Clinical picture of uveitis in the presence of ocular hypertension and improvement in disease course with dipeptide carnosine

Mikheytseva, I. M.; Бондаренко, Н. В.; Kolomiichuk, S.; Siroshtanenko, T.

doi:10.31288/oftalmolzh202115561

Cited by 2 publications

(7 citation statements)

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“…Moreover, we have previously reported that carnosine instillation in the conjunctival sac signiticantly reduced neopterin levels in uveal tract tissue, aqueous humor and tear samples from the rabbit eye with experimental anterior uveitis plus OHT, indicating a reduction in inflammation in the anterior segment of the eye [20,44].…”

Section: Discussionmentioning

confidence: 86%

“…We have previously reported that binocular instillation of carnosine solution in the conjunctival sac for 4 weeks resulted in a significant decrease in the severity of inflammation in the anterior and posterior segments, leading to an improved clinical picture with an improvement in uveal metabolism, in eyes of the animals treated for experimental allergic uveitis in the presence of OHT [20].…”

Section: Resultsmentioning

confidence: 98%

“…We have previously found that oxidative stress with a suppressed antioxidative status in the eye is a pathogenetic factor which in the presence of raised IOP contributes to increased severity of experimental uveitis [13,14,20].…”

Section: Resultsmentioning

confidence: 99%

“…Pathogenetic features of ocular inflammatory and degenerative disorders (e.g., non-infectious uveitis complicated by ocular hypertension) indicate that freeradical process activation in the presence of exhaustion of the antioxidative system in uveal tissues is an important trigger of metabolic abnormalities and functional and structural alterations in the eye [11,12,15,20,32].…”

Section: Discussionmentioning

confidence: 99%

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Effects of carnosine on the pro-antioxidative status of the anterior segment in experimental uveitis plus ocular hypertension

Mikheytseva¹,

Bondarenko²,

Kolomiichuk³

2022

Oftalmol Zh

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Background: Finding means for improving the effectiveness of drug therapy of ocular inflammatory disorders, especially those with comorbidities, is a challenge to ophthalmology. Purpose: To assess the efficacy of carnosine for improving the pro/antioxidative imbalance in the anterior segment of rabbit eyes with experimental anterior uveitis in the presence of ocular hypertension (OHT). Material and Methods: Thirty-three rabbits were used in the study. Group 1 (the control group) comprised 9 intact rabbits and group 2, 12 animals with OHT induced prior to experimental non-infectious uveitis. Group 3 comprised 12 animals in which carnosine 5% was administered bilaterally into the conjunctival sac twice daily for four weeks for OHT induced prior to experimental non-infectious uveitis. Non-infectious uveitis was induced by injecting albumin into the anterior chamber, and OHT was induced by a single 0.1-mL injection of carbomer 0.3% into the anterior chamber. Activities of superoxide dismutase, catalase, glutathione peroxidase, NADH-oxidase and xanthine oxidase, and levels of total proteins and malondialdehyde (MDA) and diene conjugate (DC) in uveal tissue (iris and ciliary body) and aqueous humor specimens were determined. Results: Uveal activities of NADH-oxidase and xanthine oxidase were 19.4% (р < 0.05) and 27.2% (р < 0.01), respectively, lower in eyes of rabbits treated with carnosine than in eyes of animals not treated for anterior uveitis plus OHT. Uveal activities of glutathione peroxidase, superoxide dismutase and catalase were 34. 7%, 39.6% and 28.4%, respectively, higher, and uveal MDA and DC levels were 39.3% and 33.3%, respectively, lower, in eyes of the former rabbits than of the latter rabbits. Similar changes were found in aqueous humor specimens of the experimental rabbits. Conclusion: Carnosine, when instilled locally, contributed to improvement in the pro/ antioxidative imbalance in the anterior segment of rabbit eyes with uveitis plus OHT. It is reasonable to include carnosine-based eye drops into multiagent therapy of uveitis complicated by OHT due to their antioxidative, anti-inflammatory and membranestabilizing effects.

