Clinical chorioamnionitis at term: the amniotic fluid fatty acyl lipidome

Maddipati, Krishna Rao; Romero, Roberto; Chaiworapongsa, Tinnakorn; Chaemsaithong, Piya; Zhou, Shihao; Tarca, Adi L.; Kusanovic, Juan Pedro; Gomez, Ricardo; Docheva, Nikolina; Honn, Kenneth V.

doi:10.1194/jlr.p069096

Cited by 43 publications

(40 citation statements)

References 65 publications

(66 reference statements)

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“…Samples (adjusted to a maximum volume of 1-2 ml) were spiked with a mixture of internal standards consisting of 15(S)-HETE-d 8 , Leukotriene B 4 -d 4 , Resolvin D2-d 5 , 14(15)-EpETrE-d 11 , and prostaglandin E 1 -d 4 (5 ng each) for recovery and quantitation, and mixed thoroughly. The samples were then extracted for PUFA metabolites using C18 extraction columns, as described earlier [3][4][5][6] . Briefly, the internal standard spiked samples were applied to conditioned C18 cartridges, washed with water followed by hexane and dried under vacuum.…”

Section: Lc-ms/ms and Lipidomic Analysismentioning

confidence: 99%

“…Lipids were extracted from 1x10 6 cells with minor modification of Folch method 12 . Briefly, cells were homogenized with 500 µl of chloroform/methanol 1:2, centrifuged to recover the lipid extract and extracted again two times with chloroform/methanol 2:1, 1:1 (v/v).…”

Section: Gas Chromatography (Gc) Fatty Acid Analysismentioning

confidence: 99%

“…Indeed, only recently the resolution of inflammation has been conclusively recognized as an active process orchestrated by resolving lipid mediators, which dampen inflammation-sustaining events, such as angiogenesis, release of inflammatory cytokines, clearance of apoptotic cells and microorganisms 4,5 . Among these resolving molecules, those derived from essential ω-3-polyunsaturated fatty acids (PUFAs), have been shown to exert beneficial effects on a wide range of human inflammatory disorders, including UC 6 . In particular, epoxy metabolites of docosahexaenoic acid (DHA), generated by cytochrome P-450 (CYP450) enzymes, promote potent biological activities in the regulation of vascular, pulmonary, cardiac and intestinal functions 7,8 .…”

Section: Introductionmentioning

confidence: 99%

“…Although the endothelium plays a major role in resolution of inflammation, the exact mechanisms behind this process are still unknown, particularly in the gastrointestinal tract. The endothelial lysophosphatidylcholine transporter, namely the major facilitator superfamily domain M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 6 containing 2A (MFSD2A), has recently been shown to be essential for DHA uptake in the blood brain barrier, eye and placenta [12][13][14] . Here we elucidate a significant role for MFSD2A in the maintenance of the balance between inflammatory and resolving epoxy metabolites of DHA in intestinal microvasculature and how this could be used for the development of novel therapeutic strategies to treat UC.…”

Section: Introductionmentioning

confidence: 99%

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MFSD2A Promotes Endothelial Generation of Inflammation-Resolving Lipid Mediators and Reduces Colitis in Mice

et al. 2017

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Section: Lc-ms/ms and Lipidomic Analysismentioning

confidence: 99%

Section: Gas Chromatography (Gc) Fatty Acid Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

MFSD2A Promotes Endothelial Generation of Inflammation-Resolving Lipid Mediators and Reduces Colitis in Mice

et al. 2017

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“…The soluble components include carbohydrates, proteins, peptides, lipids, lactate, pyruvate, electrolytes, enzymes, and hormones, among others 81–99 , many of which act as the first line of defense against pathogens invading the amniotic cavity 100–106 . The cellular components of the amniotic fluid include different cell types derived from exfoliating surfaces of the developing fetus, including the skin, respiratory system, urinary tract, and gastrointestinal tract, as well as stem cells 107–115 .…”

Section: Introductionmentioning

confidence: 99%

The immunophenotype of amniotic fluid leukocytes in normal and complicated pregnancies

