Click modification of helical amylose by poly( l -lysine) dendrons for non-viral gene delivery

Pang, Jiangli; Zhuang, Baoxiong; Mai, Kaijin; Chen, Rufu; Wang, Jie; Zhang, Liming

doi:10.1016/j.msec.2015.01.011

Cited by 15 publications

(11 citation statements)

References 23 publications

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“…In recent years, there has been a growing interest in the chemical modification of naturally occurring polysaccharides for developing non-viral gene vectors (Pang et al 2015). Chitosan has been widely investigated for its cationic behavior and ability to bind to negatively charged molecules such as DNA, RNA, and oligonucleotide.…”

Section: Chitosan-based Biomaterialsmentioning

confidence: 99%

"Click" chemistry as a tool to create novel biomaterials: a short review

Barbosa

Martins

Gomes

2017

UPjeng

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In recent years, there has been a growing demand for novel strategies for biomedical applications. Chitosan is a typical cationic amino-containing polysaccharide that has been widely used due to its unique properties. The grafting modification of chitosan has been explored as an interesting method to develop multifunctional novel chitosan hybrid materials for drug delivery, tissue engineering, and other biomedical applications. Recently, “click” chemistry has been introduced into the synthesis of polymeric materials with well-defined and complex chain architectures. The Huisgen’s 1,3-dipolar cycloaddition reaction between alkynes and azides yielding triazoles is the principal example of a “click” reaction. Bioconjugation, surface modification, and orthogonal functionalization of polymers were successfully performed through this chemoselective reaction. In recent literature interest has been shown in this cycloaddition for the modification of polysaccharides, however, only a few chitosan graft copolymers have been synthesized by this technique.

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Section: Chitosan-based Biomaterialsmentioning

confidence: 99%

"Click" chemistry as a tool to create novel biomaterials: a short review

Barbosa

Martins

Gomes

2017

UPjeng

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“…Because of facile availability from nature, renewability, biodegradability, and easy functionalization, polysaccharide‐based materials are widely applied in the fields of textile, [ 1–3 ] drug delivery, [ 4–6 ] metal recovery, [ 7,8 ] and separation science, [ 9–12 ] and so on. Chitosan exhibits antibacterial, hemostasis, and facilitates delivery of drug and gene into a cell.…”

Section: Introductionmentioning

confidence: 99%

Further insight for the synthesis of 6‐amino‐6‐deoxy amylose

Zhang

Shan

Liu

et al. 2020

J of Applied Polymer Sci

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Because 6-amino-6-deoxy amylose is an important intermediate for many materials, developing a practical pathway to easily prepare it in bulk is of significance. The synthesis of 6-amino-6-deoxy amylose included three reactions. In the chlorination reaction, the crude product could be fully de-esterified under the improved conditions, and the reaction mechanism was re-understood. The azido group was reduced with sodium borohydride in which the reaction conditions were studied in detail. With the improved synthetic pathway, 6-amino-6-deoxy amylose could be easily prepared. The 6-amino-6-deoxy amylose was characterized via its benzamide derivative with 1 H NMR, 1 H-13 C HSQC NMR, and 1 H-1 H COSY NMR, confirming the hydroxyl group at C 6 of amylose was converted to amino group with a degree of substitution of 98.2%. Additionally, the 6-amino-6-deoxy amylose was analyzed toward iodine reagent and X-ray diffraction, and the corresponding results showed that it was different from amylose in suprastructure and crystal structure.

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“…Despite its high transfection efficiency, viral vectors arise immunogenicity or safety concerns and are quite expensive . Hence, more and more attention has been paid to developing nonviral vectors for efficient and safe gene delivery, such as cationic liposome, polyethylenimine, poly( l ‐lysine), poly(amido amine) (PAMAM) dendrimer, etc. Among these nonviral vectors, PAMAM dendrimer, a novel class of synthetic polymer with highly branched structure, has been demonstrated to possess an immense potential in this area due to its unique intrinsic properties, such as controlled mass, surface functionality, good water solubility and biocompatibility .…”

Section: Introductionmentioning

confidence: 99%

Computer Simulation of DNA Condensation by PAMAM Dendrimer

Quan

et al. 2018

Macro Theory & Simulations

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In this study, dissipative particle dynamics is employed to investigate the complexation of poly(amido amine) dendrimer and single‐stranded DNA (ssDNA). A coarse‐grained model for ssDNA is constructed, which reproduces correctly the conformational behavior of the ssDNA molecules. The effects of pH, dendrimer generation, ionic strength, and dendrimer/ssDNA charge ratio on DNA–dendrimer complexes are explored. Simulation results show that ssDNA molecules can be significantly condensed by dendrimers and stable complexes are obtained by regulating the pH value. The ssDNA chain penetration would complicate its release from dendrimer, while this can be tuned by different generations of dendrimer. Salt concentration affects the size and stability of the complexes through ion screening effect. Dendrimer/ssDNA charge ratio can be used to control the size and morphology of the complex. This work can help design dendrimer‐based gene vectors.

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Click modification of helical amylose by poly( l -lysine) dendrons for non-viral gene delivery

Cited by 15 publications

References 23 publications

"Click" chemistry as a tool to create novel biomaterials: a short review

"Click" chemistry as a tool to create novel biomaterials: a short review

Further insight for the synthesis of 6‐amino‐6‐deoxy amylose

Computer Simulation of DNA Condensation by PAMAM Dendrimer

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