Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus.

Monkeypox

“…Environmental contamination with monkeypox virus DNA in both the household and patient-care environment of people with monkeypox has been reported, including detection of replication-competent virus samples. 85 , 86 , 87 Investigations in respiratory isolation rooms of a hospital found viral DNA in rooms, bathrooms, anterooms, health-care workers' personal protective equipment, and non-touch surfaces (eg, >1·5 m from the bed). 88 It is unclear whether indirect contact with fomites is a frequent transmission pathway; however, the widespread surface contamination of the patient-care environment calls for a systematic approach to surface cleaning and appropriate use of personal protective equipment by health-care workers.…”

Persistence of monkeypox virus DNA in clinical specimens

“…Pettke et al reported positive PCR results in semen samples from patients who recovered from MPX for 54 d at maximum [3] . Nörz et al found the prolonged presence of MPXV DNA in rash or skin lesions and blood samples [4] . In this study, we estimated the time for MPX cases to clear MPXV DNA in the acute phase of infection through an AFT-based modeling study.…”

What should we do about monkeypox?

“…The fourth suggestion is that novel diagnostics, vaccines, and therapeutics are still immediately needed. Although several detection methods have been reported and validated, 11,12 Norz et al found that the qPCR results from different specimen types in ambulatory and hospitalized patients infected with MPXV are various 13 . The positive rate of qPCR in detecting lesion swabs caused by MPXV is reliable 13 .…”

“…Although several detection methods have been reported and validated, 11,12 Norz et al found that the qPCR results from different specimen types in ambulatory and hospitalized patients infected with MPXV are various 13 . The positive rate of qPCR in detecting lesion swabs caused by MPXV is reliable 13 . In contrast, the false negative rates of qPCR in oropharyngeal swabs and blood samples are high, 13 which may interfere with the diagnosis of the subclinical or asymptotic samples.…”

“…The positive rate of qPCR in detecting lesion swabs caused by MPXV is reliable 13 . In contrast, the false negative rates of qPCR in oropharyngeal swabs and blood samples are high, 13 which may interfere with the diagnosis of the subclinical or asymptotic samples. Thus, a reliable method for MPXV diagnosis using multiple tissues or swabs is immediately needed.…”