In recent years, pharmacotherapy has been developing both by creating new compositions and by more rationally using the existing drugs. In this context, it is important to investigate biotransformation processes in the organism that lead to a decrease in the concentration of a given drug and to the appearance of drug metabolites that may possess different properties. These problems are of special value with respect to drugs used for infusion narcosis and post-operation anesthesia.Analytical tasks pertaining to evaluation of the purity and stability of drags, investigation of their pharmacokinetics and metabolism, and forensic medical examination of substances are now solved using a wide variety of chromatographic techniques, including thin-layer chromatography (TLC), gas chromatography (GC), high-performance liquid chromatography (HPLC), and their combinations with mass spectrometry (MS) such as GC-MS and HPLC-MS.Methods employed for the chromatographic determination of general-purpose anesthetics (fentanyl, ketamine, thiopental) and their metabolites were recently reviewed in [1]. The purpose of this paper was to summarize the experi-