalence. These variables were analyzed through Chi2 test and logistic regression. Dog seroprevalence of antibodies against Ehrlichia spp. was 64% (75/118). Fifty-two percent (61/118) of dogs had tick infestation which was identified as Rhipicephalus sanguineus sensu lato. Anemia was observed in 36% of dogs. Leucopenia (2.5%), thrombocytopenia (70%), and hemorrhage (14%) were also observed. Thirty-one percent (23/75) of dogs with anemia, 4% (3/75) of dogs with leucopenia, 80% (60/75) of dogs with thrombocytopenia, 17% (13/75) of dogs with hemorrhages, and 59% (44/75) of dogs with ticks were positive for Ehrlichia spp. antibodies. The factors associated with seroprevalence were age (1–3 and >3 years old, OR = 7.77 and OR = 15.39, resp.), tick infestation (OR = 3.13), and thrombocytopenia (OR = 3.36). In conclusion, seroprevalence of Ehrlichia spp. was high in the community of Xcalak and its associated factors were age, tick infestation, and thrombocytopenia.

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Section: Discussionmentioning

confidence: 99%

Associated Factors to Seroprevalence ofEhrlichiaspp. in Dogs of Quintana Roo, Mexico

Martínez-Vega

Bolio-González

Rodríguez-Vivas

et al. 2016

Journal of Tropical Medicine

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“…Until now, molecular findings demonstrated the occurrence of A. phagocytophilum infections in dogs, horses, Ixodes ricinus, Hyalomma scupense, and H. marginale ticks in Tunisia (Sarih et al, 2005;M'ghirbi et al, 2009. A. phagocytophilum was also reported in Tunisian horses and dromedaries by serological method (Ben Said et al, 2014a,b).…”

Section: Introductionmentioning

confidence: 91%

First molecular survey of Anaplasma bovis in small ruminants from Tunisia

Saïd

Belkahia

Karaoud

et al. 2015

Veterinary Microbiology

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“…Moreover, A. phagocytophilum is likely to circulate in a variety of ticks as they feed on dogs or reptiles, as observed in Tunisia, South Africa and Ghana [9,10], although it is unknown whether they can transmit it.…”

Section: Introductionmentioning

confidence: 99%

Anaplasma phagocytophilum in horses and ticks in Tunisia

et al. 2012

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Background Anaplasma phagocytophilum , the causative agent of granulocytic anaplasmosis, affects several species of wild and domesticated mammals, including horses. We used direct and indirect methods to compare and evaluate exposure to A. phagocytophilum in horses in northern Tunisia.MethodsSerum from 60 horses was tested by IFA for antibodies to A. phagocytophilum , and whole blood was tested for A. phagocytophilum 16S rRNA gene using a nested-PCR. To examine the risk of A. phagocytophilum transmission, 154 ticks that had been collected from horses were examined for the presence of A. phagocytophilum by nested-PCR targeting 16S rRNA gene.ResultsThis is the first time that A. phagocytophilum has been detected in horses in Tunisia, with an overall seroprevalence of 40/60 (67%). Six of the seroreactive samples (10%) had an IFA titer of 1:80, 14 (23%) of 1:160, 8 (13%) of 1:320 and 12 (20%) a titer 1 ≥ 640. The seroprevalence revealed no significant regional and sex differences. In contrast, a significant difference was observed between breeds. Eight (13%) of the horses were positive for A. phagocytophilum in the PCR, with no significant breed and age differences. Hyalomma marginatum was a predominant tick species (130/154), and 3 were infected by A. phagocytophilum (a prevalence of 2.3%). The concordance rate of A. phagocytophilum detection between IFA and PCR had a k value of −0.07.ConclusionsThe results presented in this study suggest that horses infested by ticks in Tunisia are exposed to A. phagocytophilum.

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Clinical, serological, and molecular evidence of ehrlichiosis and anaplasmosis in dogs in Tunisia

Cited by 51 publications

References 40 publications

Associated Factors to Seroprevalence ofEhrlichiaspp. in Dogs of Quintana Roo, Mexico

Associated Factors to Seroprevalence ofEhrlichiaspp. in Dogs of Quintana Roo, Mexico

First molecular survey of Anaplasma bovis in small ruminants from Tunisia

Anaplasma phagocytophilum in horses and ticks in Tunisia

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