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Section: Discussionmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of carnosine on the pro-antioxidative status of the anterior segment in experimental uveitis plus ocular hypertension

Mikheytseva¹,

Bondarenko²,

Kolomiichuk³

2022

Oftalmol Zh

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“…We have previously demonstrated that the course of inflammatory process was significantly more severe in eyes with anterior uveitis with OHT than in eyes with uveitis with normal IOP [15], which was largely due to a more marked activation of enzymes producing active oxygen as well as peroxidative processes in uveal structures of experimental animals [16].…”

Section: Introductionmentioning

confidence: 99%

Neopterin level in the anterior segment of the eye in induced uveitis with ocular hypertension when treated by dipeptide carnosine

Mykheitseva¹,

Si²,

Бондаренко³

et al. 2021

Oftalmol Zh

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Background:We have previously demonstrated that the course of inflammatory process was significantly more severe in eyes with anterior uveitis with ocular hypertension (OHT) than in those with anterior uveitis with normal intraocular pressure (IOP), which was largely due to a more marked activation of enzymes producing active oxygen as well as peroxidative processes in uveal structures of experimental animals. Aside from oxidative stress characteristics, our attention has been drawn to neopterin as a potential marker of inflammation severity and the efficacy of inflammation control. Because there is a paucity of reports focused on the potential for using this characteristic as a diagnostic criterion in ocular disorders, especially in uveitis with raised IOP, studies on this subject are important. Purpose: To assess the impact of dipeptide carnosine on the level of neopterin in the rabbit's anterior eye segment in anterior uveitis with ocular hypertension. Material and Methods: Forty-four Chinchilla rabbits (88 eyes) were divided into 4 experimental groups (group 1, 10 animals with induced OHT only; group 2, 10 animals with induced experimental non-infectious uveitis only; group 3, 12 animals with OHT induced prior to experimental non-infectious uveitis; group 4, 12 animals treated with carnosine for experimental uveitis with OHT). Rabbits of group 4 received 5% carnosine solution into the conjunctival sac, twice daily for the four weeks. The control group comprised 9 intact rabbits. Neopterin enzyme-linked immunosorbent assay kit was used to determine neopterin levels in uveal tract tissue, aqueous humor and tear samples according to the manufacturer's instructions. Statistica 5.5 (StatSoft, Tulsa, OK, USA) software and parametric statistical tests were used for statistical analysis. Results: Neopterin levels in uveal tract tissue, aqueous humor and tear samples taken from rabbit's eyes with induced OHT only were increased compared to controls but lower than those in non-infectious uveitis only. Neopterin levels in uveal tract tissue, aqueous humor and tear samples from rabbit's eyes with induced both anterior uveitis and OHT (group 3) were 5. 2-, 3.7-and 2.4 times higher, respectively, than in controls, and 42.5% (р < 0.05), 28.5% (р < 0.05) and 23.6% (р < 0.05) higher, respectively, than in samples taken from rabbit's eyes of group 2. The carnosine treatment of induced both anterior uveitis and OHT (group 4) contributed to reduced levels of neopterin is samples under study, with neopterin levels in uveal tract tissue, aqueous humor and tear fluid being 39.4%, 38.9% and 30.2% lower, respectively, than in samples taken from rabbit's eyes of group 3 (anterior uveitis plus OHT without treatment) (р < 0.01), and 3.2-, 2.2-and 1.7 times higher, respectively, than in controls (р < 0.001). Conclusion: High neopterin levels in the uveal tract tissues and anterior chamber aqueous fluid indicated its impact on the course of inflammation in animals with induced uveitis only and especially in those with induced both uveit...

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Clinical picture of uveitis in the presence of ocular hypertension and improvement in disease course with dipeptide carnosine

Cited by 2 publications

References 0 publications

Effects of carnosine on the pro-antioxidative status of the anterior segment in experimental uveitis plus ocular hypertension

Effects of carnosine on the pro-antioxidative status of the anterior segment in experimental uveitis plus ocular hypertension

Neopterin level in the anterior segment of the eye in induced uveitis with ocular hypertension when treated by dipeptide carnosine

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