Clinical evaluation of a dried blood spot method for determination of mycophenolic acid in renal transplant patients

Therapeutic drug monitoring of tacrolimus and mycophenolic acid in outpatient renal transplant recipients using a volumetric dried blood spot sampling device

“… and Arpini et al . , who reported ratios of 0.77 ( n = 38) and 0.61 ( n = 77), respectively. Although the proposed conversion factors for both tacrolimus and mycophenolic acid seem plausible, as based on the present study, their considerable variability calls for further exploration, optimization and external validation.…”

A comprehensive review of the published assays for the quantitation of the immunosuppressant drug mycophenolic acid and its glucuronidated metabolites in biological fluids

“…The paired DBS and plasma sample comparison revealed a lower concentration value for MPA in DBS because MPA is predominantly retained in the plasma. However, when the dilution effect in DBS was corrected by the inclusion of a hematocrit factor, the two values of MPA were comparable (Arpini et al, 2013).…”

“…Use of dried blood spot and plasma spot assays for MPA Dried blood spot (DBS) assays are advantageous in resourcelimited regions because they offer flexible home collection, ease of transport without refrigeration and without the need for technical help or expensive instrumentation (Edelbroek et al, 2009). Arpini et al (2013 have explored the applicability of DBS assay for quantification of MPA in renal transplant patients. A standard 6 mm punched DBS was subjected to extraction and quantification for MPA in comparison to the routine plasma extraction /quantification of MPA using similar UHPLC conditions (column chromatography, mobile phase and detection).…”

Ultra-high performance liquid chromatography tandem mass spectrometric method for the determination of tamoxifen, N -desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in dried blood spots—Development, validation and clinical application during breast cancer adjuvant therapy

“…To obtain these conventional samples, it is necessary to perform a phlebotomy in a proper facility, also considering pharmacokinetically appropriated collection times. A novel option for therapeutic drug monitoring that has been emerging recently is testing in dried blood spots on paper (DBS), collected by finger prick [15][16][17], mainly due to the stabilization of the analytes by drying and the possibility of postal transportation, once DBS samples are usually non-bio hazardous. Also, this novel matrix allows the possibility of training patients to take their own samples and flexible collection times, more adequate to their personal posology [18].…”