Gomez‐Lopez

Romero

et al. 2018

American J Rep Immunol

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Problem The immune cellular composition of amniotic fluid is poorly understood. Herein, we determined the immunophenotype of amniotic fluid: 1) immune cells during the second and third trimester; 2) T cells and innate lymphoid cells (ILCs); and 3) immune cells during intra-amniotic infection/inflammation. Method of Study Amniotic fluid samples (n=57) were collected from women from 15-40 weeks of gestation without intra-amniotic infection/inflammation. Samples from women with intra-amniotic infection/inflammation were also included (n=9). Peripheral blood mononuclear cells from healthy adults were used as controls (n=3). Immunophenotyping was performed using flow cytometry. Results In the absence of intra-amniotic infection/inflammation, the amniotic fluid contained several immune cell populations from 15-40 weeks. Among these immune cells: 1) T cells and ILCs were greater than B cells and NK cells between 15 to 30 weeks; 2) T cells were most abundant between 15 to 30 weeks; 3) ILCs were most abundant between 15 to 20 weeks; 4) B cells were scarce between 15 to 20 weeks; yet, they increased and were constant after 20 weeks; 5) NK cells were greater between 15 to 30 weeks than at term; 6) ILCs expressed high levels of RORγt, CD161, and CD103 (i.e. Group 3 ILCs); 7) T cells expressed high levels of RORγt; 8) neutrophils increased as gestation progressed; and 9) monocytes/macrophages emerged after 20 weeks and remained constant until term. All of the amniotic fluid immune cells, except ILCs, were increased in the presence of intra-amniotic infection/inflammation. Conclusions The amniotic fluid harbors a diverse immune cellular composition during normal and complicated pregnancies.

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Linoleic Acid‐Derived Oxylipins Differentiate Early Stage Alcoholic Hepatitis From Mild Alcohol‐Associated Liver Injury

et al. 2021

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Alcohol-associated liver disease (ALD) is a spectrum of liver disorders ranging from steatosis to steatohepatitis, fibrosis, and cirrhosis. Alcohol-associated hepatitis (AH) is an acute and often severe form of ALD with substantial morbidity and mortality. The mechanisms and mediators of ALD progression and severity are not well understood, and effective therapeutic options are limited. Various bioactive lipid mediators have recently emerged as important factors in ALD pathogenesis. The current study aimed to examine alterations in linoleic acid (LA)-derived lipid metabolites in the plasma of individuals who are heavy drinkers and to evaluate associations between these molecules and markers of liver injury and systemic inflammation. Analysis of plasma LA-derived metabolites was performed on 66 individuals who were heavy drinkers and 29 socially drinking but otherwise healthy volunteers. Based on plasma alanine aminotransferase (ALT) levels, 15 patients had no liver injury (ALT ≤ 40 U/L), 33 patients had mild liver injury (ALT > 40 U/L), and 18 were diagnosed with moderate AH (mAH) (Model for End-Stage Liver Disease score <20). Lipoxygenasederived LA metabolites (13-hydroxy-octadecadienoic acid [13-HODE] and 13-oxo-octadecadienoic acid) were markedly elevated only in patients with mAH. The cytochrome P450-derived LA epoxides 9,10-epoxy-octadecenoic acid (9,10-EpOME) and 12,13-EpOME were decreased in all patients regardless of the presence or absence of liver injury. LA-derived diols 9,10-dihydroxy-octadecenoic acid (9,10-DiHOME) and 12,13-DiHOME as well as the corresponding diol/epoxide ratio were elevated in the mAH group, specifically compared to patients with mild liver injury. We found that 13-HODE and 12,13-EpOME (elevated and decreased, respectively) in combination with elevated interleukin-1β as independent predictors can effectively predict altered liver function as defined by elevated bilirubin levels. Conclusion: Specific changes in LA metabolites in individuals who are heavy drinkers can distinguish individuals with mAH from those with mild ALD. (Hepatology Communications 2021;0:1-14). L ong-term heavy alcohol consumption results in the development of a spectrum of liver disorders ranging from steatosis to steatohepatitis with varying degrees of fibrosis and cirrhosis, collectively known as alcohol-associated liver disease (ALD). ALD affects millions of patients worldwide

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Clinical chorioamnionitis at term: the amniotic fluid fatty acyl lipidome

Cited by 43 publications

References 65 publications

MFSD2A Promotes Endothelial Generation of Inflammation-Resolving Lipid Mediators and Reduces Colitis in Mice

MFSD2A Promotes Endothelial Generation of Inflammation-Resolving Lipid Mediators and Reduces Colitis in Mice

The immunophenotype of amniotic fluid leukocytes in normal and complicated pregnancies

Linoleic Acid‐Derived Oxylipins Differentiate Early Stage Alcoholic Hepatitis From Mild Alcohol‐Associated Liver Injury

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