Clinical immunology Pro-inflammatory cytokines for evaluation of inflammatory status in endometriosis

Kang

Molecular Reproduction Devel

et al. 2019

Studies have shown that aberrant expression of IL‐12p40, which is encoded by the interleukin‐12B (IL‐12B) gene, may be involved in the development of endometriosis. In this study, we investigated the role of aberrant methylation of the IL‐12B promoter region and its associated expression in the development of ovarian endometriosis. By using pyrosequencing, we analyzed the methylation level of the IL‐12B promoter region in eutopic and ectopic endometrium of patients with ovarian endometriosis and normal endometrium of control women. The expression of IL‐12B mRNA was detected by quantitative real‐time PCR. The results showed that the methylation level of the IL‐12B promoter region in ectopic and eutopic endometrium of patients with ovarian endometriosis was significantly lower than that in endometrium of women without endometriosis ( p < 0.001 and p = 0.041, respectively). In contrast, mRNA levels were significantly increased in ectopic and eutopic endometrium of patients with ovarian endometriosis compared to those in endometrium of women without endometriosis ( p < 0.001 and p = 0.042, respectively). Correlation analysis showed that the methylation level of the IL‐12B promoter region was negatively correlated with mRNA levels of IL‐12B ( p < 0.001). Our data suggested that aberrant methylation of the IL‐12B promoter region may be responsible for aberrant IL‐12B mRNA expression in endometrium tissue of women, which may be associated with the development of ovarian endometriosis in northern Chinese women.

mentioning

confidence: 87%

Aberrant methylation of the IL‐12B promotor region contributes to the risk of developing ovarian endometriosis

Kang

Molecular Reproduction Devel

et al. 2019

Fundamemntal Clinical Pharma

“…The increased serum levels of the same markers TNF‐α are a cytokine with powerful inflammatory, cytotoxic, and angiogenic effects that has a key role in the initiation of the cascade of numerous inflammatory cytokines and other inflammatory factors. In addition, TNF‐α has been shown to play a pivotal role in the pathology of endometriosis . In our study, TNF‐α was investigated both in serum and in peritoneal fluid.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we investigated the effectivity of alpha-lipoic acid in the treatment of endometriosis, which is an antioxidant and anti-inflammatory drug that has never been experimented in the treatment of endometriosis. In previous studies, increased oxidative stress parameters have been found both in ectopic endometrial tissues and in peripheral blood samples in endometriosis [26][27][28]. Oxidative stress is one of the factors in the pathophysiology of endometriosis.…”

Section: Discussionmentioning

confidence: 99%

Effect of alpha‐lipoic acid on endometrial implants in an experimental rat model

Pınar

Karapınar

Özcan

et al. 2017

To investigate the antioxidant and anti-inflammatory effects of alpha-lipoic acid (ALA) in the treatment of endometriosis in an experimental rat model by evaluating biochemical and histopathologic parameters. Experimental endometriosis was induced by the peritoneal implantation of autologous endometrial tissue. The rats were randomly divided into two groups with eight rats each. Group I was intraperitoneally administered ALA 100 mg/kg/day for 14 days. Group II was intraperitoneally administered saline solution at the same dosage and over the same period. Endometrial implant volume was measured in both groups both pre- and post-treatment. Tumor necrosis factor alpha (TNF-α) was measured in peritoneal fluid. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were assessed in serum. The implants were histopathologically evaluated. In the ALA group, the serum TOS and OSI levels, the endometrial implant volumes, the TNF-α levels in serum and peritoneal fluid, and the histopathologic scores were significantly lower compared to the control group (P < 0.05). Alpha-lipoic acid may have a therapeutic potential in the treatment of endometriosis due to its antioxidant and anti-inflammatory effects.

“…Hypoxia and PPARγ deletion induce inflammatory injuries in ECs, while endometriosis presents with high level of pro-inflammatory cytokines for stimulating ECs (27–29). Accordingly, hypoxia, endometriosis, PPARγ deletion were classified into inflammatory condition.…”

Section: Discussionmentioning

confidence: 99%

Analysis for Carom complex signaling and function by database mining

Liu¹,

Xiong²,

Thomas³

et al. 2016

Front Biosci

Carom is a novel protein that regulates membrane curvature and transmits pathophysiological signaling. The tissue expression of Carom is unclear and its functional role and signaling are unknown. We employed a group of combined database mining strategies and established a working model of Carom signaling. We identified 26 Carom partners and established their expression profiles in human and mouse tissues. We classified three tiers of tissues for Carom/partner expression and found lymph node was the tier 1 tissue expressing Carom and most of its partners. Using GEO database, we discovered that four conditions (hypoxia, endometriosis, PPARγ deletion and iPSC reprogramming) altered Carom/partner expression in endothelial cells. We identified 26 Carom partner signalings by Ingenuity pathway analysis. Ten of the 26 pathways and three genes (ITSN1, UBC and HSPA5) were reported to be regulated in the above four conditions. Paired induction of Carom/ITSN1 elevation was associated with pathological angiogenesis. Whereas, paired reduction of Carom/HSPA5 or UBC was associated with iPSC generation. These results provide an insight on identifying Carom complex model and predicting its functional implications.